LOCUS BBa_K173015 2854 bp DNA linear 26-Dec-2024 DEFINITION HSL inducible lysis device ACCESSION BBa_K173015 VERSION BBa_K173015.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 CDS 1086..1742 /label=T4 holin CDS 1070..1745 /label=BBa_K112805 CDS 1770..2264 /label=T4 Endolysin CDS 1754..2267 /label=BBa_K112806 stem_loop 2287..2330 /label=stem_loop misc_feature 2276..2355 /label=BBa_B0010 terminator 2276..2355 /label=BBa_B0010 stem_loop 2371..2390 /label=T7 TE misc_feature 2397..2397 /label=stop polyA_site 2391..2404 /label=polya misc_feature 2364..2404 /label=BBa_B0012 terminator 2364..2404 /label=BBa_B0012 promoter 2413..2447 /label=BBa_J23116 CDS 2470..2763 /label=T4 antiholin CDS 2456..2766 /label=BBa_K112807 stem_loop 2786..2829 /label=stem_loop misc_feature 2775..2854 /label=BBa_B0010 terminator 2775..2854 /label=BBa_B0010 misc_feature 1070..2854 /label=BBa_K112808 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagc ttaaaaggag 1081 ggtctatggc agcacctaga atatcatttt cgccctctga tattctattt ggtgttctcg 1141 atcgcttgtt caaagataac gctaccggga aggttcttgc ttcccgggta gctgtcgtaa 1201 ttcttttgtt tataatggcg attgtttggt ataggggaga tagtttcttt gagtactata 1261 agcaatcaaa gtatgaaaca tacagtgaaa ttattgaaaa ggaaagaact gcacgctttg 1321 aatctgtcgc cctggaacaa ctccagatag ttcatatatc atctgaggca gactttagtg 1381 cggtgtattc tttccgccct aaaaacttaa actattttgt tgatattata gcatacgaag 1441 gaaaattacc ttcaacaata agtgaaaaat cacttggagg atatcctgtt gataaaacta 1501 tggatgaata tacagttcat ttaaatggac gtcattatta ttccaactca aaatttgctt 1561 ttttaccaac taaaaagcct actcccgaaa taaactacat gtacagttgt ccatatttta 1621 atttggataa tatctatgct ggaacgataa ccatgtactg gtatagaaat gatcatataa 1681 gtaatgaccg ccttgaatca atatgtgctc aggcggccag aatattagga agggctaaat 1741 aattatacta gagatactta ggaggtatta tgaatatatt tgaaatgtta cgtatagatg 1801 aaggtcttag acttaaaatc tataaagaca cagaaggcta ttacactatt ggcatcggtc 1861 atttgcttac aaaaagtcca tcacttaatg ctgctaaatc tgaattagat aaagctattg 1921 ggcgtaattg caatggtgta attacaaaag atgaggctga aaaactcttt aatcaggatg 1981 ttgatgctgc tgttcgcgga atcctgagaa atgctaaatt aaaaccggtt tatgattctc 2041 ttgatgcggt tcgtcgctgt gcattgatta atatggtttt ccaaatggga gaaaccggtg 2101 tggcaggatt tactaactct ttacgtatgc ttcaacaaaa acgctgggat gaagcagcag 2161 ttaacttagc taaaagtaga tggtataatc aaacacctaa tcgcgcaaaa cgagtcatta 2221 caacgtttag aactggcact tgggacgcgt ataaaaatct ataaagctac tagagccagg 2281 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2341 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2401 tatatactag agttgacagc tagctcagtc ctagggacta tgctagctac tagaggatag 2461 gaggccttta tggccttaaa agcaacagca ctttttgcca tgctaggatt gtcatttgtt 2521 ttatctccat cgattgaagc gaatgtcgat cctcattttg ataaatttat ggaatctggt 2581 attaggcacg tttatatgct ttttgaaaat aaaagcgtag aatcgtctga acaattctat 2641 agttttatga gaacgaccta taaaaatgac ccgtgctctt ctgattttga atgtatagag 2701 cgaggcgcgg agatggcaca atcatacgct agaattatga acattaaatt ggagactgaa 2761 tgaaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2821 tcgttttatc tgttgtttgt cggtgaacgc tctc //