LOCUS BBa_K1725220 7412 bp DNA linear 02-May-2024 DEFINITION LuxABG with pBAD and LuxCDE with R00N1 ACCESSION BBa_K1725220 VERSION BBa_K1725220.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 CDS 1238..2302 /label=LuxA CDS 1238..2302 /label=BBa_K1725200 RBS 2311..2323 /label=RBS-3\Weak RBS 2317..2320 /label=RBS misc_feature 2311..2323 /label=BBa_B0032 RBS 2311..2323 /label=BBa_B0032 CDS 2330..3310 /label=LuxB CDS 2330..3310 /label=BBa_K1725201 misc_feature 1219..3310 /label=BBa_K1725212 RBS 3319..3331 /label=RBS-3\Weak RBS 3325..3328 /label=RBS misc_feature 3319..3331 /label=BBa_B0032 RBS 3319..3331 /label=BBa_B0032 CDS 3338..4048 /label=LuxG CDS 3338..4048 /label=BBa_K1725205 misc_feature 3319..4048 /label=BBa_K1725211 misc_feature 1219..4048 /label=BBa_K1725216 misc_feature 1..4048 /label=BBa_K1725218 CDS 4057..4144 /label=end of LacI coding region (inactive) misc_feature 4057..4256 /label=BBa_R0010 misc_feature 4145..4182 /label=CAP binding site promoter 4193..4198 /label=-35 promoter 4217..4222 /label=-10 misc_feature 4222..4256 /label=LacI binding site misc_feature 4229..4229 /label=start promoter 4057..4256 /label=BBa_R0010 RBS 4265..4277 /label=RBS-3\Weak RBS 4271..4274 /label=RBS misc_feature 4265..4277 /label=BBa_B0032 RBS 4265..4277 /label=BBa_B0032 CDS 4284..5723 /label=LuxC CDS 4284..5723 /label=BBa_K1725202 RBS 5732..5744 /label=RBS-3\Weak RBS 5738..5741 /label=RBS misc_feature 5732..5744 /label=BBa_B0032 RBS 5732..5744 /label=BBa_B0032 CDS 5751..6674 /label=LuxD CDS 5751..6674 /label=BBa_K1725203 misc_feature 4265..6674 /label=BBa_K1725213 RBS 6683..6695 /label=RBS-3\Weak RBS 6689..6692 /label=RBS misc_feature 6683..6695 /label=BBa_B0032 RBS 6683..6695 /label=BBa_B0032 CDS 6702..7412 /label=LuxG CDS 6702..7412 /label=BBa_K1725205 misc_feature 6683..7412 /label=BBa_K1725211 misc_feature 4265..7412 /label=BBa_K1725217 misc_feature 4057..7412 /label=BBa_K1725219 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg aagtttggaa atatttgttt 1261 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg 1321 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga 1381 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac 1441 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga 1501 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg 1561 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac 1621 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag 1681 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc 1741 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc 1801 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa 1861 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat 1921 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa 1981 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg 2041 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa 2101 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga 2161 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc 2221 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc 2281 tttcttaaaa gaacctaaat aatactagag tcacacagga aagtactaga tgaaatttgg 2341 attatttttt ctaaactttc agaaagatgg aataacatct gaagaaacgt tggataatat 2401 ggtaaagact gtcacgttaa ttgattcaac taaatatcat tttaatactg cctttgttaa 2461 tgaacatcac ttttcaaaaa atggtattgt tggagcacct attaccgcag ctggtttttt 2521 attagggtta acaaataaat tacatattgg ttcattaaat caagtaatta ccacccatca 2581 ccctgtacgt gtagcagaag aagccagttt attagatcaa atgtcagagg gacgcttcat 2641 tcttggtttt agtgactgcg aaagtgattt cgaaatggaa ttttttagac gtcatatctc 2701 atcaaggcaa caacaatttg aagcatgcta tgaaataatt aatgacgcat taactacagg 2761 ttattgtcat ccccaaaacg acttttatga ttttccaaag gtttcaatta atccacactg 2821 ttacagtgag aatggaccta agcaatatgt atccgctaca tcaaaagaag tcgtcatgtg 2881 ggcagcgaaa aaggcactgc ctttaacatt taagtgggag gataatttag aaaccaaaga 2941 acgctatgca attctatata ataaaacagc acaacaatat ggtattgata tttcggatgt 3001 tgatcatcaa ttaactgtaa ttgcgaactt aaatgctgat agaagtacgg ctcaagaaga 3061 agtgagagaa tacttaaaag actatatcac tgaaacttac cctcaaatgg acagagatga 3121 aaaaattaac tgcattattg aagagaatgc agttgggtct catgatgact attatgaatc 3181 gacaaaatta gcagtggaaa aaacagggtc taaaaatatt ttattatcct ttgaatcaat 3241 gtccgatatt aaagatgtaa aagatattat tgatatgttg aaccaaaaaa tcgaaatgaa 3301 tttaccataa tactagagtc acacaggaaa gtactagatg attgttgatg gtagagtttc 3361 aaaaatagta ttagcgtcaa taagaaataa tatatataag gtatttatta ctgtaaattc 3421 accaataaag ttcatcgctg gacaatttgt aatggtcacg attaatggga aaaaatgccc 3481 tttttcaatt gcgaattgcc cgacaaaaaa ttacgaaata gaattgcata ttggtagttc 3541 gaatagagac tgctcattgg atattatcga atattttgtc gatgctcttg ttgaggaagt 3601 cgcaattgag ttagatgctc cccatggaaa cgcttggtta cggtctgaaa gtaataaccc 3661 attgctatta attgcgggag gtacaggttt atcatatata aatagcattc taacaaattg 3721 cttaaatagg aatatacctc aagatattta tctttactgg ggagtaaaag acagttctct 3781 tttgtatgaa gatgaagagt tactaaactt atcactaaac aacaaaaact ttcattatat 3841 tcctgttatt gaagataaaa gtgaagaatg gatagggaga aaaggcactg ttcttgatgc 3901 tgtcatggaa gattttactg atcttactta ttttgatatt tatgtttgtg gacccttcat 3961 gatggctaaa acagcaaaag aaaaattaat tgaagagaaa aaagcaaagt cagaacagat 4021 gtttgccgat gcttttgcat acgtataata ctagagcaat acgcaaaccg cctctccccg 4081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 4141 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 4201 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact 4261 agagtcacac aggaaagtac tagatgaata aatgtattcc aatgataatt aatggaatga 4321 ttcaagattt tgataattat gcatataaag aagttaaact aaataatgat aatagagtaa 4381 aattatctgt cattactgaa agttcagttt caaaaacatt aaatatcaaa gatagaatta 4441 atctaaattt aaatcagatt gtgaattttt tatataccgt tggtcaacga tggaaaagtg 4501 aagaatataa tcggcgacga acctatatcc gtgagttaaa aacatatctt ggttattctg 4561 atgaaatggc aagattagaa gcgaattgga ttgcaatgtt attgtgctct aaaagtgctt 4621 tgtatgacat tgttaattat gatttgggct ctatacacgt attagatgaa tggcttccac 4681 gtggtgattg ctatgttaaa gcacaaccga aaggtgtttc tgttcacttg ttagctggta 4741 atgttccatt atcaggagtg acatctattt tgcgtgctat tttaacaaaa aatgagtgca 4801 ttattaaaac ttcgtcttca gatcctttta ctgcaaacgc tttagtttcc agttttattg 4861 atgttaatgc agaccatcca ataaccaaat caatgtctgt tatgtattgg ccgcatgatg 4921 aagatatgac tctatctcaa agaataatga atcatgccga cgtggttatt gcttggggtg 4981 gagacgaggc gattaaatgg gcggtaaaat attcaccacc gcatgtcgat attctgaaat 5041 ttggaccaaa gaaaagctta agtattattg aagctcctaa agatatagaa gccgcagcaa 5101 tgggggttgc tcatgatatt tgtttctatg accagcaagc ctgcttctct actcaagacg 5161 tttattatat aggagataat ttacctttat ttttaaatga acttgaaaaa cagctagatc 5221 gatacgcgaa aattttacca aaaggttcaa atagttttga tgaaaaagcg gcgtttactc 5281 ttactgaaaa agaaagtcta tttgctggat atgaagtgag aaagggagat aagcaagctt 5341 ggttaatagt cgtatcacct acaaatagct ttggaaatca accgctatca cgaagtgtgt 5401 atgttcatca agtatctgat attaaagaga taattccttt tgttaataaa aatagaacac 5461 aaactgtttc tatttatcct tgggaagcgt cattaaaata tcgagataaa ttagcaagaa 5521 gtggagttga aagaattgtt gaatcaggca tgaataatat tttcagagtt ggaggggctc 5581 atgattcatt atctcctctc cagtacctag ttaggtttgt atcgcatgag agaccattta 5641 attatacgac aaaagatgtt gcggttgaaa tcgaacaaac acgttactta gaggaagata 5701 agtttttagt ttttgtccca taatactaga gtcacacagg aaagtactag atgaaagatg 5761 aaagtgcttt ttttacgatt gatcacatca tcaagcttga taatggtcag tctatccgag 5821 tttgggaaac actccctaaa aagaacgtac cagagaaaaa acatacaata cttattgctt 5881 cgggttttgc tagaagaatg gatcattttg caggtcttgc tgagtattta tctactaacg 5941 gttttcatgt cattcgctac gattctttgc atcatgttgg attaagcagt ggatgtataa 6001 atgaatttac gatgtcgatt ggaaaaaata gcctgcttac agtcgtagat tggcttacag 6061 atcatggtgt cgaacgaata gggctgattg ctgctagttt gtcagcgaga atcgcctatg 6121 aggtagtaaa taaaattaaa ttatcatttt taattacggc cgtaggtgtc gttaatctta 6181 gagatacatt agaaaaagca ttggagtatg actatttgca attacctatt tcagagttac 6241 cagaagatct tgactttgaa ggtcataatt taggatcgga ggtctttgtt acagattgct 6301 ttaaacataa ttgggacacg ttagactcga cacttaatag tgttaaagga ttagcgattc 6361 catttattgc ttttactgca aacgatgata gttgggtaaa gcaaagtgaa gttatagagc 6421 tcattgatag cattgaatct agtaattgta agctctattc gctaattgga agttcacatg 6481 atcttgggga aaatttggtt gtattaagaa atttttatca atcagtgacg aaggcagcct 6541 tagcattaga tgatggttta ttggatttag agatagacat tattgaacct cgatttgagg 6601 acgttacaag tattactgtt aaggagcgta gattaaaaaa tgaaattgaa aatgaattat 6661 tagaattggc ttaatactag agtcacacag gaaagtacta gatgattgtt gatggtagag 6721 tttcaaaaat agtattagcg tcaataagaa ataatatata taaggtattt attactgtaa 6781 attcaccaat aaagttcatc gctggacaat ttgtaatggt cacgattaat gggaaaaaat 6841 gccctttttc aattgcgaat tgcccgacaa aaaattacga aatagaattg catattggta 6901 gttcgaatag agactgctca ttggatatta tcgaatattt tgtcgatgct cttgttgagg 6961 aagtcgcaat tgagttagat gctccccatg gaaacgcttg gttacggtct gaaagtaata 7021 acccattgct attaattgcg ggaggtacag gtttatcata tataaatagc attctaacaa 7081 attgcttaaa taggaatata cctcaagata tttatcttta ctggggagta aaagacagtt 7141 ctcttttgta tgaagatgaa gagttactaa acttatcact aaacaacaaa aactttcatt 7201 atattcctgt tattgaagat aaaagtgaag aatggatagg gagaaaaggc actgttcttg 7261 atgctgtcat ggaagatttt actgatctta cttattttga tatttatgtt tgtggaccct 7321 tcatgatggc taaaacagca aaagaaaaat taattgaaga gaaaaaagca aagtcagaac 7381 agatgtttgc cgatgctttt gcatacgtat aa //