BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K322122 1 BBa_K322122 Phycocyanobilin synthesis operon 2010-09-25T11:00:00Z 2015-05-08T01:11:59Z This is identical to K098010, deposited by Harvard 2008, except that we have transferred it to pSB1C3. Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)&#65292; used in light sensor. false false _441_ 0 6225 9 It's complicated true Features: heme oxygenase coding sequence 63 to 629; pcyA coding sequence 650 to 1396; terminator B0015 bases 1403 to 1502 (according to the K098010 annotation, but no scar!). Notes: the start sequence is not quite as K098010(made by Havard 2008). Basically just the first 5 bases are different from K098010, of which the first 5 bases are ctgat. Also base 24 is T rather than G. All of these are absolutely clear on the chromatogram. false Chris French and Richard Edward Partridge-Hicks annotation2083038 1 Terminator (B0015) range2083038 1 1403 1502 annotation2083040 1 J23110 range2083040 1 1 35 annotation2083041 1 B0032 range2083041 1 43 54 annotation2083042 1 rbs range2083042 1 630 644 annotation2083039 1 pcyA range2083039 1 650 1396 annotation2083037 1 Heme oxygenase range2083037 1 63 629 BBa_K1725410 1 BBa_K1725410 uirS 2015-09-09T11:00:00Z 2015-09-10T12:27:34Z . . false false _2146_ 27284 27284 9 false . false Mhairi Davidson BBa_K1725445 1 BBa_K1725445 J23106 + RBS + uirS + K322122 2015-09-16T11:00:00Z 2015-09-17T07:06:32Z . . false false _2146_ 27285 27285 9 false . false James Provan component2465874 1 BBa_K1725410 component2465881 1 BBa_K322122 component2465870 1 BBa_J23106 component2465872 1 BBa_B0032 annotation2465881 1 BBa_K322122 range2465881 1 2606 4136 annotation2465872 1 BBa_B0032 range2465872 1 44 56 annotation2465874 1 BBa_K1725410 range2465874 1 63 2597 annotation2465870 1 BBa_J23106 range2465870 1 1 35 BBa_J23106 1 BBa_J23106 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1725445_sequence 1 tttacggctagctcagtcctaggtatagtgctagctactagagtcacacaggaaagtactagatggcaatcaccgcatttaccctgggggattttttccaagccaacagctatataccccacggccattgctacctttggcaaactcccctggtctggctccacgtcagcgctgattttttcacggcgatcgcctactactctattcccttaactctgctctactttttgcggaagcgccaagatattccttttcctaatattattttcctgtttagtacttttattttatgctgtggcaccagtcacttttttgacattatcaccctttggtaccctatttattggatatccggtacagtcaaagcctccatggcgatcgtctctattatcactgtgtttgaattaattcaaattgtacccaatgccctaaatttaaaatcccccacggaattagctaccctcaatctggctctaaatcaagaaattaaagaaaggcaaacagcggaaatagcattgcaggagttaaacaataatcttgaaaaaagagtggaagaccgcactacccaattagcaaaaataaatcaacaattagaacaggaaattgaagataaaacccgggcaaaagaagatctcgaaaaaaataaagatcaattggcgcaattggctgctattgtcgaatcttcccaggacgcaattattagtaaaaccctagatggtaacatcaccagttggaacgagtctgcggaaagattatttggctacacagcagaagaaatgattggaagtcatatcaccaaacttattcctgaggaattaatcttggaagaagaccttattgctgaatgcatccgccaaggtcagagaattaacacctacgaaactcaaagacagcgtaaagatggtactaaaattgatgttgccctgaccatttctcccattcgagatgaacacaaaaatgtagtgggagcctccaaaattgttagggatattaccgccagattggatgtggaaaatgccctacgggaaagtcaatattttattgaaaaattagctaattattctccccagattctttatattttagaccccattgcttggaaaaatatttatgtcaattatcaatccttcgaaatcctaggctacactccagaagaatttaaaaatgggggaacggagctgttgttaaatattgtgcatcccgatgacatcccaaccctttacgaaaataagaatttttggcaaaagtcccaggaaggccaagtgttgaccactgaatatcgaatgcgccacaaaaatggttcttggcggtggctacgttctcgggaagtggtttttgctagggatgattatgggcaagttactaaggttttgggcacagcccaggatattagtgatagcaaagaacaggaacaaagactttatgagcaaggccgccgcgaatcattattaagggaaattacccagcgtatccgtcagtctttagatctgccaactatttttaacacagtagtgcaggaaattcgtcagtttttggaagcagatcgggtagttatttttcagttttcacccgactctgacttttccgttggtaatattgtggcagagtcggtattggctccatttaagccaatcattaatagtgcaattgaagaaacttgttttagtaataactatgcccaaaggtatcagcagggcagaatccaggtcattgaggatattcaccagtcccatcttaggcaatgccacattgactttcttgccaggctacaggtcagggcaaacctagtgctaccactaattaatgatgccattttgtggggcttattgtgtattcatcaatgtgacagttctcgagtttgggaacaaacagaaattgatctgctcaagcagatcactaatcagtttgaaattgccatccaacaggctactttgtacgagcaggctcaacaagaattggcttctaaaaatcagctttttgtacaattaaccaacgaattagagcaaaaaaaagtattgcttaaggaaattcatcatcgagttaaaaacaatttgcaaattatgtctagtttactttatttgcaatttagtaaggcttctccagccatccagcaattgagtgaggaataccagaatcgcattcaatccatggcattgattcatgagcagttatatcgtagtgaagacttagctaatattgattttagtcaatatcttaaaaatttgactcacaatatctgccaaagttacggctgtaatactgatagtattaaaattaaattgttggttgaacaagttaaagttcctttagagcagtccattcctttgggattaattattcaggaattagtttccaatgctttaaaacatgctttcccaacgacggaaggggaaatttcgattaaattcacttcaatgaacagtcactatagtttacaggtgtgggataacggagttggaatttccagggatatagatttggaaaatacagatagtttgggcatgcaacttatctacagcctaactgaacaactccagggagaattgcactatgaatatgtcggtggcgctcaatttgggttggaattttcactttaatactagagtttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataaggtaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_J23106_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K1725410_sequence 1 atggcaatcaccgcatttaccctgggggattttttccaagccaacagctatataccccacggccattgctacctttggcaaactcccctggtctggctccacgtcagcgctgattttttcacggcgatcgcctactactctattcccttaactctgctctactttttgcggaagcgccaagatattccttttcctaatattattttcctgtttagtacttttattttatgctgtggcaccagtcacttttttgacattatcaccctttggtaccctatttattggatatccggtacagtcaaagcctccatggcgatcgtctctattatcactgtgtttgaattaattcaaattgtacccaatgccctaaatttaaaatcccccacggaattagctaccctcaatctggctctaaatcaagaaattaaagaaaggcaaacagcggaaatagcattgcaggagttaaacaataatcttgaaaaaagagtggaagaccgcactacccaattagcaaaaataaatcaacaattagaacaggaaattgaagataaaacccgggcaaaagaagatctcgaaaaaaataaagatcaattggcgcaattggctgctattgtcgaatcttcccaggacgcaattattagtaaaaccctagatggtaacatcaccagttggaacgagtctgcggaaagattatttggctacacagcagaagaaatgattggaagtcatatcaccaaacttattcctgaggaattaatcttggaagaagaccttattgctgaatgcatccgccaaggtcagagaattaacacctacgaaactcaaagacagcgtaaagatggtactaaaattgatgttgccctgaccatttctcccattcgagatgaacacaaaaatgtagtgggagcctccaaaattgttagggatattaccgccagattggatgtggaaaatgccctacgggaaagtcaatattttattgaaaaattagctaattattctccccagattctttatattttagaccccattgcttggaaaaatatttatgtcaattatcaatccttcgaaatcctaggctacactccagaagaatttaaaaatgggggaacggagctgttgttaaatattgtgcatcccgatgacatcccaaccctttacgaaaataagaatttttggcaaaagtcccaggaaggccaagtgttgaccactgaatatcgaatgcgccacaaaaatggttcttggcggtggctacgttctcgggaagtggtttttgctagggatgattatgggcaagttactaaggttttgggcacagcccaggatattagtgatagcaaagaacaggaacaaagactttatgagcaaggccgccgcgaatcattattaagggaaattacccagcgtatccgtcagtctttagatctgccaactatttttaacacagtagtgcaggaaattcgtcagtttttggaagcagatcgggtagttatttttcagttttcacccgactctgacttttccgttggtaatattgtggcagagtcggtattggctccatttaagccaatcattaatagtgcaattgaagaaacttgttttagtaataactatgcccaaaggtatcagcagggcagaatccaggtcattgaggatattcaccagtcccatcttaggcaatgccacattgactttcttgccaggctacaggtcagggcaaacctagtgctaccactaattaatgatgccattttgtggggcttattgtgtattcatcaatgtgacagttctcgagtttgggaacaaacagaaattgatctgctcaagcagatcactaatcagtttgaaattgccatccaacaggctactttgtacgagcaggctcaacaagaattggcttctaaaaatcagctttttgtacaattaaccaacgaattagagcaaaaaaaagtattgcttaaggaaattcatcatcgagttaaaaacaatttgcaaattatgtctagtttactttatttgcaatttagtaaggcttctccagccatccagcaattgagtgaggaataccagaatcgcattcaatccatggcattgattcatgagcagttatatcgtagtgaagacttagctaatattgattttagtcaatatcttaaaaatttgactcacaatatctgccaaagttacggctgtaatactgatagtattaaaattaaattgttggttgaacaagttaaagttcctttagagcagtccattcctttgggattaattattcaggaattagtttccaatgctttaaaacatgctttcccaacgacggaaggggaaatttcgattaaattcacttcaatgaacagtcactatagtttacaggtgtgggataacggagttggaatttccagggatatagatttggaaaatacagatagtttgggcatgcaacttatctacagcctaactgaacaactccagggagaattgcactatgaatatgtcggtggcgctcaatttgggttggaattttcactttaa BBa_K322122_sequence 1 tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataaggtaccccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z