LOCUS       BBa_K1727008             399 bp    DNA     linear       01-Apr-2025
DEFINITION  T5+Lac Operator+RBS+Signal peptide+Signiferin+Terminater
ACCESSION   BBa_K1727008
VERSION     BBa_K1727008.1  
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label=T5 promoter
     misc_feature    16..21
                     /label=-35
     misc_feature    39..45
                     /label=-10
     misc_feature    53..69
                     /label=Lac operator
     RBS             92..97
                     /label=RBS
     misc_feature    21..37
                     /label=Lac operator
     misc_feature    104..109
                     /label=Restriction enzyme Nco I site
     CDS             106..195
                     /label=Signal peptide
     CDS             196..198
                     /label=Alanine cutting site
     CDS             199..243
                     /label=Signiferin
     misc_feature    244..252
                     /label=3X stop codon
     misc_feature    253..258
                     /label=Restriction enzyme BamH I site
     misc_feature    305..399
                     /label=lambda t0 terminator
ORIGIN
        1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
       61 ggataacaat ttcacacaga attcattaaa gaggagaaat taaccatggg ctttcgtcat
      121 aaagcggcgg cgctggcggc gaccctggcg ctgccgctgg cgggcctggt gggcctggcg
      181 agcccggcgc aggcggcgat tattggccat ctgattaaaa ccgcgctggg catgctgggc
      241 ctgtaataat aaggatccag atctcatcac catcaccatc actaagctta attagctgag
      301 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
      361 acgctcggtt gccgccgggc gttttttatt ggtgagaat
//