LOCUS       BBa_K1725215            2479 bp    DNA     linear       03-May-2024
DEFINITION  LuxC + LuxD with BBa_K1725080 lac regulated promoter
ACCESSION   BBa_K1725215
VERSION     BBa_K1725215.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=NheI site
     promoter        7..61
                     /label=PLlac 0-1 promoter
     promoter        1..61
                     /label=BBa_K1725080
     RBS             70..82
                     /label=RBS-3\Weak
     RBS             76..79
                     /label=RBS
     misc_feature    70..82
                     /label=BBa_B0032
     RBS             70..82
                     /label=BBa_B0032
     CDS             89..1528
                     /label=LuxC
     CDS             89..1528
                     /label=BBa_K1725202
     RBS             1537..1549
                     /label=RBS-3\Weak
     RBS             1543..1546
                     /label=RBS
     misc_feature    1537..1549
                     /label=BBa_B0032
     RBS             1537..1549
                     /label=BBa_B0032
     CDS             1556..2479
                     /label=LuxD
     CDS             1556..2479
                     /label=BBa_K1725203
     misc_feature    70..2479
                     /label=BBa_K1725213
ORIGIN
        1 gctagcaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atactagagt cacacaggaa agtactagat gaataaatgt attccaatga taattaatgg
      121 aatgattcaa gattttgata attatgcata taaagaagtt aaactaaata atgataatag
      181 agtaaaatta tctgtcatta ctgaaagttc agtttcaaaa acattaaata tcaaagatag
      241 aattaatcta aatttaaatc agattgtgaa ttttttatat accgttggtc aacgatggaa
      301 aagtgaagaa tataatcggc gacgaaccta tatccgtgag ttaaaaacat atcttggtta
      361 ttctgatgaa atggcaagat tagaagcgaa ttggattgca atgttattgt gctctaaaag
      421 tgctttgtat gacattgtta attatgattt gggctctata cacgtattag atgaatggct
      481 tccacgtggt gattgctatg ttaaagcaca accgaaaggt gtttctgttc acttgttagc
      541 tggtaatgtt ccattatcag gagtgacatc tattttgcgt gctattttaa caaaaaatga
      601 gtgcattatt aaaacttcgt cttcagatcc ttttactgca aacgctttag tttccagttt
      661 tattgatgtt aatgcagacc atccaataac caaatcaatg tctgttatgt attggccgca
      721 tgatgaagat atgactctat ctcaaagaat aatgaatcat gccgacgtgg ttattgcttg
      781 gggtggagac gaggcgatta aatgggcggt aaaatattca ccaccgcatg tcgatattct
      841 gaaatttgga ccaaagaaaa gcttaagtat tattgaagct cctaaagata tagaagccgc
      901 agcaatgggg gttgctcatg atatttgttt ctatgaccag caagcctgct tctctactca
      961 agacgtttat tatataggag ataatttacc tttattttta aatgaacttg aaaaacagct
     1021 agatcgatac gcgaaaattt taccaaaagg ttcaaatagt tttgatgaaa aagcggcgtt
     1081 tactcttact gaaaaagaaa gtctatttgc tggatatgaa gtgagaaagg gagataagca
     1141 agcttggtta atagtcgtat cacctacaaa tagctttgga aatcaaccgc tatcacgaag
     1201 tgtgtatgtt catcaagtat ctgatattaa agagataatt ccttttgtta ataaaaatag
     1261 aacacaaact gtttctattt atccttggga agcgtcatta aaatatcgag ataaattagc
     1321 aagaagtgga gttgaaagaa ttgttgaatc aggcatgaat aatattttca gagttggagg
     1381 ggctcatgat tcattatctc ctctccagta cctagttagg tttgtatcgc atgagagacc
     1441 atttaattat acgacaaaag atgttgcggt tgaaatcgaa caaacacgtt acttagagga
     1501 agataagttt ttagtttttg tcccataata ctagagtcac acaggaaagt actagatgaa
     1561 agatgaaagt gcttttttta cgattgatca catcatcaag cttgataatg gtcagtctat
     1621 ccgagtttgg gaaacactcc ctaaaaagaa cgtaccagag aaaaaacata caatacttat
     1681 tgcttcgggt tttgctagaa gaatggatca ttttgcaggt cttgctgagt atttatctac
     1741 taacggtttt catgtcattc gctacgattc tttgcatcat gttggattaa gcagtggatg
     1801 tataaatgaa tttacgatgt cgattggaaa aaatagcctg cttacagtcg tagattggct
     1861 tacagatcat ggtgtcgaac gaatagggct gattgctgct agtttgtcag cgagaatcgc
     1921 ctatgaggta gtaaataaaa ttaaattatc atttttaatt acggccgtag gtgtcgttaa
     1981 tcttagagat acattagaaa aagcattgga gtatgactat ttgcaattac ctatttcaga
     2041 gttaccagaa gatcttgact ttgaaggtca taatttagga tcggaggtct ttgttacaga
     2101 ttgctttaaa cataattggg acacgttaga ctcgacactt aatagtgtta aaggattagc
     2161 gattccattt attgctttta ctgcaaacga tgatagttgg gtaaagcaaa gtgaagttat
     2221 agagctcatt gatagcattg aatctagtaa ttgtaagctc tattcgctaa ttggaagttc
     2281 acatgatctt ggggaaaatt tggttgtatt aagaaatttt tatcaatcag tgacgaaggc
     2341 agccttagca ttagatgatg gtttattgga tttagagata gacattattg aacctcgatt
     2401 tgaggacgtt acaagtatta ctgttaagga gcgtagatta aaaaatgaaa ttgaaaatga
     2461 attattagaa ttggcttaa
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