BBa_K1725100
type: ComponentDefinition
persistent identity: BBa K1725100
display id: BBa_K1725100
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressible promoter + weak RBS + PhlF repressor + terminator + PhlF repressible promoter + wea
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T04:05:17Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 27310
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1725063
component: BBa_K1725043
sequence annotation: BBa_K1725043
sequence annotation: BBa_K1725063
sequence: https://synbiohub.org/public/igem/BBa_K1725100_sequence/1

BBa_K1725063
type: Component
persistent identity: component2448693
display id: component2448693
version: 1
top level: BBa_K1725100
definition: BBa_K1725063
access: public

BBa_K1725043
type: Component
persistent identity: component2448685
display id: component2448685
version: 1
top level: BBa_K1725100
definition: BBa_K1725043
access: public

BBa_K1725043
type: SequenceAnnotation
persistent identity: annotation2448685
display id: annotation2448685
version: 1
top level: BBa_K1725100
location: https://synbiohub.org/public/igem/BBa_K1725100/annotation2448685/range2448685/1
component: BBa_K1725043

https://synbiohub.org/public/igem/BBa_K1725100/annotation2448685/range2448685/1
type: Range
persistent identity: range2448685
display id: range2448685
version: 1
top level: BBa_K1725100
direction: direction/reverse
start: 1
end: 806
orientation: inline

BBa_K1725063
type: SequenceAnnotation
persistent identity: annotation2448693
display id: annotation2448693
version: 1
top level: BBa_K1725100
location: https://synbiohub.org/public/igem/BBa_K1725100/annotation2448693/range2448693/1
component: BBa_K1725063

https://synbiohub.org/public/igem/BBa_K1725100/annotation2448693/range2448693/1
type: Range
persistent identity: range2448693
display id: range2448693
version: 1
top level: BBa_K1725100
direction: direction/reverse
start: 815
end: 1640
orientation: inline

BBa_K1725043
type: ComponentDefinition
persistent identity: BBa K1725043
display id: BBa_K1725043
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressible promoter + weak RBS + PhlF + terminator
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T03:56:02Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 27310
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1725041
component: BBa_K1725020
sequence annotation: BBa_K1725020
sequence annotation: BBa_K1725041
sequence: https://synbiohub.org/public/igem/BBa_K1725043_sequence/1

BBa_K1725041
type: Component
persistent identity: component2448631
display id: component2448631
version: 1
top level: BBa_K1725043
definition: BBa_K1725041
access: public

BBa_K1725020
type: Component
persistent identity: component2448625
display id: component2448625
version: 1
top level: BBa_K1725043
definition: P-srpR
access: public

BBa_K1725020
type: SequenceAnnotation
persistent identity: annotation2448625
display id: annotation2448625
version: 1
top level: BBa_K1725043
location: https://synbiohub.org/public/igem/BBa_K1725043/annotation2448625/range2448625/1
component: BBa_K1725020

https://synbiohub.org/public/igem/BBa_K1725043/annotation2448625/range2448625/1
type: Range
persistent identity: range2448625
display id: range2448625
version: 1
top level: BBa_K1725043
direction: direction/reverse
start: 1
end: 67
orientation: inline

BBa_K1725041
type: SequenceAnnotation
persistent identity: annotation2448631
display id: annotation2448631
version: 1
top level: BBa_K1725043
location: https://synbiohub.org/public/igem/BBa_K1725043/annotation2448631/range2448631/1
component: BBa_K1725041

https://synbiohub.org/public/igem/BBa_K1725043/annotation2448631/range2448631/1
type: Range
persistent identity: range2448631
display id: range2448631
version: 1
top level: BBa_K1725043
direction: direction/reverse
start: 76
end: 806
orientation: inline

BBa_K1725063
type: ComponentDefinition
persistent identity: BBa K1725063
display id: BBa_K1725063
version: 1
was generated by: iGEM to SBOL conversion
description: PhlF repressible promoter + weak RBS + SrpR + terminator
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T03:54:38Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 27310
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1725061
component: BBa_K1725000
sequence annotation: BBa_K1725000
sequence annotation: BBa_K1725061
sequence: https://synbiohub.org/public/igem/BBa_K1725063_sequence/1

BBa_K1725061
type: Component
persistent identity: component2448656
display id: component2448656
version: 1
top level: BBa_K1725063
definition: BBa_K1725061
access: public

BBa_K1725000
type: Component
persistent identity: component2448650
display id: component2448650
version: 1
top level: BBa_K1725063
definition: P-phlF
access: public

BBa_K1725000
type: SequenceAnnotation
persistent identity: annotation2448650
display id: annotation2448650
version: 1
top level: BBa_K1725063
location: https://synbiohub.org/public/igem/BBa_K1725063/annotation2448650/range2448650/1
component: BBa_K1725000

https://synbiohub.org/public/igem/BBa_K1725063/annotation2448650/range2448650/1
type: Range
persistent identity: range2448650
display id: range2448650
version: 1
top level: BBa_K1725063
direction: direction/reverse
start: 1
end: 48
orientation: inline

BBa_K1725061
type: SequenceAnnotation
persistent identity: annotation2448656
display id: annotation2448656
version: 1
top level: BBa_K1725063
location: https://synbiohub.org/public/igem/BBa_K1725063/annotation2448656/range2448656/1
component: BBa_K1725061

https://synbiohub.org/public/igem/BBa_K1725063/annotation2448656/range2448656/1
type: Range
persistent identity: range2448656
display id: range2448656
version: 1
top level: BBa_K1725063
direction: direction/reverse
start: 57
end: 826
orientation: inline

P-phlF
type: ComponentDefinition
persistent identity: BBa K1725000
display id: BBa_K1725000
version: 1
was generated by: iGEM to SBOL conversion
description: PhlF repressible promoter
created: 2015-08-05T11:00:00Z
modified: 2016-07-23T11:16:25Z
mutable provenance: From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2146_
m user id: 27285
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the PhlF repressor over the -10 site.
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: K1725040 operator sequence
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_K1725000_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation2476166
display id: annotation2476166
version: 1
top level: P-phlF
location: https://synbiohub.org/public/igem/BBa_K1725000/annotation2476166/range2476166/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1725000/annotation2476166/range2476166/1
type: Range
persistent identity: range2476166
display id: range2476166
version: 1
top level: P-phlF
direction: direction/forward
start: 14
end: 19
orientation: inline

K1725040 operator sequence
type: SequenceAnnotation
persistent identity: annotation2461855
display id: annotation2461855
version: 1
top level: P-phlF
location: https://synbiohub.org/public/igem/BBa_K1725000/annotation2461855/range2461855/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1725000/annotation2461855/range2461855/1
type: Range
persistent identity: range2461855
display id: range2461855
version: 1
top level: P-phlF
direction: direction/forward
start: 19
end: 48
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation2476167
display id: annotation2476167
version: 1
top level: P-phlF
location: https://synbiohub.org/public/igem/BBa_K1725000/annotation2476167/range2476167/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1725000/annotation2476167/range2476167/1
type: Range
persistent identity: range2476167
display id: range2476167
version: 1
top level: P-phlF
direction: direction/forward
start: 37
end: 42
orientation: inline

P-srpR
type: ComponentDefinition
persistent identity: BBa K1725020
display id: BBa_K1725020
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressible promoter
created: 2015-08-06T11:00:00Z
modified: 2015-09-18T03:58:50Z
mutable provenance: From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 20827
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the SrpR repressor over the -10 site.
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: K1725060 operator sequence
sequence: https://synbiohub.org/public/igem/BBa_K1725020_sequence/1

K1725060 operator sequence
type: SequenceAnnotation
persistent identity: annotation2461859
display id: annotation2461859
version: 1
top level: P-srpR
location: https://synbiohub.org/public/igem/BBa_K1725020/annotation2461859/range2461859/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1725020/annotation2461859/range2461859/1
type: Range
persistent identity: range2461859
display id: range2461859
version: 1
top level: P-srpR
direction: direction/forward
start: 38
end: 67
orientation: inline

BBa_K1725061
type: ComponentDefinition
persistent identity: BBa K1725061
display id: BBa_K1725061
version: 1
was generated by: iGEM to SBOL conversion
description: srpR with RBS and terminator
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T02:59:07Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: srpR repressor (K1725060) with ribosome binding site B0032 (0.3 of B0034) and terminator K1725081
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 27284
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1725081
component: BBa_K1725060
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K1725060
sequence annotation: BBa_K1725081
sequence: https://synbiohub.org/public/igem/BBa_K1725061_sequence/1

BBa_K1725081
type: Component
persistent identity: component2448642
display id: component2448642
version: 1
top level: BBa_K1725061
definition: BBa_K1725081
access: public

BBa_K1725060
type: Component
persistent identity: component2448641
display id: component2448641
version: 1
top level: BBa_K1725061
definition: srpR
access: public

BBa_B0032
type: Component
persistent identity: component2448639
display id: component2448639
version: 1
top level: BBa_K1725061
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2448639
display id: annotation2448639
version: 1
top level: BBa_K1725061
location: https://synbiohub.org/public/igem/BBa_K1725061/annotation2448639/range2448639/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1725061/annotation2448639/range2448639/1
type: Range
persistent identity: range2448639
display id: range2448639
version: 1
top level: BBa_K1725061
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1725060
type: SequenceAnnotation
persistent identity: annotation2448641
display id: annotation2448641
version: 1
top level: BBa_K1725061
location: https://synbiohub.org/public/igem/BBa_K1725061/annotation2448641/range2448641/1
component: BBa_K1725060

https://synbiohub.org/public/igem/BBa_K1725061/annotation2448641/range2448641/1
type: Range
persistent identity: range2448641
display id: range2448641
version: 1
top level: BBa_K1725061
direction: direction/reverse
start: 20
end: 661
orientation: inline

BBa_K1725081
type: SequenceAnnotation
persistent identity: annotation2448642
display id: annotation2448642
version: 1
top level: BBa_K1725061
location: https://synbiohub.org/public/igem/BBa_K1725061/annotation2448642/range2448642/1
component: BBa_K1725081

https://synbiohub.org/public/igem/BBa_K1725061/annotation2448642/range2448642/1
type: Range
persistent identity: range2448642
display id: range2448642
version: 1
top level: BBa_K1725061
direction: direction/reverse
start: 670
end: 770
orientation: inline

BBa_K1725041
type: ComponentDefinition
persistent identity: BBa K1725041
display id: BBa_K1725041
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + PhlF repressor + terminator
created: 2015-08-10T11:00:00Z
modified: 2015-08-11T10:49:59Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: PhlF repressor from Pseudomonas protegens Pf-5, with RBS B0032, and terminator K1725081
discontinued: false
dominant: false
group u list: _2146_
m user id: 27284
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_K1725081
component: BBa_K1725040
sequence annotation: BBa_B0032
sequence annotation: BBa_K1725040
sequence annotation: BBa_K1725081
sequence: https://synbiohub.org/public/igem/BBa_K1725041_sequence/1

BBa_B0032
type: Component
persistent identity: component2435063
display id: component2435063
version: 1
top level: BBa_K1725041
definition: BBa_B0032
access: public

BBa_K1725081
type: Component
persistent identity: component2435066
display id: component2435066
version: 1
top level: BBa_K1725041
definition: BBa_K1725081
access: public

BBa_K1725040
type: Component
persistent identity: component2435065
display id: component2435065
version: 1
top level: BBa_K1725041
definition: phlF
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2435063
display id: annotation2435063
version: 1
top level: BBa_K1725041
location: https://synbiohub.org/public/igem/BBa_K1725041/annotation2435063/range2435063/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1725041/annotation2435063/range2435063/1
type: Range
persistent identity: range2435063
display id: range2435063
version: 1
top level: BBa_K1725041
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1725040
type: SequenceAnnotation
persistent identity: annotation2435065
display id: annotation2435065
version: 1
top level: BBa_K1725041
location: https://synbiohub.org/public/igem/BBa_K1725041/annotation2435065/range2435065/1
component: BBa_K1725040

https://synbiohub.org/public/igem/BBa_K1725041/annotation2435065/range2435065/1
type: Range
persistent identity: range2435065
display id: range2435065
version: 1
top level: BBa_K1725041
direction: direction/reverse
start: 20
end: 622
orientation: inline

BBa_K1725081
type: SequenceAnnotation
persistent identity: annotation2435066
display id: annotation2435066
version: 1
top level: BBa_K1725041
location: https://synbiohub.org/public/igem/BBa_K1725041/annotation2435066/range2435066/1
component: BBa_K1725081

https://synbiohub.org/public/igem/BBa_K1725041/annotation2435066/range2435066/1
type: Range
persistent identity: range2435066
display id: range2435066
version: 1
top level: BBa_K1725041
direction: direction/reverse
start: 631
end: 731
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

srpR
type: ComponentDefinition
persistent identity: BBa K1725060
display id: BBa_K1725060
version: 1
was generated by: iGEM to SBOL conversion
description: SrpR repressor from Pseudomonas putida
created: 2015-08-11T11:00:00Z
modified: 2015-09-18T02:13:41Z
mutable provenance: Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: SrpR repressor from Pseudomonas putida. Represses promoter K1725020.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 20827
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Removed 3 Spe1 restriction sites to make it BioBrick compatible.
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: srpR
sequence: https://synbiohub.org/public/igem/BBa_K1725060_sequence/1

srpR
type: SequenceAnnotation
persistent identity: annotation2473681
display id: annotation2473681
version: 1
top level: srpR
location: https://synbiohub.org/public/igem/BBa_K1725060/annotation2473681/range2473681/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1725060/annotation2473681/range2473681/1
type: Range
persistent identity: range2473681
display id: range2473681
version: 1
top level: srpR
direction: direction/forward
start: 1
end: 639
orientation: inline

BBa_K1725081
type: ComponentDefinition
persistent identity: BBa K1725081
display id: BBa_K1725081
version: 1
was generated by: iGEM to SBOL conversion
description: T1 terminator from E. coli rrnB
created: 2015-08-10T11:00:00Z
modified: 2016-07-23T11:20:14Z
mutable provenance: T1 terminator from E. coli rrnB
owned by: james
owned by: myers
mutable description: T1 terminator from E. coli rrnB
discontinued: false
dominant: false
experience: experience/Works
group u list: _2146_
m user id: 27285
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B0010
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1725081_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2476137
display id: annotation2476137
version: 1
top level: BBa_K1725081
location: https://synbiohub.org/public/igem/BBa_K1725081/annotation2476137/range2476137/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1725081/annotation2476137/range2476137/1
type: Range
persistent identity: range2476137
display id: range2476137
version: 1
top level: BBa_K1725081
direction: direction/forward
start: 1
end: 80
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2476138
display id: annotation2476138
version: 1
top level: BBa_K1725081
location: https://synbiohub.org/public/igem/BBa_K1725081/annotation2476138/range2476138/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1725081/annotation2476138/range2476138/1
type: Range
persistent identity: range2476138
display id: range2476138
version: 1
top level: BBa_K1725081
direction: direction/forward
start: 12
end: 56
orientation: inline

phlF
type: ComponentDefinition
persistent identity: BBa K1725040
display id: BBa_K1725040
version: 1
was generated by: iGEM to SBOL conversion
description: phlF encoding PhlF repressor from Pseudomonas protegens Pf-5
created: 2015-08-10T11:00:00Z
modified: 2016-07-23T11:16:49Z
mutable provenance: Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: PhlF repressor from Pseudomonas protegens Pf-5. Represses promoter K1725000.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2146_
m user id: 27285
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: phlF
sequence: https://synbiohub.org/public/igem/BBa_K1725040_sequence/1

phlF
type: SequenceAnnotation
persistent identity: annotation2473659
display id: annotation2473659
version: 1
top level: phlF
location: https://synbiohub.org/public/igem/BBa_K1725040/annotation2473659/range2473659/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1725040/annotation2473659/range2473659/1
type: Range
persistent identity: range2473659
display id: range2473659
version: 1
top level: phlF
direction: direction/forward
start: 1
end: 600
orientation: inline

https://synbiohub.org/public/igem/BBa_K1725100_sequence/1
type: Sequence
persistent identity: BBa K1725100 sequence
display id: BBa_K1725100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagtcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgtactagaggattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725043_sequence/1
type: Sequence
persistent identity: BBa K1725043 sequence
display id: BBa_K1725043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagtcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725063_sequence/1
type: Sequence
persistent identity: BBa K1725063 sequence
display id: BBa_K1725063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725000_sequence/1
type: Sequence
persistent identity: BBa K1725000 sequence
display id: BBa_K1725000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725020_sequence/1
type: Sequence
persistent identity: BBa K1725020 sequence
display id: BBa_K1725020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725061_sequence/1
type: Sequence
persistent identity: BBa K1725061 sequence
display id: BBa_K1725061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725041_sequence/1
type: Sequence
persistent identity: BBa K1725041 sequence
display id: BBa_K1725041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725060_sequence/1
type: Sequence
persistent identity: BBa K1725060 sequence
display id: BBa_K1725060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725081_sequence/1
type: Sequence
persistent identity: BBa K1725081 sequence
display id: BBa_K1725081_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725040_sequence/1
type: Sequence
persistent identity: BBa K1725040 sequence
display id: BBa_K1725040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z