BBa_K1725080 1 R0011-N Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site 2015-08-10T11:00:00Z 2016-07-23T11:23:04Z From BBa_R0011. Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be: repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]). induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range Extra Nhe1 site added, for ease of checking for BioBrick with a digest false false _2146_ 27285 27284 9 true BBa_R0011 with Nhe1 site upstream of promoter (after prefix) false Mhairi Davidson annotation2455111 1 PLlac 0-1 promoter range2455111 1 7 61 annotation2455109 1 NheI site range2455109 1 1 6 BBa_K1725301 1 BBa_K1725301 Member of the RBS library - derived from BBa_B0032 2015-09-10T11:00:00Z 2015-09-18T02:50:20Z - Parts K1725225-K1725256 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR. false false _2146_ 27362 27362 9 false - false Vilija Lomeikaite annotation2476133 1 RBS range2476133 1 1 13 BBa_K1725307 1 BBa_K1725307 Member of the RBS library - derived from BBa_B0032 2015-09-10T11:00:00Z 2015-09-19T03:57:06Z Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR. false false _2146_ 27362 27362 9 false false Vilija Lomeikaite BBa_K1725351 1 BBa_K1725351 K1725080.<i>luxCDE</i> - bright 2015-09-16T11:00:00Z 2016-07-23T11:25:12Z . . false false _2146_ 27285 27284 9 true . false Mhairi Davidson component2465899 1 BBa_K1725307 component2465905 1 BBa_K1725301 component2465902 1 BBa_K1725302 component2465898 1 BBa_K1725080 component2465907 1 BBa_K1725204 component2465901 1 BBa_K1725202 component2465904 1 BBa_K1725203 annotation2465907 1 BBa_K1725204 range2465907 1 2507 3643 annotation2465902 1 BBa_K1725302 range2465902 1 1537 1549 annotation2465901 1 BBa_K1725202 range2465901 1 89 1528 annotation2465898 1 BBa_K1725080 range2465898 1 1 61 annotation2465899 1 BBa_K1725307 range2465899 1 70 82 annotation2465904 1 BBa_K1725203 range2465904 1 1556 2479 annotation2465905 1 BBa_K1725301 range2465905 1 2488 2500 BBa_K1725203 1 luxD LuxD 2015-08-03T11:00:00Z 2015-09-18T11:34:37Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434649 1 LuxD range2434649 1 1 924 BBa_K1725204 1 luxE LuxE 2015-08-03T11:00:00Z 2015-09-18T11:35:13Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434650 1 LuxE range2434650 1 1 1137 BBa_K1725202 1 luxC LuxC 2015-08-03T11:00:00Z 2015-09-18T11:34:09Z Test Test false false _2146_ 20827 27303 9 false Test false Charlotte Flynn annotation2434648 1 LuxC range2434648 1 1 1440 BBa_K1725302 1 BBa_K1725302 Member of the RBS library - derived from BBa_B0032 2015-09-10T11:00:00Z 2015-09-18T09:57:25Z - Parts K1725225-K1725256 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR. false false _2146_ 27362 27362 9 false - false Vilija Lomeikaite BBa_K1725202_sequence 1 atgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccataa BBa_K1725204_sequence 1 atgactgtccatactgaatataaaagaaatcaaatcattgctagttcagaaattgatgatcttatctttatgacgaaaccacaagagtggtcatttgaagagcaaaaagaaatacgggataaattagttcgtgaggctttttattttcactacaatagaaatgaagaatatagaaattattgtatcaatcagcatgtgagtgataatttacacactattgatgaaatacccgtgtttccaacatctgtttttaaatataagaaattacatactgtcacagccgaggacattgaaaattggtatacaagtagtggaactcgtggagtaaaaagtcatattgcacgtgatcgtcttagcattgaacgcttgcttggttctgtcaacttcggaatgaaatacgttggagattggtttgagcatcaaatggaattgataaatttaggaccagatagattcaatacaaataatatttggtttaaatatgtcatgagtttggtcgagttactttatccgactgaatttacagttgataatgacaaaatagattttgaaaaaacagtaaaacatctatttagaattaagaatagtaaaaaagacatttgcttaattgggccaccattttttgtgtatcttttgtgccaatatatgaaagaaaacaatattgaatttaaaggaggagatagagtacatattattactggtggaggatggaaatctaatcagaatgactctttagatcgtgctgattttaatcaattattaatggatactttccaactcgacaagattaatcaaattagagatacctttaatcaagttgagcttaatacttgtttttttgaagatgaatttcaaagaaaacatgttccaccgtgggtatatgctcgggctcttgatcctgaaaccttgaaacccgtagcagatggtgagatcgggttgttaagttatatggacgcctcatcaactgcttaccctgcttttattgttactgatgatatcggtattgtaaaagaaattagagaaccagatccttacccaggggtaactgttgagattgttcggcgcttaaatacacgtgcgcaaaaaggatgcgcgctctctatggctaatgtcatacaaaagaatatcaaggattaa BBa_K1725302_sequence 1 tcacacaggagag BBa_K1725203_sequence 1 atgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaa BBa_K1725351_sequence 1 gctagcaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacatgagggtactagatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtattattgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgcttctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgatacgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctatttgctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaaccgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacacaaactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgttgaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttgtatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaagataagtttttagtttttgtcccataatactagagtcacacaggagagtactagatgaaagatgaaagtgctttttttacgattgatcacatcatcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaatacttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcatgtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaaatagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagcgagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagatacattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtcataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgttaaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaatactagagtcacacaggagggtactagatgactgtccatactgaatataaaagaaatcaaatcattgctagttcagaaattgatgatcttatctttatgacgaaaccacaagagtggtcatttgaagagcaaaaagaaatacgggataaattagttcgtgaggctttttattttcactacaatagaaatgaagaatatagaaattattgtatcaatcagcatgtgagtgataatttacacactattgatgaaatacccgtgtttccaacatctgtttttaaatataagaaattacatactgtcacagccgaggacattgaaaattggtatacaagtagtggaactcgtggagtaaaaagtcatattgcacgtgatcgtcttagcattgaacgcttgcttggttctgtcaacttcggaatgaaatacgttggagattggtttgagcatcaaatggaattgataaatttaggaccagatagattcaatacaaataatatttggtttaaatatgtcatgagtttggtcgagttactttatccgactgaatttacagttgataatgacaaaatagattttgaaaaaacagtaaaacatctatttagaattaagaatagtaaaaaagacatttgcttaattgggccaccattttttgtgtatcttttgtgccaatatatgaaagaaaacaatattgaatttaaaggaggagatagagtacatattattactggtggaggatggaaatctaatcagaatgactctttagatcgtgctgattttaatcaattattaatggatactttccaactcgacaagattaatcaaattagagatacctttaatcaagttgagcttaatacttgtttttttgaagatgaatttcaaagaaaacatgttccaccgtgggtatatgctcgggctcttgatcctgaaaccttgaaacccgtagcagatggtgagatcgggttgttaagttatatggacgcctcatcaactgcttaccctgcttttattgttactgatgatatcggtattgtaaaagaaattagagaaccagatccttacccaggggtaactgttgagattgttcggcgcttaaatacacgtgcgcaaaaaggatgcgcgctctctatggctaatgtcatacaaaagaatatcaaggattaa BBa_K1725080_sequence 1 gctagcaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K1725307_sequence 1 tcacacatgaggg BBa_K1725301_sequence 1 tcacacaggaggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z