BBa_K1725211 1 BBa_K1725211 B0032 + LuxG 2015-08-09T11:00:00Z 2015-08-10T08:06:09Z Vibrio fischeri regulatory protein LuxR (luxR) gene LuxG with ribosome binding site B0032 false false _2146_ 27303 27303 9 false None false Charlotte Flynn component2434678 1 BBa_B0032 component2434681 1 BBa_K1725205 annotation2434678 1 BBa_B0032 range2434678 1 1 13 annotation2434681 1 BBa_K1725205 range2434681 1 20 730 BBa_K1725205 1 luxG LuxG 2015-08-03T11:00:00Z 2015-09-18T11:35:46Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434651 1 LuxG range2434651 1 1 711 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K1725203 1 luxD LuxD 2015-08-03T11:00:00Z 2015-09-18T11:34:37Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434649 1 LuxD range2434649 1 1 924 BBa_K1725217 1 BBa_K1725217 LuxCDE with B0032 2015-08-09T11:00:00Z 2015-08-26T05:34:42Z Vibrio fischeri regulatory protein LuxR (luxR) gene LuxC, LuxD and LuxE with the ribosome binding site B0032. true false _2146_ 27288 27303 9 false None false Charlotte Flynn component2434762 1 BBa_K1725213 component2434768 1 BBa_K1725211 annotation2434762 1 BBa_K1725213 range2434762 1 1 2410 annotation2434768 1 BBa_K1725211 range2434768 1 2419 3148 BBa_K1725213 1 BBa_K1725213 LuxC + LuxD both with B0032 2015-08-09T11:00:00Z 2015-09-18T11:15:48Z Vibrio fischeri regulatory protein LuxR (luxR) gene LuxC and LuxD both with ribosome binding site B0032. false false _2146_ 20827 27303 9 false None false Charlotte Flynn component2434696 1 BBa_K1725202 component2434698 1 BBa_B0032 component2434693 1 BBa_B0032 component2434701 1 BBa_K1725203 annotation2434693 1 BBa_B0032 range2434693 1 1 13 annotation2434696 1 BBa_K1725202 range2434696 1 20 1459 annotation2434701 1 BBa_K1725203 range2434701 1 1487 2410 annotation2434698 1 BBa_B0032 range2434698 1 1468 1480 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K1725202 1 luxC LuxC 2015-08-03T11:00:00Z 2015-09-18T11:34:09Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434648 1 LuxC range2434648 1 1 1440 BBa_K1725219 1 BBa_K1725219 LuxCDE with R0010 2015-08-09T11:00:00Z 2015-08-10T09:24:46Z Vibrio fischeri regulatory protein LuxR (luxR) gene LuxC, LuxD and LuxE with the promoter R0010 false false _2146_ 27303 27303 9 false None false Charlotte Flynn component2434815 1 BBa_K1725217 component2434791 1 BBa_R0010 annotation2434791 1 BBa_R0010 range2434791 1 1 200 annotation2434815 1 BBa_K1725217 range2434815 1 209 3356 BBa_K1725205_sequence 1 atgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa BBa_K1725202_sequence 1 atgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1725211_sequence 1 tcacacaggaaagtactagatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa BBa_K1725203_sequence 1 atgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaa BBa_K1725217_sequence 1 tcacacaggaaagtactagatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccataatactagagtcacacaggaaagtactagatgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaatactagagtcacacaggaaagtactagatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa BBa_K1725213_sequence 1 tcacacaggaaagtactagatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccataatactagagtcacacaggaaagtactagatgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaa BBa_B0032_sequence 1 tcacacaggaaag BBa_K1725219_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccataatactagagtcacacaggaaagtactagatgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaatactagagtcacacaggaaagtactagatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z