LOCUS       BBa_K173024             2382 bp    DNA     linear       26-Nov-2024
DEFINITION  adhB inducible system (HSL)
ACCESSION   BBa_K173024
VERSION     BBa_K173024.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1084
                     /label=RBS-1\Strong
     RBS             1077..1081
                     /label=RBS
     misc_feature    1070..1084
                     /label=BBa_B0030
     RBS             1070..1084
                     /label=BBa_B0030
     CDS             1091..2245
                     /label=BBa_K173017
     stem_loop       2265..2308
                     /label=stem_loop
     misc_feature    2254..2333
                     /label=BBa_B0010
     terminator      2254..2333
                     /label=BBa_B0010
     stem_loop       2349..2368
                     /label=T7 TE
     misc_feature    2375..2375
                     /label=stop
     polyA_site      2369..2382
                     /label=polya
     misc_feature    2342..2382
                     /label=BBa_B0012
     terminator      2342..2382
                     /label=BBa_B0012
     misc_feature    1070..2382
                     /label=BBa_K173019
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga
     1081 gaaatactag atggcatcaa gcacctttta tatcccgttt gttaatgaaa tgggcgaggg
     1141 ttctctggaa aaagcgatca aagacctgaa tggtagtggg ttcaaaaatg ccctgattgt
     1201 gtcagacgcc ttcatgaata aaagtggcgt ggtgaaacag gttgctgatc tgctgaaagc
     1261 acagggtatc aatagcgccg tgtatgacgg tgttatgccg aatccgaccg ttacagccgt
     1321 actggaggga ctgaaaatcc tgaaagacaa caacagcgac ttcgtgattt ctctgggtgg
     1381 tggttctcca catgattgtg ccaaagccat tgccctggtg gctacaaatg gaggtgaagt
     1441 gaaagattat gaggggatcg acaaaagcaa aaaaccggca ctgcctctga tgagcatcaa
     1501 taccacagcg ggtacagcat ctgagatgac tcgtttctgt attatcaccg acgaggttcg
     1561 ccatgtgaaa atggccatcg tggatcgtca tgttacaccg atggttagcg tgaatgatcc
     1621 tctgctgatg gtgggtatgc ctaaagggct gactgccgct acaggaatgg acgccctgac
     1681 tcatgccttc gaagcctatt ctagcaccgc cgctactcct attaccgacg cttgtgccct
     1741 gaaagctgcc tctatgattg ccaaaaatct gaaaaccgct tgtgacaacg gcaaagacat
     1801 gcctgctcgt gaggccatgg cctatgccca atttctggcc ggtatggcgt ttaataacgc
     1861 ctctctgggc tatgttcatg ctatggctca ccaactggga ggctattata acctgccaca
     1921 cggcgtctgt aatgctgtgc tgctgcctca tgttctggcc tataatgcca gcgtggttgc
     1981 tggtcgcctg aaagatgttg gagtggctat gggtctggat attgccaatc tgggggacaa
     2041 agagggtgct gaagcgacaa ttcaagccgt ccgtgacctg gctgcttcta tcggaatccc
     2101 ggcaaacctg acagaactgg gcgccaaaaa agaagatgtg cctctgctgg ctgatcatgc
     2161 tctgaaagac gcttgtgctc tgactaatcc tcgtcaggga gatcagaaag aagtcgagga
     2221 gctgtttctg agtgcctttt gataatacta gagccaggca tcaaataaaa cgaaaggctc
     2281 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2341 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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