BBa_K1725430
type: ComponentDefinition
persistent identity: BBa K1725430
display id: BBa_K1725430
version: 1
was generated by: iGEM to SBOL conversion
description: J23101 + RBS + UirR + B0015 + PlsiR + GFP
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T01:23:02Z
mutable provenance: UirR and Plsir come from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: myers
owned by: james
mutable description: The UV intensity response regulator coding sequence was positioned downstream of J23101, a constitutive promoter family member, and upstream of the double terminator B0015. In this part the promoter associated with UirR was also added, alongside with GFP, so that activity may be reported.
discontinued: false
dominant: false
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: J23101, UirR, and B0015 were ligated to Plsir and GFP after restriction that was followed by an Azure-A-based gel extraction.
star: false
creator: Andrey Filipov
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1725426
component: BBa_K1725401
sequence annotation: BBa_K1725426
sequence annotation: BBa_K1725401
sequence: https://synbiohub.org/public/igem/BBa_K1725430_sequence/1

BBa_K1725426
type: Component
persistent identity: component2447332
display id: component2447332
version: 1
top level: BBa_K1725430
definition: BBa_K1725426
access: public

BBa_K1725401
type: Component
persistent identity: component2447338
display id: component2447338
version: 1
top level: BBa_K1725430
definition: PlsiR.GFP
access: public

BBa_K1725426
type: SequenceAnnotation
persistent identity: annotation2447332
display id: annotation2447332
version: 1
top level: BBa_K1725430
location: https://synbiohub.org/public/igem/BBa_K1725430/annotation2447332/range2447332/1
component: BBa_K1725426

https://synbiohub.org/public/igem/BBa_K1725430/annotation2447332/range2447332/1
type: Range
persistent identity: range2447332
display id: range2447332
version: 1
top level: BBa_K1725430
direction: direction/reverse
start: 1
end: 925
orientation: inline

BBa_K1725401
type: SequenceAnnotation
persistent identity: annotation2447338
display id: annotation2447338
version: 1
top level: BBa_K1725430
location: https://synbiohub.org/public/igem/BBa_K1725430/annotation2447338/range2447338/1
component: BBa_K1725401

https://synbiohub.org/public/igem/BBa_K1725430/annotation2447338/range2447338/1
type: Range
persistent identity: range2447338
display id: range2447338
version: 1
top level: BBa_K1725430
direction: direction/reverse
start: 934
end: 2213
orientation: inline

PlsiR.GFP
type: ComponentDefinition
persistent identity: BBa K1725401
display id: BBa_K1725401
version: 1
was generated by: iGEM to SBOL conversion
description: PlsiR + B0034 + GFP
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T11:23:40Z
mutable provenance: The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: james
owned by: myers
mutable description: The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0034 RBS (BBa_I13500). This allows activity in response to UV-A to be measured. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to I13500 after restriction that was followed by an Azure-A-based gel extraction.
star: false
creator: Andrey Filipov
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1725400
component: BBa_I13500
sequence annotation: BBa_K1725400
sequence annotation: BBa_I13500
sequence: https://synbiohub.org/public/igem/BBa_K1725401_sequence/1

BBa_K1725400
type: Component
persistent identity: component2446999
display id: component2446999
version: 1
top level: PlsiR.GFP
definition: PlsiR
access: public

BBa_I13500
type: Component
persistent identity: component2447003
display id: component2447003
version: 1
top level: PlsiR.GFP
definition: BBa_I13500
access: public

BBa_K1725400
type: SequenceAnnotation
persistent identity: annotation2446999
display id: annotation2446999
version: 1
top level: PlsiR.GFP
location: https://synbiohub.org/public/igem/BBa_K1725401/annotation2446999/range2446999/1
component: BBa_K1725400

https://synbiohub.org/public/igem/BBa_K1725401/annotation2446999/range2446999/1
type: Range
persistent identity: range2446999
display id: range2446999
version: 1
top level: PlsiR.GFP
direction: direction/reverse
start: 1
end: 534
orientation: inline

BBa_I13500
type: SequenceAnnotation
persistent identity: annotation2447003
display id: annotation2447003
version: 1
top level: PlsiR.GFP
location: https://synbiohub.org/public/igem/BBa_K1725401/annotation2447003/range2447003/1
component: BBa_I13500

https://synbiohub.org/public/igem/BBa_K1725401/annotation2447003/range2447003/1
type: Range
persistent identity: range2447003
display id: range2447003
version: 1
top level: PlsiR.GFP
direction: direction/reverse
start: 543
end: 1280
orientation: inline

BBa_K1725426
type: ComponentDefinition
persistent identity: BBa K1725426
display id: BBa_K1725426
version: 1
was generated by: iGEM to SBOL conversion
description: J23101 + RBS + UirR + B0015
created: 2015-09-07T11:00:00Z
modified: 2015-09-16T07:37:24Z
mutable provenance: The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: james
owned by: myers
mutable description: The UV intensity response regulator coding sequence was positioned downstream of J23101, a constitutive promoter family member, and upstream of the double terminator B0015. This allows the part to be placed in front of other parts with weaker or non-constitutive promoters.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The regulator and J23101 were ligated to B0015 after restriction that was followed by an Azure-A-based gel extraction.
star: false
creator: Andrey Filipov
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1725422
sequence annotation: BBa_K1725422
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1725426_sequence/1

BBa_B0015
type: Component
persistent identity: component2447166
display id: component2447166
version: 1
top level: BBa_K1725426
definition: BBa_B0015
access: public

BBa_K1725422
type: Component
persistent identity: component2447159
display id: component2447159
version: 1
top level: BBa_K1725426
definition: BBa_K1725422
access: public

BBa_K1725422
type: SequenceAnnotation
persistent identity: annotation2447159
display id: annotation2447159
version: 1
top level: BBa_K1725426
location: https://synbiohub.org/public/igem/BBa_K1725426/annotation2447159/range2447159/1
component: BBa_K1725422

https://synbiohub.org/public/igem/BBa_K1725426/annotation2447159/range2447159/1
type: Range
persistent identity: range2447159
display id: range2447159
version: 1
top level: BBa_K1725426
direction: direction/reverse
start: 1
end: 788
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2447166
display id: annotation2447166
version: 1
top level: BBa_K1725426
location: https://synbiohub.org/public/igem/BBa_K1725426/annotation2447166/range2447166/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1725426/annotation2447166/range2447166/1
type: Range
persistent identity: range2447166
display id: range2447166
version: 1
top level: BBa_K1725426
direction: direction/reverse
start: 797
end: 925
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
definition: BBa_B0034
access: public

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
definition: GFP
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
type: Range
persistent identity: range1505000
display id: range1505000
version: 1
top level: BBa_I13500
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
type: Range
persistent identity: range1505002
display id: range1505002
version: 1
top level: BBa_I13500
direction: direction/forward
start: 19
end: 738
orientation: inline

PlsiR
type: ComponentDefinition
persistent identity: BBa K1725400
display id: BBa_K1725400
version: 1
was generated by: iGEM to SBOL conversion
description: PlsiR (Promoter of light and stress integrating response Regulator)
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T10:25:53Z
mutable provenance: Synechocystis sp. PCC6803
owned by: james
owned by: myers
mutable description: This promoter originally comes from the unicellular cyanobacterium Synechocystis sp. PCC6803. The promoter is normally off and is activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light. For the promoter to be activated all three parts must be present and the cell has to be exposed to UV-A.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: PlsiR was ordered as a G-block gene fragment.
star: false
creator: Andrey Filipov
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1725400_sequence/1

BBa_K1725422
type: ComponentDefinition
persistent identity: BBa K1725422
display id: BBa_K1725422
version: 1
was generated by: iGEM to SBOL conversion
description: J23101 + RBS + UirR
created: 2015-09-07T11:00:00Z
modified: 2015-09-16T07:38:10Z
mutable provenance: The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: james
owned by: myers
mutable description: The UV intensity response regulator coding sequence was placed downstream of J23101, a constitutive promoter family member.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The regulator was ligated to J23101 (which came in the form of an annealed oligo) after restriction that was followed by an Azure-A-based gel extraction.
star: false
creator: Andrey Filipov
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23101
component: BBa_K1725421
sequence annotation: BBa_J23101
sequence annotation: BBa_K1725421
sequence: https://synbiohub.org/public/igem/BBa_K1725422_sequence/1

BBa_J23101
type: Component
persistent identity: component2447031
display id: component2447031
version: 1
top level: BBa_K1725422
definition: BBa_J23101
access: public

BBa_K1725421
type: Component
persistent identity: component2447036
display id: component2447036
version: 1
top level: BBa_K1725422
definition: BBa_K1725421
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2447031
display id: annotation2447031
version: 1
top level: BBa_K1725422
location: https://synbiohub.org/public/igem/BBa_K1725422/annotation2447031/range2447031/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1725422/annotation2447031/range2447031/1
type: Range
persistent identity: range2447031
display id: range2447031
version: 1
top level: BBa_K1725422
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1725421
type: SequenceAnnotation
persistent identity: annotation2447036
display id: annotation2447036
version: 1
top level: BBa_K1725422
location: https://synbiohub.org/public/igem/BBa_K1725422/annotation2447036/range2447036/1
component: BBa_K1725421

https://synbiohub.org/public/igem/BBa_K1725422/annotation2447036/range2447036/1
type: Range
persistent identity: range2447036
display id: range2447036
version: 1
top level: BBa_K1725422
direction: direction/reverse
start: 44
end: 788
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K1725421
type: ComponentDefinition
persistent identity: BBa K1725421
display id: BBa_K1725421
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + UirR
created: 2015-09-07T11:00:00Z
modified: 2016-01-25T11:31:22Z
mutable provenance: UirR comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: james
owned by: myers
mutable description: The regulator (K1725420) that participates together with PlsiR (K1725400) and UirS (K1725410) to activate a response to UV-A light has been put downstream of the RBS B0032.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 4206
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Due to the length of the DNA sequence, the gene was amplified via PCR and the BioBrick suffix and prefix, alongside with the RBS, were added as part of the primers. The last base of the gene was changed from G to A because different stop codons can be more efficient in different species and TAA is especially efficient in E. coli.
star: false
creator: Andrey Filipov
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_K1725420
sequence annotation: BBa_B0032
sequence annotation: BBa_K1725420
sequence: https://synbiohub.org/public/igem/BBa_K1725421_sequence/1

BBa_B0032
type: Component
persistent identity: component2447020
display id: component2447020
version: 1
top level: BBa_K1725421
definition: BBa_B0032
access: public

BBa_K1725420
type: Component
persistent identity: component2447022
display id: component2447022
version: 1
top level: BBa_K1725421
definition: UirR
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2447020
display id: annotation2447020
version: 1
top level: BBa_K1725421
location: https://synbiohub.org/public/igem/BBa_K1725421/annotation2447020/range2447020/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1725421/annotation2447020/range2447020/1
type: Range
persistent identity: range2447020
display id: range2447020
version: 1
top level: BBa_K1725421
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1725420
type: SequenceAnnotation
persistent identity: annotation2447022
display id: annotation2447022
version: 1
top level: BBa_K1725421
location: https://synbiohub.org/public/igem/BBa_K1725421/annotation2447022/range2447022/1
component: BBa_K1725420

https://synbiohub.org/public/igem/BBa_K1725421/annotation2447022/range2447022/1
type: Range
persistent identity: range2447022
display id: range2447022
version: 1
top level: BBa_K1725421
direction: direction/reverse
start: 20
end: 745
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

UirR
type: ComponentDefinition
persistent identity: BBa K1725420
display id: BBa_K1725420
version: 1
was generated by: iGEM to SBOL conversion
description: UirR (UV intensity response Regulator)
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T06:52:10Z
mutable provenance: The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803
owned by: james
owned by: myers
mutable description: UirR is a protein that attaches itself to the transmembrane protein UirS (K1725410). After exposure to unidirectional UV-A, UirS releases UirR, which physically interacts with the lsiR promoter (K1725400), activating transcription. All three parts are required for a cell to respond to the UV-A stimulus.
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Due to the length of the DNA sequence, the gene was amplified via PCR and the BioBrick suffix and prefix were added as part of the primers. The last base of the gene was changed from G to A because different stop codons can be more efficient in different species and TAA is especially efficient in E. coli.
star: false
creator: Andrey Filipov
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1725420_sequence/1

https://synbiohub.org/public/igem/BBa_K1725430_sequence/1
type: Sequence
persistent identity: BBa K1725430 sequence
display id: BBa_K1725430_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725401_sequence/1
type: Sequence
persistent identity: BBa K1725401 sequence
display id: BBa_K1725401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725426_sequence/1
type: Sequence
persistent identity: BBa K1725426 sequence
display id: BBa_K1725426_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13500_sequence/1
type: Sequence
persistent identity: BBa I13500 sequence
display id: BBa_I13500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725400_sequence/1
type: Sequence
persistent identity: BBa K1725400 sequence
display id: BBa_K1725400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725422_sequence/1
type: Sequence
persistent identity: BBa K1725422 sequence
display id: BBa_K1725422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725421_sequence/1
type: Sequence
persistent identity: BBa K1725421 sequence
display id: BBa_K1725421_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725420_sequence/1
type: Sequence
persistent identity: BBa K1725420 sequence
display id: BBa_K1725420_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z