LOCUS       BBa_K1725103            1742 bp    DNA     linear       17-Jan-2026
DEFINITION  SrpR repressible promoter + strong RBS + RFP + terminator + PhlF 
            repressible promoter + weak RBS + G
ACCESSION   BBa_K1725103
VERSION     BBa_K1725103.1  
FEATURES             Location/Qualifiers
     misc_feature    38..67
                     /label=K1725060 operator sequence
     promoter        1..67
                     /label=BBa_K1725020
     RBS             76..90
                     /label=RBS-1\Strong
     RBS             83..87
                     /label=RBS
     misc_feature    76..90
                     /label=BBa_B0030
     RBS             76..90
                     /label=BBa_B0030
     CDS             97..771
                     /label=mrfp1
     misc_feature    778..802
                     /label=Help:Barcodes
     CDS             97..802
                     /label=BBa_E1010
     stem_loop       822..865
                     /label=stem_loop
     misc_feature    811..890
                     /label=BBa_B0010
     terminator      811..890
                     /label=BBa_B0010
     stem_loop       906..925
                     /label=T7 TE
     misc_feature    932..932
                     /label=stop
     polyA_site      926..939
                     /label=polya
     misc_feature    899..939
                     /label=BBa_B0012
     terminator      899..939
                     /label=BBa_B0012
     terminator      811..939
                     /label=BBa_B0015
     misc_feature    76..939
                     /label=BBa_K516030
     misc_feature    1..939
                     /label=BBa_K1725102
     misc_feature    966..995
                     /label=K1725040 operator sequence
     misc_feature    961..966
                     /label=-35 site
     misc_feature    984..989
                     /label=-10 site
     promoter        948..995
                     /label=BBa_K1725000
     RBS             1004..1016
                     /label=RBS-3\Weak
     RBS             1010..1013
                     /label=RBS
     misc_feature    1004..1016
                     /label=BBa_B0032
     RBS             1004..1016
                     /label=BBa_B0032
     CDS             1023..1742
                     /label=GFP protein
     CDS             1023..1742
                     /label=BBa_E0040
     misc_feature    1004..1742
                     /label=BBa_E5501
     misc_feature    948..1742
                     /label=BBa_K1725002
ORIGIN
        1 gattcgttac caattgacag ctagctcagt cctaggtata tacatacatg cttgtttgtt
       61 tgtaaactac tagagattaa agaggagaaa tactagatgg cttcctccga agacgttatc
      121 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
      181 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
      241 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
      301 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
      361 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
      421 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
      481 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
      541 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
      601 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
      661 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
      721 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
      781 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      901 acactggctc accttcgggt gggcctttct gcgtttatat actagaggat tcgttaccaa
      961 ttgacatgat acgaaacgta ccgtatcgtt aaggttacta gagtcacaca ggaaagtact
     1021 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1081 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1141 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1201 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1261 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1321 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1381 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1441 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1501 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1561 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1621 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1681 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1741 aa
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