LOCUS       BBa_K1725101            1640 bp    DNA     linear       06-Dec-2025
DEFINITION  PhlF repressible promoter + weak RBS + SrpR repressor + terminator 
            + SrpR repressible promoter + wea
ACCESSION   BBa_K1725101
VERSION     BBa_K1725101.1  
FEATURES             Location/Qualifiers
     misc_feature    19..48
                     /label=K1725040 operator sequence
     misc_feature    14..19
                     /label=-35 site
     misc_feature    37..42
                     /label=-10 site
     promoter        1..48
                     /label=BBa_K1725000
     RBS             57..69
                     /label=RBS-3\Weak
     RBS             63..66
                     /label=RBS
     misc_feature    57..69
                     /label=BBa_B0032
     RBS             57..69
                     /label=BBa_B0032
     CDS             76..714
                     /label=srpR
     CDS             76..717
                     /label=BBa_K1725060
     misc_feature    726..805
                     /label=B0010
     misc_feature    737..781
                     /label=T1
     terminator      726..826
                     /label=BBa_K1725081
     misc_feature    57..826
                     /label=BBa_K1725061
     misc_feature    1..826
                     /label=BBa_K1725063
     misc_feature    872..901
                     /label=K1725060 operator sequence
     promoter        835..901
                     /label=BBa_K1725020
     RBS             910..922
                     /label=RBS-3\Weak
     RBS             916..919
                     /label=RBS
     misc_feature    910..922
                     /label=BBa_B0032
     RBS             910..922
                     /label=BBa_B0032
     CDS             929..1528
                     /label=phlF
     CDS             929..1531
                     /label=BBa_K1725040
     misc_feature    1540..1619
                     /label=B0010
     misc_feature    1551..1595
                     /label=T1
     terminator      1540..1640
                     /label=BBa_K1725081
     misc_feature    910..1640
                     /label=BBa_K1725041
     misc_feature    835..1640
                     /label=BBa_K1725043
ORIGIN
        1 gattcgttac caattgacat gatacgaaac gtaccgtatc gttaaggtta ctagagtcac
       61 acaggaaagt actagatggc acgtaaaacc gcagcagaag cagaagaaac ccgtcagcgt
      121 attattgatg cagcactgga agtttttgtt gcacagggtg ttagtgatgc aaccctggat
      181 cagattgcac gtaaagccgg tgttacccgt ggtgcagttt attggcattt taatggtaaa
      241 ctggaagttc tgcaagcagt tctggcaagc cgtcagcatc cgctggaact ggattttaca
      301 ccggatctgg gtattgaacg tagctgggaa gcagttgttg ttgcaatgct ggatgcagtt
      361 catagtccgc agagcaaaca gtttagcgaa attctgattt atcagggtct ggatgaaagc
      421 ggtctgattc ataatcgtat ggttcaggca agcgatcgtt ttctgcaata tattcatcag
      481 gttctgcgtc atgcagttac ccagggtgaa ctgccgatta atctggatct gcaaaccagc
      541 attggtgttt ttaaaggtct gattaccggt ctgctgtatg aaggtctgcg tagcaaagat
      601 cagcaggcac agattatcaa agttgcactg ggtagctttt gggcactgct gcgtgaaccg
      661 cctcgttttc tgctgtgtga agaagcacag attaaacagg tgaaatcctt cgaataatac
      721 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      781 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgtact agaggattcg
      841 ttaccaattg acagctagct cagtcctagg tatatacata catgcttgtt tgtttgtaaa
      901 ctactagagt cacacaggaa agtactagat ggcacgtacc ccgagccgta gcagcattgg
      961 tagcctgcgt agtccgcata cccataaagc aattctgacc agcaccattg aaatcctgaa
     1021 agaatgtggt tatagcggtc tgagcattga aagcgttgca cgtcgtgccg gtgcaagcaa
     1081 accgaccatt tatcgttggt ggaccaataa agcagcactg attgccgaag tgtatgaaaa
     1141 tgaaagcgaa caggtgcgta aatttccgga tctgggtagc tttaaagccg atctggattt
     1201 tctgctgcgt aatctgtgga aagtttggcg tgaaaccatt tgtggtgaag catttcgttg
     1261 tgttattgca gaagcacagc tggaccctgc aaccctgacc cagctgaaag atcagtttat
     1321 ggaacgtcgt cgtgagatgc cgaaaaaact ggttgaaaat gccattagca atggtgaact
     1381 gccgaaagat accaatcgtg aactgctgct ggatatgatt tttggttttt gttggtatcg
     1441 cctgctgacc gaacagctga ccgttgaaca ggatattgaa gaatttacct tcctgctgat
     1501 taatggtgtt tgtccgggta cacagcgtta atactagagc caggcatcaa ataaaacgaa
     1561 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     1621 tgagtaggac aaatccgccg
//