LOCUS       BBa_K173004             3230 bp    DNA     linear       25-Nov-2024
DEFINITION  Beta-galactosidase protein generator
ACCESSION   BBa_K173004
VERSION     BBa_K173004.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    3042..3042
                     /label=C->T
     CDS             19..3093
                     /label=lacZ
     misc_feature    19..21
                     /label=START
     misc_feature    3091..3093
                     /label=STOP
     CDS             19..3093
                     /label=BBa_I732005
     stem_loop       3113..3156
                     /label=stem_loop
     misc_feature    3102..3181
                     /label=BBa_B0010
     terminator      3102..3181
                     /label=BBa_B0010
     stem_loop       3197..3216
                     /label=T7 TE
     misc_feature    3223..3223
                     /label=stop
     polyA_site      3217..3230
                     /label=polya
     misc_feature    3190..3230
                     /label=BBa_B0012
     terminator      3190..3230
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
      301 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
      361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
      421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
      481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
      541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac
      601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
      661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
      721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
      781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
      841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
      901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
      961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
     2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
     2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
     2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
     3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc
     3061 taccattacc agttggtctg gtgtcaaaaa taatactaga gccaggcatc aaataaaacg
     3121 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3181 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
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