LOCUS BBa_K173022 4121 bp DNA linear 16-Feb-2025 DEFINITION adhB and pdc inducible system (HSL) ACCESSION BBa_K173022 VERSION BBa_K173022.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..2245 /label=BBa_K173017 RBS 2254..2268 /label=RBS-1\Strong RBS 2261..2265 /label=RBS misc_feature 2254..2268 /label=BBa_B0030 RBS 2254..2268 /label=BBa_B0030 CDS 2275..3984 /label=BBa_K173016 stem_loop 4004..4047 /label=stem_loop misc_feature 3993..4072 /label=BBa_B0010 terminator 3993..4072 /label=BBa_B0010 stem_loop 4088..4107 /label=T7 TE misc_feature 4114..4114 /label=stop polyA_site 4108..4121 /label=polya misc_feature 4081..4121 /label=BBa_B0012 terminator 4081..4121 /label=BBa_B0012 misc_feature 1070..4121 /label=BBa_K173020 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atggcatcaa gcacctttta tatcccgttt gttaatgaaa tgggcgaggg 1141 ttctctggaa aaagcgatca aagacctgaa tggtagtggg ttcaaaaatg ccctgattgt 1201 gtcagacgcc ttcatgaata aaagtggcgt ggtgaaacag gttgctgatc tgctgaaagc 1261 acagggtatc aatagcgccg tgtatgacgg tgttatgccg aatccgaccg ttacagccgt 1321 actggaggga ctgaaaatcc tgaaagacaa caacagcgac ttcgtgattt ctctgggtgg 1381 tggttctcca catgattgtg ccaaagccat tgccctggtg gctacaaatg gaggtgaagt 1441 gaaagattat gaggggatcg acaaaagcaa aaaaccggca ctgcctctga tgagcatcaa 1501 taccacagcg ggtacagcat ctgagatgac tcgtttctgt attatcaccg acgaggttcg 1561 ccatgtgaaa atggccatcg tggatcgtca tgttacaccg atggttagcg tgaatgatcc 1621 tctgctgatg gtgggtatgc ctaaagggct gactgccgct acaggaatgg acgccctgac 1681 tcatgccttc gaagcctatt ctagcaccgc cgctactcct attaccgacg cttgtgccct 1741 gaaagctgcc tctatgattg ccaaaaatct gaaaaccgct tgtgacaacg gcaaagacat 1801 gcctgctcgt gaggccatgg cctatgccca atttctggcc ggtatggcgt ttaataacgc 1861 ctctctgggc tatgttcatg ctatggctca ccaactggga ggctattata acctgccaca 1921 cggcgtctgt aatgctgtgc tgctgcctca tgttctggcc tataatgcca gcgtggttgc 1981 tggtcgcctg aaagatgttg gagtggctat gggtctggat attgccaatc tgggggacaa 2041 agagggtgct gaagcgacaa ttcaagccgt ccgtgacctg gctgcttcta tcggaatccc 2101 ggcaaacctg acagaactgg gcgccaaaaa agaagatgtg cctctgctgg ctgatcatgc 2161 tctgaaagac gcttgtgctc tgactaatcc tcgtcaggga gatcagaaag aagtcgagga 2221 gctgtttctg agtgcctttt gataatacta gagattaaag aggagaaata ctagatgtct 2281 tataccgtgg ggacctatct ggcagaacgc ctggttcaaa ttgggctgaa acaccacttc 2341 gctgttgccg gtgattataa cctggtgctg ctggacaatc tgctgctgaa taaaaacatg 2401 gagcaagtct attgctgtaa cgaactgaac tgtggcttta gcgctgaagg ctatgcccgt 2461 gccaaaggtg ccgccgctgc tgttgtaacc tatagtgtgg gtgccctgag tgctttcgat 2521 gctattggag gggcttatgc tgaaaacctg ccggtaatcc tgatttctgg tgccccgaac 2581 aataacgatc atgccgccgg tcatgtgctg catcatgctc tgggtaaaac cgactatcat 2641 tatcagctgg aaatggccaa aaacattact gccgctgccg aggcgattta tactccggaa 2701 gaggctccgg ccaaaattga tcatgtcatc aaaaccgcac tgcgtgagaa aaaaccggtc 2761 tatctggaaa ttgcttgtaa cattgcctca atgccgtgtg ccgctcctgg tccggctagt 2821 gccctgttta acgatgaagc ttcggatgaa gcaagcctga atgctgccgt tgaagaaacg 2881 ctgaaattca ttgccaaccg tgacaaagtc gctgtactgg tgggttctaa actgcgcgct 2941 gctggtgctg aggaagccgc tgtaaaattc gctgacgccc tgggtggagc tgtagcaacc 3001 atggctgccg ccaaatcctt ctttcctgag gagaatcctc attatatcgg cacgagctgg 3061 ggcgaggttt catatccagg ggtggagaaa accatgaaag aggcagacgc cgttatcgct 3121 ctggctcctg tgttcaatga ctattccacc accgggtgga ctgatattcc tgacccgaaa 3181 aaactggtgc tggctgaacc tcgttctgtt gtggtgaatg gcatccgttt tccgagcgtt 3241 cacctgaaag actatctgac tcgtctggcc caaaaagtgt ccaaaaaaac tggcgctctg 3301 gacttcttta aaagtctgaa tgccggggaa ctgaaaaaag ctgctccggc cgatccttct 3361 gctcctctgg ttaatgccga gattgctcgt caagttgaag ctctgctgac tccgaatacc 3421 accgttatcg ccgaaactgg tgattcctgg ttcaatgccc agcgtatgaa actgccgaat 3481 ggtgctcgtg ttgagtatga gatgcaatgg ggccatattg gttggtctgt gcctgctgct 3541 tttggttatg ccgttggtgc tcctgaacgt cgtaacatcc tgatggtagg ggatggatca 3601 tttcaactga ccgcccagga agttgctcaa atggttcgtc tgaaactgcc tgtgatcatc 3661 tttctgatta acaactatgg ctatacgatc gaggtcatga ttcacgatgg tccgtataac 3721 aacattaaaa actgggacta tgctggtctg atggaggtgt tcaatggtaa tggcgggtat 3781 gattctggag ccgggaaagg cctgaaagcg aaaaccgggg gtgagctggc tgaggctatt 3841 aaagttgccc tggccaatac agatgggcct accctgatcg aatgctttat tgggcgtgag 3901 gactgtacag aagaactggt aaaatggggg aaacgtgttg ccgctgctaa cagccgtaaa 3961 ccggtgaata aactgctgta ataatactag agccaggcat caaataaaac gaaaggctca 4021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 4081 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //