##gff-version 3
##sequence-region BBa_K173008 1 1786
BBa_K173008	.	engineered_region	911	1786	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K173008	.	terminator	1746	1786	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K173008	.	polyA_site	1773	1786	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K173008	.	stem_loop	1753	1772	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K173008	.	stop_codon	1779	1779	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K173008	.	engineered_region	1746	1786	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K173008	.	CDS	930	1649	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K173008	.	CDS	930	1649	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K173008	.	terminator	1658	1737	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K173008	.	stem_loop	1669	1712	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K173008	.	engineered_region	1658	1737	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K173008	.	ribosome_entry_site	911	923	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K173008	.	engineered_region	911	923	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K173008	.	ribosome_entry_site	917	920	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K173008	.	ribosome_entry_site	911	923	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K173008	.	engineered_region	1	902	.	+	0	ID=BBa_K173005;Name=BBa_K173005
BBa_K173008	.	engineered_region	1	840	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K173005
BBa_K173008	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K173008	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K173008	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K173008	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K173008	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K173008	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K173008	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K173008	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K173008	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K173008	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K173008	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K173008	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K173008	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K173008	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K173008	.	promoter	849	902	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K173005
BBa_K173008	.	promoter	891	896	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K173008	.	non_covalent_binding_site	874	892	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K173008	.	promoter	868	873	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K173008	.	engineered_region	849	902	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K173008	.	non_covalent_binding_site	849	867	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K173008
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaac
ttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagaggg
tgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaaca
cttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaaga
gtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagt
caagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgga
cacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaact
tcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatgg
ccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccac
atggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata