##gff-version 3
##sequence-region BBa_K173022 1 4121
BBa_K173022	.	engineered_region	1070	4121	.	+	0	ID=BBa_K173020;Name=BBa_K173020
BBa_K173022	.	ribosome_entry_site	2254	2268	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K173020
BBa_K173022	.	engineered_region	2254	2268	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K173022	.	ribosome_entry_site	2261	2265	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K173022	.	ribosome_entry_site	2254	2268	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K173022	.	CDS	2275	3984	.	+	0	ID=BBa_K173016;Name=BBa_K173016;Parent=BBa_K173020
BBa_K173022	.	terminator	3993	4072	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K173020
BBa_K173022	.	stem_loop	4004	4047	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K173022	.	engineered_region	3993	4072	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K173022	.	terminator	4081	4121	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K173020
BBa_K173022	.	polyA_site	4108	4121	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K173022	.	stem_loop	4088	4107	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K173022	.	stop_codon	4114	4114	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K173022	.	engineered_region	4081	4121	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K173022	.	ribosome_entry_site	1070	1084	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K173020
BBa_K173022	.	engineered_region	1070	1084	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K173022	.	ribosome_entry_site	1077	1081	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K173022	.	ribosome_entry_site	1070	1084	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K173022	.	CDS	1091	2245	.	+	0	ID=BBa_K173017;Name=BBa_K173017;Parent=BBa_K173020
BBa_K173022	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_K173022	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K173022	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K173022	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K173022	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K173022	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K173022	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K173022	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K173022	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K173022	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K173022	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K173022	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K173022	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K173022	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K173022	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K173022	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K173022	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K173022	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K173022	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K173022	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K173022	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K173022	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K173022	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K173022	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K173022	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K173022	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K173022	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K173022	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K173022	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K173022	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K173022	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_K173022
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatggcatcaagcacct
tttatatcccgtttgttaatgaaatgggcgagggttctctggaaaaagcgatcaaagacctgaatggtagtgggttcaa
aaatgccctgattgtgtcagacgccttcatgaataaaagtggcgtggtgaaacaggttgctgatctgctgaaagcacag
ggtatcaatagcgccgtgtatgacggtgttatgccgaatccgaccgttacagccgtactggagggactgaaaatcctga
aagacaacaacagcgacttcgtgatttctctgggtggtggttctccacatgattgtgccaaagccattgccctggtggc
tacaaatggaggtgaagtgaaagattatgaggggatcgacaaaagcaaaaaaccggcactgcctctgatgagcatcaat
accacagcgggtacagcatctgagatgactcgtttctgtattatcaccgacgaggttcgccatgtgaaaatggccatcg
tggatcgtcatgttacaccgatggttagcgtgaatgatcctctgctgatggtgggtatgcctaaagggctgactgccgc
tacaggaatggacgccctgactcatgccttcgaagcctattctagcaccgccgctactcctattaccgacgcttgtgcc
ctgaaagctgcctctatgattgccaaaaatctgaaaaccgcttgtgacaacggcaaagacatgcctgctcgtgaggcca
tggcctatgcccaatttctggccggtatggcgtttaataacgcctctctgggctatgttcatgctatggctcaccaact
gggaggctattataacctgccacacggcgtctgtaatgctgtgctgctgcctcatgttctggcctataatgccagcgtg
gttgctggtcgcctgaaagatgttggagtggctatgggtctggatattgccaatctgggggacaaagagggtgctgaag
cgacaattcaagccgtccgtgacctggctgcttctatcggaatcccggcaaacctgacagaactgggcgccaaaaaaga
agatgtgcctctgctggctgatcatgctctgaaagacgcttgtgctctgactaatcctcgtcagggagatcagaaagaa
gtcgaggagctgtttctgagtgccttttgataatactagagattaaagaggagaaatactagatgtcttataccgtggg
gacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctg
ctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaag
gctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattgg
aggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtg
ctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcga
tttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatct
ggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcg
gatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgg
gttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccat
ggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggg
gtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtgga
ctgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgt
tcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctg
aatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttg
aagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgcc
gaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgtt
ggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatgg
ttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtcc
gtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagcc
gggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggccta
ccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaa
cagccgtaaaccggtgaataaactgctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata