BBa_K1731000 1 Trg-EnvZ Constitutive Trg-EnvZ Chimera 2015-09-13T11:00:00Z 2015-09-16T02:00:58Z Synthetic and codon optimized for E. coli expression The Chimera Trg-EnvZ is a two component system that constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the cytosolic kinase domain of osmosensor EnvZ . Trg is activated by periplasmic binding proteins, and transduces the message to EnvZ to phosphorylate OmpR, a transcription factor which binds to the OmpC promoter driving transcription of whatever gene that is downstream. false false _2153_ 25879 25311 9 false Some restriction sites were removed without altering the open reading frame. false Alejandro Rodriguez-Gama, Lizbeth Airais Bola??os Castro annotation2468684 1 BBa_B0015 range2468684 1 1560 1688 annotation2468682 1 BBa_B0034 range2468682 1 45 56 annotation2468681 1 BBa_J23101 range2468681 1 1 35 annotation2468683 1 TrgZ range2468683 1 63 1550 BBa_K1731000_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagtaaagaggagaaatactagatgaacaccacccccagccagagactaggcttcctgcaccacatcagactggtgcccctgttcgcctgcatcctgggcggcatcctggtgctgttcgccctgagcagcgccctggcgggctacttcctgtggcaggccgacagagaccagagagacgtgaccgccgagatcgagatcagaaccggcctggccaacagcagcgacttcctgagaagcgccagaatcaacatgatccaggcgggcgccgccagcagaatcgccgagatggaggccatgaaaagaaacatagcccaggccgagagcgagatcaagcagagccagcagggctacagagcctaccagaacagacccgtgaagacccccgccgacgaggccctggacaccgagctgaaccagagattccaggcctacatcaccggcatgcagcccatgctgaagtacgccaagaacggcatgttcgaggccatcatcaaccacgagagcgagcagatcagacccctggacaacgcctacaccgacatcctgaacaaggccgtgaagatcagaagcaccagagccaaccagctggccgagctggcccaccagagaaccagactaggcggcatgttcatgatcggcgccttcgtgctggccctggtgatgaccctgatcaccttcatggtgctgagaagaatcgtgatcagacccctccagcacgccgcccagagaatcgagaagatcgccagcggcgacctgaccatgaacgacgagcccgcgggcagaaacgagatcggcagactgagcagacacctccagcagcacatggccgcgggcgtgaagcagctggccgacgacagaaccctgctgatggcgggcgtgagccacgacctgagaacccccctgaccagaatcagactggccaccgagatgatgagcgagcaggacggctacctggccgagagcatcaacaaggacatcgaggagtgcaacgccatcatcgagcagttcatcgactacctgagaaccggccaggagatgccaatggagatggccgatttaaatgcggttctgggcgaggttatcgcagccgaaagtggttatgaacgtgagatcgagacggcgctgtatcctggtagcatcgaggtcaaaatgcacccacttagcatcaaacgtgcggtggccaacatggtggtaaatgcggctcgttacggtaacggctggatcaaagtatctagtgggaccgagcctaaccgcgcgtggttccaggtcgaagacgacggcccagggatagccccggaacagcgtaagcatctctttcagccatttgtgcgcggagatagtgcccgcaccatttcgggaaccggcttaggcctggctattgtgcagagaatcgtagataaccacaatgggatgctggaactcggtacgagtgagcgtggcggtcttagtattcgcgcctggttgccggtgccagtcacgagagctcagggtactacgaaagaaggttgatactagagcccaggcatcaaataaaacgaaaggctcagtcgaaagactgcgcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactagctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z