LOCUS BBa_K1725219 3356 bp DNA linear 23-Dec-2024 DEFINITION LuxCDE with R0010 ACCESSION BBa_K1725219 VERSION BBa_K1725219.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1667 /label=LuxC CDS 228..1667 /label=BBa_K1725202 RBS 1676..1688 /label=RBS-3\Weak RBS 1682..1685 /label=RBS misc_feature 1676..1688 /label=BBa_B0032 RBS 1676..1688 /label=BBa_B0032 CDS 1695..2618 /label=LuxD CDS 1695..2618 /label=BBa_K1725203 misc_feature 209..2618 /label=BBa_K1725213 RBS 2627..2639 /label=RBS-3\Weak RBS 2633..2636 /label=RBS misc_feature 2627..2639 /label=BBa_B0032 RBS 2627..2639 /label=BBa_B0032 CDS 2646..3356 /label=LuxG CDS 2646..3356 /label=BBa_K1725205 misc_feature 2627..3356 /label=BBa_K1725211 misc_feature 209..3356 /label=BBa_K1725217 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg aataaatgta 241 ttccaatgat aattaatgga atgattcaag attttgataa ttatgcatat aaagaagtta 301 aactaaataa tgataataga gtaaaattat ctgtcattac tgaaagttca gtttcaaaaa 361 cattaaatat caaagataga attaatctaa atttaaatca gattgtgaat tttttatata 421 ccgttggtca acgatggaaa agtgaagaat ataatcggcg acgaacctat atccgtgagt 481 taaaaacata tcttggttat tctgatgaaa tggcaagatt agaagcgaat tggattgcaa 541 tgttattgtg ctctaaaagt gctttgtatg acattgttaa ttatgatttg ggctctatac 601 acgtattaga tgaatggctt ccacgtggtg attgctatgt taaagcacaa ccgaaaggtg 661 tttctgttca cttgttagct ggtaatgttc cattatcagg agtgacatct attttgcgtg 721 ctattttaac aaaaaatgag tgcattatta aaacttcgtc ttcagatcct tttactgcaa 781 acgctttagt ttccagtttt attgatgtta atgcagacca tccaataacc aaatcaatgt 841 ctgttatgta ttggccgcat gatgaagata tgactctatc tcaaagaata atgaatcatg 901 ccgacgtggt tattgcttgg ggtggagacg aggcgattaa atgggcggta aaatattcac 961 caccgcatgt cgatattctg aaatttggac caaagaaaag cttaagtatt attgaagctc 1021 ctaaagatat agaagccgca gcaatggggg ttgctcatga tatttgtttc tatgaccagc 1081 aagcctgctt ctctactcaa gacgtttatt atataggaga taatttacct ttatttttaa 1141 atgaacttga aaaacagcta gatcgatacg cgaaaatttt accaaaaggt tcaaatagtt 1201 ttgatgaaaa agcggcgttt actcttactg aaaaagaaag tctatttgct ggatatgaag 1261 tgagaaaggg agataagcaa gcttggttaa tagtcgtatc acctacaaat agctttggaa 1321 atcaaccgct atcacgaagt gtgtatgttc atcaagtatc tgatattaaa gagataattc 1381 cttttgttaa taaaaataga acacaaactg tttctattta tccttgggaa gcgtcattaa 1441 aatatcgaga taaattagca agaagtggag ttgaaagaat tgttgaatca ggcatgaata 1501 atattttcag agttggaggg gctcatgatt cattatctcc tctccagtac ctagttaggt 1561 ttgtatcgca tgagagacca tttaattata cgacaaaaga tgttgcggtt gaaatcgaac 1621 aaacacgtta cttagaggaa gataagtttt tagtttttgt cccataatac tagagtcaca 1681 caggaaagta ctagatgaaa gatgaaagtg ctttttttac gattgatcac atcatcaagc 1741 ttgataatgg tcagtctatc cgagtttggg aaacactccc taaaaagaac gtaccagaga 1801 aaaaacatac aatacttatt gcttcgggtt ttgctagaag aatggatcat tttgcaggtc 1861 ttgctgagta tttatctact aacggttttc atgtcattcg ctacgattct ttgcatcatg 1921 ttggattaag cagtggatgt ataaatgaat ttacgatgtc gattggaaaa aatagcctgc 1981 ttacagtcgt agattggctt acagatcatg gtgtcgaacg aatagggctg attgctgcta 2041 gtttgtcagc gagaatcgcc tatgaggtag taaataaaat taaattatca tttttaatta 2101 cggccgtagg tgtcgttaat cttagagata cattagaaaa agcattggag tatgactatt 2161 tgcaattacc tatttcagag ttaccagaag atcttgactt tgaaggtcat aatttaggat 2221 cggaggtctt tgttacagat tgctttaaac ataattggga cacgttagac tcgacactta 2281 atagtgttaa aggattagcg attccattta ttgcttttac tgcaaacgat gatagttggg 2341 taaagcaaag tgaagttata gagctcattg atagcattga atctagtaat tgtaagctct 2401 attcgctaat tggaagttca catgatcttg gggaaaattt ggttgtatta agaaattttt 2461 atcaatcagt gacgaaggca gccttagcat tagatgatgg tttattggat ttagagatag 2521 acattattga acctcgattt gaggacgtta caagtattac tgttaaggag cgtagattaa 2581 aaaatgaaat tgaaaatgaa ttattagaat tggcttaata ctagagtcac acaggaaagt 2641 actagatgat tgttgatggt agagtttcaa aaatagtatt agcgtcaata agaaataata 2701 tatataaggt atttattact gtaaattcac caataaagtt catcgctgga caatttgtaa 2761 tggtcacgat taatgggaaa aaatgccctt tttcaattgc gaattgcccg acaaaaaatt 2821 acgaaataga attgcatatt ggtagttcga atagagactg ctcattggat attatcgaat 2881 attttgtcga tgctcttgtt gaggaagtcg caattgagtt agatgctccc catggaaacg 2941 cttggttacg gtctgaaagt aataacccat tgctattaat tgcgggaggt acaggtttat 3001 catatataaa tagcattcta acaaattgct taaataggaa tatacctcaa gatatttatc 3061 tttactgggg agtaaaagac agttctcttt tgtatgaaga tgaagagtta ctaaacttat 3121 cactaaacaa caaaaacttt cattatattc ctgttattga agataaaagt gaagaatgga 3181 tagggagaaa aggcactgtt cttgatgctg tcatggaaga ttttactgat cttacttatt 3241 ttgatattta tgtttgtgga cccttcatga tggctaaaac agcaaaagaa aaattaattg 3301 aagagaaaaa agcaaagtca gaacagatgt ttgccgatgc ttttgcatac gtataa //