LOCUS       BBa_K1725214            3310 bp    DNA     linear       25-Nov-2024
DEFINITION  LuxA + LuxB with pBAD
ACCESSION   BBa_K1725214
VERSION     BBa_K1725214.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1231
                     /label=RBS-3\Weak
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1231
                     /label=BBa_B0032
     RBS             1219..1231
                     /label=BBa_B0032
     CDS             1238..2302
                     /label=LuxA
     CDS             1238..2302
                     /label=BBa_K1725200
     RBS             2311..2323
                     /label=RBS-3\Weak
     RBS             2317..2320
                     /label=RBS
     misc_feature    2311..2323
                     /label=BBa_B0032
     RBS             2311..2323
                     /label=BBa_B0032
     CDS             2330..3310
                     /label=LuxB
     CDS             2330..3310
                     /label=BBa_K1725201
     misc_feature    1219..3310
                     /label=BBa_K1725212
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa gtactagatg aagtttggaa atatttgttt
     1261 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg
     1321 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga
     1381 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac
     1441 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga
     1501 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg
     1561 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac
     1621 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag
     1681 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc
     1741 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc
     1801 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa
     1861 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat
     1921 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa
     1981 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg
     2041 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa
     2101 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga
     2161 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc
     2221 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc
     2281 tttcttaaaa gaacctaaat aatactagag tcacacagga aagtactaga tgaaatttgg
     2341 attatttttt ctaaactttc agaaagatgg aataacatct gaagaaacgt tggataatat
     2401 ggtaaagact gtcacgttaa ttgattcaac taaatatcat tttaatactg cctttgttaa
     2461 tgaacatcac ttttcaaaaa atggtattgt tggagcacct attaccgcag ctggtttttt
     2521 attagggtta acaaataaat tacatattgg ttcattaaat caagtaatta ccacccatca
     2581 ccctgtacgt gtagcagaag aagccagttt attagatcaa atgtcagagg gacgcttcat
     2641 tcttggtttt agtgactgcg aaagtgattt cgaaatggaa ttttttagac gtcatatctc
     2701 atcaaggcaa caacaatttg aagcatgcta tgaaataatt aatgacgcat taactacagg
     2761 ttattgtcat ccccaaaacg acttttatga ttttccaaag gtttcaatta atccacactg
     2821 ttacagtgag aatggaccta agcaatatgt atccgctaca tcaaaagaag tcgtcatgtg
     2881 ggcagcgaaa aaggcactgc ctttaacatt taagtgggag gataatttag aaaccaaaga
     2941 acgctatgca attctatata ataaaacagc acaacaatat ggtattgata tttcggatgt
     3001 tgatcatcaa ttaactgtaa ttgcgaactt aaatgctgat agaagtacgg ctcaagaaga
     3061 agtgagagaa tacttaaaag actatatcac tgaaacttac cctcaaatgg acagagatga
     3121 aaaaattaac tgcattattg aagagaatgc agttgggtct catgatgact attatgaatc
     3181 gacaaaatta gcagtggaaa aaacagggtc taaaaatatt ttattatcct ttgaatcaat
     3241 gtccgatatt aaagatgtaa aagatattat tgatatgttg aaccaaaaaa tcgaaatgaa
     3301 tttaccataa
//