LOCUS BBa_K1725224 6892 bp DNA linear 16-Dec-2024 DEFINITION pBAD Lux ABCDE ACCESSION BBa_K1725224 VERSION BBa_K1725224.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 CDS 1238..2302 /label=LuxA CDS 1238..2302 /label=BBa_K1725200 RBS 2311..2323 /label=RBS-3\Weak RBS 2317..2320 /label=RBS misc_feature 2311..2323 /label=BBa_B0032 RBS 2311..2323 /label=BBa_B0032 CDS 2330..3310 /label=LuxB CDS 2330..3310 /label=BBa_K1725201 misc_feature 1219..3310 /label=BBa_K1725212 misc_feature 1..3310 /label=BBa_K1725214 RBS 3319..3331 /label=RBS-3\Weak RBS 3325..3328 /label=RBS misc_feature 3319..3331 /label=BBa_B0032 RBS 3319..3331 /label=BBa_B0032 CDS 3338..4777 /label=LuxC CDS 3338..4777 /label=BBa_K1725202 RBS 4786..4798 /label=RBS-3\Weak RBS 4792..4795 /label=RBS misc_feature 4786..4798 /label=BBa_B0032 RBS 4786..4798 /label=BBa_B0032 CDS 4805..5728 /label=LuxD CDS 4805..5728 /label=BBa_K1725203 misc_feature 3319..5728 /label=BBa_K1725213 RBS 5737..5749 /label=RBS-3\Weak RBS 5743..5746 /label=RBS misc_feature 5737..5749 /label=BBa_B0032 RBS 5737..5749 /label=BBa_B0032 CDS 5756..6892 /label=LuxE CDS 5756..6892 /label=BBa_K1725204 misc_feature 5737..6892 /label=BBa_K1725210 misc_feature 3319..6892 /label=BBa_K1725222 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg aagtttggaa atatttgttt 1261 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg 1321 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga 1381 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac 1441 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga 1501 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg 1561 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac 1621 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag 1681 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc 1741 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc 1801 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa 1861 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat 1921 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa 1981 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg 2041 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa 2101 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga 2161 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc 2221 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc 2281 tttcttaaaa gaacctaaat aatactagag tcacacagga aagtactaga tgaaatttgg 2341 attatttttt ctaaactttc agaaagatgg aataacatct gaagaaacgt tggataatat 2401 ggtaaagact gtcacgttaa ttgattcaac taaatatcat tttaatactg cctttgttaa 2461 tgaacatcac ttttcaaaaa atggtattgt tggagcacct attaccgcag ctggtttttt 2521 attagggtta acaaataaat tacatattgg ttcattaaat caagtaatta ccacccatca 2581 ccctgtacgt gtagcagaag aagccagttt attagatcaa atgtcagagg gacgcttcat 2641 tcttggtttt agtgactgcg aaagtgattt cgaaatggaa ttttttagac gtcatatctc 2701 atcaaggcaa caacaatttg aagcatgcta tgaaataatt aatgacgcat taactacagg 2761 ttattgtcat ccccaaaacg acttttatga ttttccaaag gtttcaatta atccacactg 2821 ttacagtgag aatggaccta agcaatatgt atccgctaca tcaaaagaag tcgtcatgtg 2881 ggcagcgaaa aaggcactgc ctttaacatt taagtgggag gataatttag aaaccaaaga 2941 acgctatgca attctatata ataaaacagc acaacaatat ggtattgata tttcggatgt 3001 tgatcatcaa ttaactgtaa ttgcgaactt aaatgctgat agaagtacgg ctcaagaaga 3061 agtgagagaa tacttaaaag actatatcac tgaaacttac cctcaaatgg acagagatga 3121 aaaaattaac tgcattattg aagagaatgc agttgggtct catgatgact attatgaatc 3181 gacaaaatta gcagtggaaa aaacagggtc taaaaatatt ttattatcct ttgaatcaat 3241 gtccgatatt aaagatgtaa aagatattat tgatatgttg aaccaaaaaa tcgaaatgaa 3301 tttaccataa tactagagtc acacaggaaa gtactagatg aataaatgta ttccaatgat 3361 aattaatgga atgattcaag attttgataa ttatgcatat aaagaagtta aactaaataa 3421 tgataataga gtaaaattat ctgtcattac tgaaagttca gtttcaaaaa cattaaatat 3481 caaagataga attaatctaa atttaaatca gattgtgaat tttttatata ccgttggtca 3541 acgatggaaa agtgaagaat ataatcggcg acgaacctat atccgtgagt taaaaacata 3601 tcttggttat tctgatgaaa tggcaagatt agaagcgaat tggattgcaa tgttattgtg 3661 ctctaaaagt gctttgtatg acattgttaa ttatgatttg ggctctatac acgtattaga 3721 tgaatggctt ccacgtggtg attgctatgt taaagcacaa ccgaaaggtg tttctgttca 3781 cttgttagct ggtaatgttc cattatcagg agtgacatct attttgcgtg ctattttaac 3841 aaaaaatgag tgcattatta aaacttcgtc ttcagatcct tttactgcaa acgctttagt 3901 ttccagtttt attgatgtta atgcagacca tccaataacc aaatcaatgt ctgttatgta 3961 ttggccgcat gatgaagata tgactctatc tcaaagaata atgaatcatg ccgacgtggt 4021 tattgcttgg ggtggagacg aggcgattaa atgggcggta aaatattcac caccgcatgt 4081 cgatattctg aaatttggac caaagaaaag cttaagtatt attgaagctc ctaaagatat 4141 agaagccgca gcaatggggg ttgctcatga tatttgtttc tatgaccagc aagcctgctt 4201 ctctactcaa gacgtttatt atataggaga taatttacct ttatttttaa atgaacttga 4261 aaaacagcta gatcgatacg cgaaaatttt accaaaaggt tcaaatagtt ttgatgaaaa 4321 agcggcgttt actcttactg aaaaagaaag tctatttgct ggatatgaag tgagaaaggg 4381 agataagcaa gcttggttaa tagtcgtatc acctacaaat agctttggaa atcaaccgct 4441 atcacgaagt gtgtatgttc atcaagtatc tgatattaaa gagataattc cttttgttaa 4501 taaaaataga acacaaactg tttctattta tccttgggaa gcgtcattaa aatatcgaga 4561 taaattagca agaagtggag ttgaaagaat tgttgaatca ggcatgaata atattttcag 4621 agttggaggg gctcatgatt cattatctcc tctccagtac ctagttaggt ttgtatcgca 4681 tgagagacca tttaattata cgacaaaaga tgttgcggtt gaaatcgaac aaacacgtta 4741 cttagaggaa gataagtttt tagtttttgt cccataatac tagagtcaca caggaaagta 4801 ctagatgaaa gatgaaagtg ctttttttac gattgatcac atcatcaagc ttgataatgg 4861 tcagtctatc cgagtttggg aaacactccc taaaaagaac gtaccagaga aaaaacatac 4921 aatacttatt gcttcgggtt ttgctagaag aatggatcat tttgcaggtc ttgctgagta 4981 tttatctact aacggttttc atgtcattcg ctacgattct ttgcatcatg ttggattaag 5041 cagtggatgt ataaatgaat ttacgatgtc gattggaaaa aatagcctgc ttacagtcgt 5101 agattggctt acagatcatg gtgtcgaacg aatagggctg attgctgcta gtttgtcagc 5161 gagaatcgcc tatgaggtag taaataaaat taaattatca tttttaatta cggccgtagg 5221 tgtcgttaat cttagagata cattagaaaa agcattggag tatgactatt tgcaattacc 5281 tatttcagag ttaccagaag atcttgactt tgaaggtcat aatttaggat cggaggtctt 5341 tgttacagat tgctttaaac ataattggga cacgttagac tcgacactta atagtgttaa 5401 aggattagcg attccattta ttgcttttac tgcaaacgat gatagttggg taaagcaaag 5461 tgaagttata gagctcattg atagcattga atctagtaat tgtaagctct attcgctaat 5521 tggaagttca catgatcttg gggaaaattt ggttgtatta agaaattttt atcaatcagt 5581 gacgaaggca gccttagcat tagatgatgg tttattggat ttagagatag acattattga 5641 acctcgattt gaggacgtta caagtattac tgttaaggag cgtagattaa aaaatgaaat 5701 tgaaaatgaa ttattagaat tggcttaata ctagagtcac acaggaaagt actagatgac 5761 tgtccatact gaatataaaa gaaatcaaat cattgctagt tcagaaattg atgatcttat 5821 ctttatgacg aaaccacaag agtggtcatt tgaagagcaa aaagaaatac gggataaatt 5881 agttcgtgag gctttttatt ttcactacaa tagaaatgaa gaatatagaa attattgtat 5941 caatcagcat gtgagtgata atttacacac tattgatgaa atacccgtgt ttccaacatc 6001 tgtttttaaa tataagaaat tacatactgt cacagccgag gacattgaaa attggtatac 6061 aagtagtgga actcgtggag taaaaagtca tattgcacgt gatcgtctta gcattgaacg 6121 cttgcttggt tctgtcaact tcggaatgaa atacgttgga gattggtttg agcatcaaat 6181 ggaattgata aatttaggac cagatagatt caatacaaat aatatttggt ttaaatatgt 6241 catgagtttg gtcgagttac tttatccgac tgaatttaca gttgataatg acaaaataga 6301 ttttgaaaaa acagtaaaac atctatttag aattaagaat agtaaaaaag acatttgctt 6361 aattgggcca ccattttttg tgtatctttt gtgccaatat atgaaagaaa acaatattga 6421 atttaaagga ggagatagag tacatattat tactggtgga ggatggaaat ctaatcagaa 6481 tgactcttta gatcgtgctg attttaatca attattaatg gatactttcc aactcgacaa 6541 gattaatcaa attagagata cctttaatca agttgagctt aatacttgtt tttttgaaga 6601 tgaatttcaa agaaaacatg ttccaccgtg ggtatatgct cgggctcttg atcctgaaac 6661 cttgaaaccc gtagcagatg gtgagatcgg gttgttaagt tatatggacg cctcatcaac 6721 tgcttaccct gcttttattg ttactgatga tatcggtatt gtaaaagaaa ttagagaacc 6781 agatccttac ccaggggtaa ctgttgagat tgttcggcgc ttaaatacac gtgcgcaaaa 6841 aggatgcgcg ctctctatgg ctaatgtcat acaaaagaat atcaaggatt aa //