BBa_K173018
1
BBa_K173018
pdc protein generator
2009-10-15T11:00:00Z
2015-05-08T01:10:57Z
a
A
false
false
_281_
0
2583
9
It's complicated
true
a
false
Lorenzo Pasotti, Susanna Zucca, Paolo Magni
component2044270
1
BBa_B0010
component2044272
1
BBa_B0012
component2044267
1
BBa_B0030
component2044269
1
BBa_K173016
annotation2044270
1
BBa_B0010
range2044270
1
1740
1819
annotation2044269
1
BBa_K173016
range2044269
1
22
1731
annotation2044267
1
BBa_B0030
range2044267
1
1
15
annotation2044272
1
BBa_B0012
range2044272
1
1828
1868
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K173016
1
pdc
pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)
2009-10-14T11:00:00Z
2015-05-08T01:10:57Z
a
a
false
false
_281_
0
2583
9
Not in stock
true
a
false
Lorenzo Pasotti, Paolo Magni
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K173016_sequence
1
atgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K173018_sequence
1
attaaagaggagaaatactagatgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z