LOCUS       BBa_K1728017            1767 bp    DNA     linear       27-Sep-2024
DEFINITION  IL1β toehold switch RNA sensor with T7 promoter & luciferase 
            reporter
ACCESSION   BBa_K1728017
VERSION     BBa_K1728017.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     RBS             63..70
                     /label=RBS
     misc_feature    78..80
                     /label=Start Codon
     promoter        24..108
                     /label=BBa_K1728001
     misc_feature    1..108
                     /label=BBa_K1728009
     CDS             115..1767
                     /label=BBa_I712019
ORIGIN
        1 taatacgact cactataggg agatttccct tcatctttga agaagaacct atcatacaga
       61 aacagaggag atatgatatg ttcttcttca acctggcggc agcgcaaata ctagatggaa
      121 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
      181 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
      241 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
      301 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
      361 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
      421 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
      481 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
      541 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
      601 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
      661 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
      721 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
      781 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
      841 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
      901 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
      961 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
     1021 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
     1081 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
     1141 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     1201 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     1261 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
     1321 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     1381 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
     1441 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
     1501 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
     1561 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     1621 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     1681 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     1741 aagaagggcg gaaagatcgc cgtgtaa
//