LOCUS       BBa_K173012             2298 bp    DNA     linear       22-Dec-2024
DEFINITION  Lactose/IPTG inducible system with GFP
ACCESSION   BBa_K173012
VERSION     BBa_K173012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     terminator      1223..1351
                     /label=BBa_B0015
     misc_feature    1..1351
                     /label=BBa_K201002
     misc_feature    1362..1378
                     /label=lac O1
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1401
                     /label=lac O1
     promoter        1402..1407
                     /label=-10
     misc_feature    1360..1413
                     /label=BBa_R0011
     promoter        1360..1413
                     /label=BBa_R0011
     misc_feature    1..1414
                     /label=BBa_K173010
     RBS             1423..1435
                     /label=RBS-3\Weak
     RBS             1429..1432
                     /label=RBS
     misc_feature    1423..1435
                     /label=BBa_B0032
     RBS             1423..1435
                     /label=BBa_B0032
     CDS             1442..2161
                     /label=GFP protein
     CDS             1442..2161
                     /label=BBa_E0040
     stem_loop       2181..2224
                     /label=stem_loop
     misc_feature    2170..2249
                     /label=BBa_B0010
     terminator      2170..2249
                     /label=BBa_B0010
     stem_loop       2265..2284
                     /label=T7 TE
     misc_feature    2291..2291
                     /label=stop
     polyA_site      2285..2298
                     /label=polya
     misc_feature    2258..2298
                     /label=BBa_B0012
     terminator      2258..2298
                     /label=BBa_B0012
     misc_feature    1423..2298
                     /label=BBa_E0240
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt
     1381 gacattgtga gcggataaca agatactgag cacatactag agtcacacag gaaagtacta
     1441 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1501 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1561 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1621 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1681 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1741 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1801 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1861 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1921 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     1981 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     2041 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     2101 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     2161 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2221 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2281 ggcctttctg cgtttata
//