LOCUS       BBa_K1727007             555 bp    DNA     linear       25-Nov-2024
DEFINITION  T5+Lac Operator+RBS+signal peptide+Epinecidin-1+Terminater
ACCESSION   BBa_K1727007
VERSION     BBa_K1727007.1  
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label=T5 promoter
     misc_feature    16..21
                     /label=-35
     misc_feature    21..37
                     /label=Lac operator
     misc_feature    39..45
                     /label=-10
     misc_feature    53..69
                     /label=Lac operator
     RBS             92..97
                     /label=RBS
     misc_feature    112..117
                     /label=Restriction enzyme BamH I site
     CDS             118..207
                     /label=Signal peptide
     misc_feature    415..420
                     /label=Restriction enzyme Bgl II site
     CDS             211..414
                     /label=Epinecidin-1
     CDS             208..210
                     /label=Alanine cutting site
     misc_feature    461..555
                     /label=lambda t0 terminator
ORIGIN
        1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
       61 ggataacaat ttcacacaga attcattaaa gaggagaaat taaccatggg aggatccatg
      121 ggctttcgtc ataaagcggc ggcgctggcg gcgaccctgg cgctgccgct ggcgggcctg
      181 gtgggcctgg cgagcccggc gcaggcggcg atgaggtgca tcgccctctt tcttgtgttg
      241 tcgctggtgg tcctcatggc tgaacccggg gagggtttta tcttccacat cattaaagga
      301 ctctttcacg ctggcaagat gatccatgga cttgtcacca ggagacgaca tggcgtggaa
      361 gagctgcaag acctggacca acgtgccttt gaacgagaga aagcttttgc ctgaagatct
      421 catcaccatc accatcacta agcttaatta gctgagcttg gactcctgtt gatagatcca
      481 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
      541 tttattggtg agaat
//