##gff-version 3
##sequence-region BBa_K1725219 1 3356
BBa_K1725219	.	engineered_region	209	3356	.	+	0	ID=BBa_K1725217;Name=BBa_K1725217
BBa_K1725219	.	engineered_region	2627	3356	.	+	0	ID=BBa_K1725211;Name=BBa_K1725211;Parent=BBa_K1725217
BBa_K1725219	.	ribosome_entry_site	2627	2639	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725211
BBa_K1725219	.	ribosome_entry_site	2633	2636	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725219	.	engineered_region	2627	2639	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725219	.	ribosome_entry_site	2627	2639	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725219	.	CDS	2646	3356	.	+	0	ID=BBa_K1725205;Name=BBa_K1725205;Parent=BBa_K1725211
BBa_K1725219	.	CDS	2646	3356	.	+	0	ID=annotation2434651;Name=LuxG;Parent=BBa_K1725205
BBa_K1725219	.	engineered_region	209	2618	.	+	0	ID=BBa_K1725213;Name=BBa_K1725213;Parent=BBa_K1725217
BBa_K1725219	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725213
BBa_K1725219	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725219	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725219	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725219	.	CDS	228	1667	.	+	0	ID=BBa_K1725202;Name=BBa_K1725202;Parent=BBa_K1725213
BBa_K1725219	.	CDS	228	1667	.	+	0	ID=annotation2434648;Name=LuxC;Parent=BBa_K1725202
BBa_K1725219	.	CDS	1695	2618	.	+	0	ID=BBa_K1725203;Name=BBa_K1725203;Parent=BBa_K1725213
BBa_K1725219	.	CDS	1695	2618	.	+	0	ID=annotation2434649;Name=LuxD;Parent=BBa_K1725203
BBa_K1725219	.	ribosome_entry_site	1676	1688	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725213
BBa_K1725219	.	ribosome_entry_site	1682	1685	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725219	.	engineered_region	1676	1688	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725219	.	ribosome_entry_site	1676	1688	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725219	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1725219	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1725219	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1725219	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1725219	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1725219	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1725219	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1725219	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_K1725219
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaataaat
gtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataa
tagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaattta
aatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatcc
gtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctc
taaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgat
tgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatcta
ttttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagt
ttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagat
atgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcgg
taaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaaga
tatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtt
tattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaa
aaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgag
aaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtg
tatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatc
cttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataa
tattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagacca
tttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttg
tcccataatactagagtcacacaggaaagtactagatgaaagatgaaagtgctttttttacgattgatcacatcatcaa
gcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatactt
attgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtca
ttcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatag
cctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgaga
atcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacat
tagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataa
tttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaa
ggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattg
atagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtatt
aagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattatt
gaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattag
aattggcttaatactagagtcacacaggaaagtactagatgattgttgatggtagagtttcaaaaatagtattagcgtc
aataagaaataatatatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtc
acgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggta
gttcgaatagagactgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttaga
tgctccccatggaaacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatca
tatataaatagcattctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagaca
gttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattga
agataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttat
tttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagt
cagaacagatgtttgccgatgcttttgcatacgtataa