LOCUS BBa_K1725352 7699 bp DNA linear 22-Nov-2024 DEFINITION pBAD/araC.luxABG.R0011N.luxCDE - bright ACCESSION BBa_K1725352 VERSION BBa_K1725352.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=BBa_K1725305 CDS 1238..2302 /label=LuxA CDS 1238..2302 /label=BBa_K1725200 RBS 2311..2323 /label=BBa_K1725309 CDS 2330..3310 /label=LuxB CDS 2330..3310 /label=BBa_K1725201 RBS 3319..3331 /label=BBa_K1725302 CDS 3338..4048 /label=LuxG CDS 3338..4048 /label=BBa_K1725205 misc_feature 1..4048 /label=BBa_K1725350 misc_feature 4057..4062 /label=NheI site promoter 4063..4117 /label=PLlac 0-1 promoter promoter 4057..4117 /label=BBa_K1725080 RBS 4126..4138 /label=BBa_K1725307 CDS 4145..5584 /label=LuxC CDS 4145..5584 /label=BBa_K1725202 RBS 5593..5605 /label=BBa_K1725302 CDS 5612..6535 /label=LuxD CDS 5612..6535 /label=BBa_K1725203 RBS 6544..6556 /label=RBS RBS 6544..6556 /label=BBa_K1725301 CDS 6563..7699 /label=LuxE CDS 6563..7699 /label=BBa_K1725204 misc_feature 4057..7699 /label=BBa_K1725351 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaagaag gtactagatg aagtttggaa atatttgttt 1261 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg 1321 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga 1381 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac 1441 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga 1501 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg 1561 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac 1621 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag 1681 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc 1741 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc 1801 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa 1861 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat 1921 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa 1981 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg 2041 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa 2101 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga 2161 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc 2221 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc 2281 tttcttaaaa gaacctaaat aatactagag tcacacaaaa gggtactaga tgaaatttgg 2341 attatttttt ctaaactttc agaaagatgg aataacatct gaagaaacgt tggataatat 2401 ggtaaagact gtcacgttaa ttgattcaac taaatatcat tttaatactg cctttgttaa 2461 tgaacatcac ttttcaaaaa atggtattgt tggagcacct attaccgcag ctggtttttt 2521 attagggtta acaaataaat tacatattgg ttcattaaat caagtaatta ccacccatca 2581 ccctgtacgt gtagcagaag aagccagttt attagatcaa atgtcagagg gacgcttcat 2641 tcttggtttt agtgactgcg aaagtgattt cgaaatggaa ttttttagac gtcatatctc 2701 atcaaggcaa caacaatttg aagcatgcta tgaaataatt aatgacgcat taactacagg 2761 ttattgtcat ccccaaaacg acttttatga ttttccaaag gtttcaatta atccacactg 2821 ttacagtgag aatggaccta agcaatatgt atccgctaca tcaaaagaag tcgtcatgtg 2881 ggcagcgaaa aaggcactgc ctttaacatt taagtgggag gataatttag aaaccaaaga 2941 acgctatgca attctatata ataaaacagc acaacaatat ggtattgata tttcggatgt 3001 tgatcatcaa ttaactgtaa ttgcgaactt aaatgctgat agaagtacgg ctcaagaaga 3061 agtgagagaa tacttaaaag actatatcac tgaaacttac cctcaaatgg acagagatga 3121 aaaaattaac tgcattattg aagagaatgc agttgggtct catgatgact attatgaatc 3181 gacaaaatta gcagtggaaa aaacagggtc taaaaatatt ttattatcct ttgaatcaat 3241 gtccgatatt aaagatgtaa aagatattat tgatatgttg aaccaaaaaa tcgaaatgaa 3301 tttaccataa tactagagtc acacaggaga gtactagatg attgttgatg gtagagtttc 3361 aaaaatagta ttagcgtcaa taagaaataa tatatataag gtatttatta ctgtaaattc 3421 accaataaag ttcatcgctg gacaatttgt aatggtcacg attaatggga aaaaatgccc 3481 tttttcaatt gcgaattgcc cgacaaaaaa ttacgaaata gaattgcata ttggtagttc 3541 gaatagagac tgctcattgg atattatcga atattttgtc gatgctcttg ttgaggaagt 3601 cgcaattgag ttagatgctc cccatggaaa cgcttggtta cggtctgaaa gtaataaccc 3661 attgctatta attgcgggag gtacaggttt atcatatata aatagcattc taacaaattg 3721 cttaaatagg aatatacctc aagatattta tctttactgg ggagtaaaag acagttctct 3781 tttgtatgaa gatgaagagt tactaaactt atcactaaac aacaaaaact ttcattatat 3841 tcctgttatt gaagataaaa gtgaagaatg gatagggaga aaaggcactg ttcttgatgc 3901 tgtcatggaa gattttactg atcttactta ttttgatatt tatgtttgtg gacccttcat 3961 gatggctaaa acagcaaaag aaaaattaat tgaagagaaa aaagcaaagt cagaacagat 4021 gtttgccgat gcttttgcat acgtataata ctagaggcta gcaattgtga gcggataaca 4081 attgacattg tgagcggata acaagatact gagcacatac tagagtcaca catgagggta 4141 ctagatgaat aaatgtattc caatgataat taatggaatg attcaagatt ttgataatta 4201 tgcatataaa gaagttaaac taaataatga taatagagta aaattatctg tcattactga 4261 aagttcagtt tcaaaaacat taaatatcaa agatagaatt aatctaaatt taaatcagat 4321 tgtgaatttt ttatataccg ttggtcaacg atggaaaagt gaagaatata atcggcgacg 4381 aacctatatc cgtgagttaa aaacatatct tggttattct gatgaaatgg caagattaga 4441 agcgaattgg attgcaatgt tattgtgctc taaaagtgct ttgtatgaca ttgttaatta 4501 tgatttgggc tctatacacg tattagatga atggcttcca cgtggtgatt gctatgttaa 4561 agcacaaccg aaaggtgttt ctgttcactt gttagctggt aatgttccat tatcaggagt 4621 gacatctatt ttgcgtgcta ttttaacaaa aaatgagtgc attattaaaa cttcgtcttc 4681 agatcctttt actgcaaacg ctttagtttc cagttttatt gatgttaatg cagaccatcc 4741 aataaccaaa tcaatgtctg ttatgtattg gccgcatgat gaagatatga ctctatctca 4801 aagaataatg aatcatgccg acgtggttat tgcttggggt ggagacgagg cgattaaatg 4861 ggcggtaaaa tattcaccac cgcatgtcga tattctgaaa tttggaccaa agaaaagctt 4921 aagtattatt gaagctccta aagatataga agccgcagca atgggggttg ctcatgatat 4981 ttgtttctat gaccagcaag cctgcttctc tactcaagac gtttattata taggagataa 5041 tttaccttta tttttaaatg aacttgaaaa acagctagat cgatacgcga aaattttacc 5101 aaaaggttca aatagttttg atgaaaaagc ggcgtttact cttactgaaa aagaaagtct 5161 atttgctgga tatgaagtga gaaagggaga taagcaagct tggttaatag tcgtatcacc 5221 tacaaatagc tttggaaatc aaccgctatc acgaagtgtg tatgttcatc aagtatctga 5281 tattaaagag ataattcctt ttgttaataa aaatagaaca caaactgttt ctatttatcc 5341 ttgggaagcg tcattaaaat atcgagataa attagcaaga agtggagttg aaagaattgt 5401 tgaatcaggc atgaataata ttttcagagt tggaggggct catgattcat tatctcctct 5461 ccagtaccta gttaggtttg tatcgcatga gagaccattt aattatacga caaaagatgt 5521 tgcggttgaa atcgaacaaa cacgttactt agaggaagat aagtttttag tttttgtccc 5581 ataatactag agtcacacag gagagtacta gatgaaagat gaaagtgctt tttttacgat 5641 tgatcacatc atcaagcttg ataatggtca gtctatccga gtttgggaaa cactccctaa 5701 aaagaacgta ccagagaaaa aacatacaat acttattgct tcgggttttg ctagaagaat 5761 ggatcatttt gcaggtcttg ctgagtattt atctactaac ggttttcatg tcattcgcta 5821 cgattctttg catcatgttg gattaagcag tggatgtata aatgaattta cgatgtcgat 5881 tggaaaaaat agcctgctta cagtcgtaga ttggcttaca gatcatggtg tcgaacgaat 5941 agggctgatt gctgctagtt tgtcagcgag aatcgcctat gaggtagtaa ataaaattaa 6001 attatcattt ttaattacgg ccgtaggtgt cgttaatctt agagatacat tagaaaaagc 6061 attggagtat gactatttgc aattacctat ttcagagtta ccagaagatc ttgactttga 6121 aggtcataat ttaggatcgg aggtctttgt tacagattgc tttaaacata attgggacac 6181 gttagactcg acacttaata gtgttaaagg attagcgatt ccatttattg cttttactgc 6241 aaacgatgat agttgggtaa agcaaagtga agttatagag ctcattgata gcattgaatc 6301 tagtaattgt aagctctatt cgctaattgg aagttcacat gatcttgggg aaaatttggt 6361 tgtattaaga aatttttatc aatcagtgac gaaggcagcc ttagcattag atgatggttt 6421 attggattta gagatagaca ttattgaacc tcgatttgag gacgttacaa gtattactgt 6481 taaggagcgt agattaaaaa atgaaattga aaatgaatta ttagaattgg cttaatacta 6541 gagtcacaca ggagggtact agatgactgt ccatactgaa tataaaagaa atcaaatcat 6601 tgctagttca gaaattgatg atcttatctt tatgacgaaa ccacaagagt ggtcatttga 6661 agagcaaaaa gaaatacggg ataaattagt tcgtgaggct ttttattttc actacaatag 6721 aaatgaagaa tatagaaatt attgtatcaa tcagcatgtg agtgataatt tacacactat 6781 tgatgaaata cccgtgtttc caacatctgt ttttaaatat aagaaattac atactgtcac 6841 agccgaggac attgaaaatt ggtatacaag tagtggaact cgtggagtaa aaagtcatat 6901 tgcacgtgat cgtcttagca ttgaacgctt gcttggttct gtcaacttcg gaatgaaata 6961 cgttggagat tggtttgagc atcaaatgga attgataaat ttaggaccag atagattcaa 7021 tacaaataat atttggttta aatatgtcat gagtttggtc gagttacttt atccgactga 7081 atttacagtt gataatgaca aaatagattt tgaaaaaaca gtaaaacatc tatttagaat 7141 taagaatagt aaaaaagaca tttgcttaat tgggccacca ttttttgtgt atcttttgtg 7201 ccaatatatg aaagaaaaca atattgaatt taaaggagga gatagagtac atattattac 7261 tggtggagga tggaaatcta atcagaatga ctctttagat cgtgctgatt ttaatcaatt 7321 attaatggat actttccaac tcgacaagat taatcaaatt agagatacct ttaatcaagt 7381 tgagcttaat acttgttttt ttgaagatga atttcaaaga aaacatgttc caccgtgggt 7441 atatgctcgg gctcttgatc ctgaaacctt gaaacccgta gcagatggtg agatcgggtt 7501 gttaagttat atggacgcct catcaactgc ttaccctgct tttattgtta ctgatgatat 7561 cggtattgta aaagaaatta gagaaccaga tccttaccca ggggtaactg ttgagattgt 7621 tcggcgctta aatacacgtg cgcaaaaagg atgcgcgctc tctatggcta atgtcataca 7681 aaagaatatc aaggattaa //