##gff-version 3
##sequence-region BBa_K1725103 1 1742
BBa_K1725103	.	engineered_region	1	939	.	+	0	ID=BBa_K1725102;Name=BBa_K1725102
BBa_K1725103	.	promoter	1	67	.	+	0	ID=BBa_K1725020;Name=BBa_K1725020;Parent=BBa_K1725102
BBa_K1725103	.	operator	38	67	.	+	0	ID=annotation2461859;Name=K1725060 operator sequence;Parent=BBa_K1725020
BBa_K1725103	.	engineered_region	76	939	.	+	0	ID=BBa_K516030;Name=BBa_K516030;Parent=BBa_K1725102
BBa_K1725103	.	terminator	811	939	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516030
BBa_K1725103	.	terminator	811	890	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1725103	.	stem_loop	822	865	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1725103	.	engineered_region	811	890	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1725103	.	terminator	899	939	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1725103	.	engineered_region	899	939	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1725103	.	stop_codon	932	932	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1725103	.	polyA_site	926	939	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1725103	.	stem_loop	906	925	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1725103	.	CDS	97	802	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K516030
BBa_K1725103	.	CDS	97	771	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1725103	.	engineered_tag	778	802	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1725103	.	ribosome_entry_site	76	90	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K516030
BBa_K1725103	.	engineered_region	76	90	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1725103	.	ribosome_entry_site	76	90	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1725103	.	ribosome_entry_site	83	87	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1725103	.	engineered_region	948	1742	.	+	0	ID=BBa_K1725002;Name=BBa_K1725002
BBa_K1725103	.	engineered_region	1004	1742	.	+	0	ID=BBa_E5501;Name=BBa_E5501;Parent=BBa_K1725002
BBa_K1725103	.	ribosome_entry_site	1004	1016	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E5501
BBa_K1725103	.	ribosome_entry_site	1010	1013	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725103	.	ribosome_entry_site	1004	1016	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725103	.	engineered_region	1004	1016	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725103	.	CDS	1023	1742	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5501
BBa_K1725103	.	CDS	1023	1742	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1725103	.	promoter	948	995	.	+	0	ID=BBa_K1725000;Name=BBa_K1725000;Parent=BBa_K1725002
BBa_K1725103	.	non_covalent_binding_site	984	989	.	+	0	ID=annotation2476167;Name=-10 site;Parent=BBa_K1725000
BBa_K1725103	.	non_covalent_binding_site	961	966	.	+	0	ID=annotation2476166;Name=-35 site;Parent=BBa_K1725000
BBa_K1725103	.	operator	966	995	.	+	0	ID=annotation2461855;Name=K1725040 operator sequence;Parent=BBa_K1725000
>BBa_K1725103
gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagatta
aagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttc
cgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaa
gttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtta
aacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcga
agacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggt
accaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccgg
aagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaac
cacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaac
gaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgct
agtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagg
attcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaata
ataa