BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_E5501 1 BBa_E5501 B0032.E0040 2005-05-16T11:00:00Z 2015-08-31T04:07:27Z Released HQ 2013 B0032.E0040 false true _1_ 0 61 1 In stock true false jcbraff component1489098 1 BBa_B0032 component1489101 1 BBa_E0040 annotation1489098 1 BBa_B0032 range1489098 1 1 13 annotation1489101 1 BBa_E0040 range1489101 1 20 739 BBa_K1725402 1 PlsirE5501 PlsiR + B0032 + GFP 2015-09-07T11:00:00Z 2015-09-08T11:23:05Z The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803. The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0032 RBS (BBa_E5501). This allows activity in response to UV-A to be measured. This construct is expected to result in less fluorescence than K1725401. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A. false false _2146_ 21308 21308 9 false The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to E5501 after restriction that was followed by an Azure-A-based gel extraction. false Andrey Filipov component2447004 1 BBa_K1725400 component2447009 1 BBa_E5501 annotation2447009 1 BBa_E5501 range2447009 1 543 1281 annotation2447004 1 BBa_K1725400 range2447004 1 1 534 BBa_K1725400 1 PlsiR PlsiR (Promoter of light and stress integrating response Regulator) 2015-09-07T11:00:00Z 2015-09-08T10:25:53Z Synechocystis sp. PCC6803 This promoter originally comes from the unicellular cyanobacterium Synechocystis sp. PCC6803. The promoter is normally off and is activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light. For the promoter to be activated all three parts must be present and the cell has to be exposed to UV-A. false false _2146_ 21308 21308 9 false PlsiR was ordered as a G-block gene fragment. false Andrey Filipov BBa_K1725402_sequence 1 cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0032_sequence 1 tcacacaggaaag BBa_E5501_sequence 1 tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1725400_sequence 1 cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z