##gff-version 3
##sequence-region BBa_K1725220 1 7412
BBa_K1725220	.	engineered_region	1	4048	.	+	0	ID=BBa_K1725218;Name=BBa_K1725218
BBa_K1725220	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_K1725218
BBa_K1725220	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K1725220	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K1725220	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K1725220	.	engineered_region	1219	4048	.	+	0	ID=BBa_K1725216;Name=BBa_K1725216;Parent=BBa_K1725218
BBa_K1725220	.	engineered_region	1219	3310	.	+	0	ID=BBa_K1725212;Name=BBa_K1725212;Parent=BBa_K1725216
BBa_K1725220	.	CDS	2330	3310	.	+	0	ID=BBa_K1725201;Name=BBa_K1725201;Parent=BBa_K1725212
BBa_K1725220	.	CDS	2330	3310	.	+	0	ID=annotation2434647;Name=LuxB;Parent=BBa_K1725201
BBa_K1725220	.	CDS	1238	2302	.	+	0	ID=BBa_K1725200;Name=BBa_K1725200;Parent=BBa_K1725212
BBa_K1725220	.	CDS	1238	2302	.	+	0	ID=annotation2434636;Name=LuxA;Parent=BBa_K1725200
BBa_K1725220	.	ribosome_entry_site	2311	2323	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725212
BBa_K1725220	.	engineered_region	2311	2323	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	2317	2320	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	2311	2323	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	1219	1231	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725212
BBa_K1725220	.	engineered_region	1219	1231	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	1225	1228	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	1219	1231	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725220	.	engineered_region	3319	4048	.	+	0	ID=BBa_K1725211;Name=BBa_K1725211;Parent=BBa_K1725216
BBa_K1725220	.	ribosome_entry_site	3319	3331	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725211
BBa_K1725220	.	engineered_region	3319	3331	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	3325	3328	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	3319	3331	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725220	.	CDS	3338	4048	.	+	0	ID=BBa_K1725205;Name=BBa_K1725205;Parent=BBa_K1725211
BBa_K1725220	.	CDS	3338	4048	.	+	0	ID=annotation2434651;Name=LuxG;Parent=BBa_K1725205
BBa_K1725220	.	engineered_region	4057	7412	.	+	0	ID=BBa_K1725219;Name=BBa_K1725219
BBa_K1725220	.	engineered_region	4265	7412	.	+	0	ID=BBa_K1725217;Name=BBa_K1725217;Parent=BBa_K1725219
BBa_K1725220	.	engineered_region	6683	7412	.	+	0	ID=BBa_K1725211;Name=BBa_K1725211;Parent=BBa_K1725217
BBa_K1725220	.	ribosome_entry_site	6683	6695	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725211
BBa_K1725220	.	engineered_region	6683	6695	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	6689	6692	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	6683	6695	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725220	.	CDS	6702	7412	.	+	0	ID=BBa_K1725205;Name=BBa_K1725205;Parent=BBa_K1725211
BBa_K1725220	.	CDS	6702	7412	.	+	0	ID=annotation2434651;Name=LuxG;Parent=BBa_K1725205
BBa_K1725220	.	engineered_region	4265	6674	.	+	0	ID=BBa_K1725213;Name=BBa_K1725213;Parent=BBa_K1725217
BBa_K1725220	.	ribosome_entry_site	4265	4277	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725213
BBa_K1725220	.	engineered_region	4265	4277	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	4271	4274	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	4265	4277	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725220	.	CDS	5751	6674	.	+	0	ID=BBa_K1725203;Name=BBa_K1725203;Parent=BBa_K1725213
BBa_K1725220	.	CDS	5751	6674	.	+	0	ID=annotation2434649;Name=LuxD;Parent=BBa_K1725203
BBa_K1725220	.	CDS	4284	5723	.	+	0	ID=BBa_K1725202;Name=BBa_K1725202;Parent=BBa_K1725213
BBa_K1725220	.	CDS	4284	5723	.	+	0	ID=annotation2434648;Name=LuxC;Parent=BBa_K1725202
BBa_K1725220	.	ribosome_entry_site	5732	5744	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1725213
BBa_K1725220	.	engineered_region	5732	5744	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	5738	5741	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1725220	.	ribosome_entry_site	5732	5744	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1725220	.	promoter	4057	4256	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1725219
BBa_K1725220	.	non_covalent_binding_site	4145	4182	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1725220	.	start_codon	4229	4229	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1725220	.	non_covalent_binding_site	4222	4256	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1725220	.	engineered_region	4057	4256	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1725220	.	promoter	4217	4222	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1725220	.	CDS	4057	4144	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1725220	.	promoter	4193	4198	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1725220
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatgaagtttggaaatatttgtttttcg
tatcaaccaccaggtgaaactcataagcaagtaatggatcgctttgttcggcttggtatcgcctcagaagaggtagggt
ttgatacatattggaccttagaacatcattttacagagtttggtcttacgggaaatttatttgttgctgcggctaacct
gttaggaagaactaaaacattaaatgttggcactatgggggttgttattccgacagcacacccagttcgacagttagaa
gacgttttattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcgagggctataccataaagattttc
gagtatttggtgttgatatggaagagtctcgagcaattactcaaaatttctaccagatgataatggaaagcttacagac
aggaaccattagctctgatagtgattacattcaatttcctaaggttgatgtatatcccaaagtgtactcaaaaaatgta
ccaacctgtatgactgctgagtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttcttagttgga
ttattggtactaatgaaaaaaaagcacagatggaactctataatgaaattgcgacagaatatggtcatgatatatctaa
aatagatcattgtatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatgtttgtcgggagtttctg
aaaaattggtatgactcatatgtaaatgcgaccaatatctttaatgatagcaatcaaactcgtggttatgattatcata
aaggtcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtgttgattatagcaatggtattaaccc
tgtaggcactcctgagcagtgtattgaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatgcgga
tttgaagctaatggaactgaagatgaaataattgcttccatgcgacgctttatgacacaagtcgctcctttcttaaaag
aacctaaataatactagagtcacacaggaaagtactagatgaaatttggattattttttctaaactttcagaaagatgg
aataacatctgaagaaacgttggataatatggtaaagactgtcacgttaattgattcaactaaatatcattttaatact
gcctttgttaatgaacatcacttttcaaaaaatggtattgttggagcacctattaccgcagctggttttttattagggt
taacaaataaattacatattggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcagaagaagccag
tttattagatcaaatgtcagagggacgcttcattcttggttttagtgactgcgaaagtgatttcgaaatggaatttttt
agacgtcatatctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacgcattaactacaggttatt
gtcatccccaaaacgacttttatgattttccaaaggtttcaattaatccacactgttacagtgagaatggacctaagca
atatgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcactgcctttaacatttaagtgggaggat
aatttagaaaccaaagaacgctatgcaattctatataataaaacagcacaacaatatggtattgatatttcggatgttg
atcatcaattaactgtaattgcgaacttaaatgctgatagaagtacggctcaagaagaagtgagagaatacttaaaaga
ctatatcactgaaacttaccctcaaatggacagagatgaaaaaattaactgcattattgaagagaatgcagttgggtct
catgatgactattatgaatcgacaaaattagcagtggaaaaaacagggtctaaaaatattttattatcctttgaatcaa
tgtccgatattaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaatgaatttaccataatactagag
tcacacaggaaagtactagatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataatatatat
aaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaat
gccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagagactgctc
attggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatggaaacgct
tggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagcattctaa
caaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgtatgaaga
tgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaagaatgg
atagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatgtttgtg
gacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtttgccga
tgcttttgcatacgtataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg
gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccc
caggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtc
acacaggaaagtactagatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcata
taaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaat
atcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaag
aatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagc
gaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgta
ttagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggta
atgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttc
agatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtct
gttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggg
gtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaag
cttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccag
caagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagc
tagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaaga
aagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagcttt
ggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaa
atagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttga
aagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtaccta
gttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttact
tagaggaagataagtttttagtttttgtcccataatactagagtcacacaggaaagtactagatgaaagatgaaagtgc
tttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaac
gtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagt
atttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatga
atttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaataggg
ctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccg
taggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttacc
agaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacg
ttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaa
agcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcaca
tgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggt
ttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaa
aaaatgaaattgaaaatgaattattagaattggcttaatactagagtcacacaggaaagtactagatgattgttgatgg
tagagtttcaaaaatagtattagcgtcaataagaaataatatatataaggtatttattactgtaaattcaccaataaag
ttcatcgctggacaatttgtaatggtcacgattaatgggaaaaaatgccctttttcaattgcgaattgcccgacaaaaa
attacgaaatagaattgcatattggtagttcgaatagagactgctcattggatattatcgaatattttgtcgatgctct
tgttgaggaagtcgcaattgagttagatgctccccatggaaacgcttggttacggtctgaaagtaataacccattgcta
ttaattgcgggaggtacaggtttatcatatataaatagcattctaacaaattgcttaaataggaatatacctcaagata
tttatctttactggggagtaaaagacagttctcttttgtatgaagatgaagagttactaaacttatcactaaacaacaa
aaactttcattatattcctgttattgaagataaaagtgaagaatggatagggagaaaaggcactgttcttgatgctgtc
atggaagattttactgatcttacttattttgatatttatgtttgtggacccttcatgatggctaaaacagcaaaagaaa
aattaattgaagagaaaaaagcaaagtcagaacagatgtttgccgatgcttttgcatacgtataa