LOCUS       BBa_K1725431            2213 bp    DNA     linear       22-Dec-2024
DEFINITION  J23110 + RBS + UirR + B0015 + PlsiR + GFP
ACCESSION   BBa_K1725431
VERSION     BBa_K1725431.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..788
                     /label=BBa_K1725420
     misc_feature    44..788
                     /label=BBa_K1725421
     misc_feature    1..788
                     /label=BBa_K1725423
     stem_loop       808..851
                     /label=stem_loop
     misc_feature    797..876
                     /label=BBa_B0010
     terminator      797..876
                     /label=BBa_B0010
     stem_loop       892..911
                     /label=T7 TE
     misc_feature    918..918
                     /label=stop
     polyA_site      912..925
                     /label=polya
     misc_feature    885..925
                     /label=BBa_B0012
     terminator      885..925
                     /label=BBa_B0012
     terminator      797..925
                     /label=BBa_B0015
     misc_feature    1..925
                     /label=BBa_K1725427
     promoter        934..1467
                     /label=BBa_K1725400
     misc_feature    1480..1483
                     /label=conserved
     RBS             1476..1487
                     /label=BBa_B0034
     CDS             1494..2213
                     /label=GFP protein
     CDS             1494..2213
                     /label=BBa_E0040
     misc_feature    1476..2213
                     /label=BBa_I13500
     misc_feature    934..2213
                     /label=BBa_K1725401
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtcacaca ggaaagtact
       61 agatggtaac caaaatactg attgtagagg atgaaaggct agtggcccaa cacattgccc
      121 aattattaaa aagtgatggc tatgaaattt gtgtgatcgc cagtgatgga gcaacggcgc
      181 tgaaaaaaat tgctgagttt tatccagatc tagttttact agatattcgt attaaaggag
      241 agatcgacgg gatagaggtg gcggaacgga taaaatctct ttactccatc cccattgttt
      301 atctcacagc tttttctgat ggggaaaccc tggagcgggc ccagaaaacc aatccccagg
      361 gctatgtgat taagcctttt cggcgggaac aactattatc caccgtggcg atcgccatag
      421 ccaatcatca acagcaacga aaacctgagg aagataccct ctccacgtca acgggccatt
      481 atcgcctgca accgactttg gactatatcg aagagcatct cgaccaagga attaccgttg
      541 aatttctggc cggggcgatc ggcatgagca cagcctactt ttgtcgcttt ttccaaaagg
      601 aaatgggatg ttccccctat caatttatta tccaacaacg ggttgagcgg gccaaagcca
      661 tactactgga aagagaattg tccattagcg aagtagcttt gagatgcggc ttcagttccc
      721 acagtcaact taaccaccat tttcgtaatc tcttgggcat taccccgaaa gaatatcgta
      781 gtcgctaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      841 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      901 tcgggtgggc ctttctgcgt ttatatacta gagcagtttc caagcaagat ccgggcgaaa
      961 aaataaaatc gatgtcaaaa atcaactaga ttgtcaaaaa tcgaccagga cagggttggg
     1021 aatttttcct aggatgggac tgtgctggtg agcgacgggc tgcgaatgcc attggctcaa
     1081 atcccaacct tgccaaggat tagacctact cctacccgct tatttttagg caagttcttg
     1141 caggtttgaa tcctaaccga catttttgct tctaggcctt ggatagcgag agttaccctt
     1201 ggccaccaaa ccttggctaa gtcgatgcct tttggtaccc caactagcag caaacaccag
     1261 tcatcttcct tgaatccttc tctggtcagt cctacgggtc actgtttgga gcaatgcctg
     1321 gggcattgag catttcctcc catgtcaatc acaactgaca gttaaaacaa gggaattact
     1381 cggccctagg tctccttatc ccactgacaa cagcattaac cgaacactgt taaagtttat
     1441 ctttccaaac atgcaaagga gtttccctac tagagaaaga ggagaaatac tagatgcgta
     1501 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     1561 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1621 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     1681 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     1741 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1801 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1861 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1921 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1981 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     2041 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     2101 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     2161 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taa
//