##gff-version 3 ##sequence-region BBa_K1727008 1 399 BBa_K1727008 . sequence_feature 253 258 . + 0 ID=annotation2460073;Name=Restriction enzyme BamH I site BBa_K1727008 . operator 53 69 . + 0 ID=annotation2444151;Name=Lac operator BBa_K1727008 . sequence_feature 39 45 . + 0 ID=annotation2444150;Name=-10 BBa_K1727008 . CDS 106 195 . + 0 ID=annotation2459984;Name=Signal peptide BBa_K1727008 . stop_codon 244 252 . + 0 ID=annotation2460025;Name=3X stop codon BBa_K1727008 . operator 21 37 . + 0 ID=annotation2444153;Name=Lac operator BBa_K1727008 . ribosome_entry_site 92 97 . + 0 ID=annotation2444152;Name=RBS BBa_K1727008 . promoter 1 45 . + 0 ID=annotation2444148;Name=T5 promoter BBa_K1727008 . CDS 196 198 . + 0 ID=annotation2460008;Name=Alanine cutting site BBa_K1727008 . CDS 199 243 . + 0 ID=annotation2460009;Name=Signiferin BBa_K1727008 . sequence_feature 16 21 . + 0 ID=annotation2444149;Name=-35 BBa_K1727008 . stop_codon 305 399 . + 0 ID=annotation2460185;Name=lambda t0 terminator BBa_K1727008 . sequence_feature 104 109 . + 0 ID=annotation2459629;Name=Restriction enzyme Nco I site >BBa_K1727008 tcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacag aattcattaaagaggagaaattaaccatgggctttcgtcataaagcggcggcgctggcggcgaccctggcgctgccgct ggcgggcctggtgggcctggcgagcccggcgcaggcggcgattattggccatctgattaaaaccgcgctgggcatgctg ggcctgtaataataaggatccagatctcatcaccatcaccatcactaagcttaattagctgagcttggactcctgttga tagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtga gaat