LOCUS       BBa_K173008             1786 bp    DNA     linear       22-Nov-2024
DEFINITION  tetR inverter - GFP
ACCESSION   BBa_K173008
VERSION     BBa_K173008.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    1..840
                     /label=BBa_P0440
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    1..902
                     /label=BBa_K173005
     RBS             911..923
                     /label=RBS-3\Weak
     RBS             917..920
                     /label=RBS
     misc_feature    911..923
                     /label=BBa_B0032
     RBS             911..923
                     /label=BBa_B0032
     CDS             930..1649
                     /label=GFP protein
     CDS             930..1649
                     /label=BBa_E0040
     stem_loop       1669..1712
                     /label=stem_loop
     misc_feature    1658..1737
                     /label=BBa_B0010
     terminator      1658..1737
                     /label=BBa_B0010
     stem_loop       1753..1772
                     /label=T7 TE
     misc_feature    1779..1779
                     /label=stop
     polyA_site      1773..1786
                     /label=polya
     misc_feature    1746..1786
                     /label=BBa_B0012
     terminator      1746..1786
                     /label=BBa_B0012
     misc_feature    911..1786
                     /label=BBa_E0240
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag
      961 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1021 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1081 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1141 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1201 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1261 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1321 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1381 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1441 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1501 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1561 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1621 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
     1681 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1741 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//