BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_I13500 1 BBa_I13500 Screening plasmid intermediate 2005-05-30T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 Built by Josh as an intermediate in screening plasmid construction false true _11_ 0 253 6 In stock false true jkm component1505002 1 BBa_E0040 component1505000 1 BBa_B0034 annotation1505002 1 BBa_E0040 range1505002 1 19 738 annotation1505000 1 BBa_B0034 range1505000 1 1 12 BBa_K1725400 1 PlsiR PlsiR (Promoter of light and stress integrating response Regulator) 2015-09-07T11:00:00Z 2015-09-08T10:25:53Z Synechocystis sp. PCC6803 This promoter originally comes from the unicellular cyanobacterium Synechocystis sp. PCC6803. The promoter is normally off and is activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light. For the promoter to be activated all three parts must be present and the cell has to be exposed to UV-A. false false _2146_ 21308 21308 9 false PlsiR was ordered as a G-block gene fragment. false Andrey Filipov BBa_K1725401 1 PlsiR.GFP PlsiR + B0034 + GFP 2015-09-07T11:00:00Z 2015-09-08T11:23:40Z The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803. The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0034 RBS (BBa_I13500). This allows activity in response to UV-A to be measured. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A. false false _2146_ 21308 21308 9 false The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to I13500 after restriction that was followed by an Azure-A-based gel extraction. false Andrey Filipov component2446999 1 BBa_K1725400 component2447003 1 BBa_I13500 annotation2447003 1 BBa_I13500 range2447003 1 543 1280 annotation2446999 1 BBa_K1725400 range2446999 1 1 534 BBa_B0034_sequence 1 aaagaggagaaa BBa_I13500_sequence 1 aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1725401_sequence 1 cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1725400_sequence 1 cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z