LOCUS       BBa_K1732000             794 bp    DNA     linear       06-May-2024
DEFINITION  J23100-PelB-GlucHCO-B0015
ACCESSION   BBa_K1732000
VERSION     BBa_K1732000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..127
                     /label=PelB
     misc_feature    62..64
                     /label=Start
     misc_feature    62..127
                     /label=BBa_K208004
     misc_feature    136..638
                     /label=GLucCO
     misc_feature    639..657
                     /label=6X-His
     CDS             136..657
                     /label=BBa_K1732027
     stem_loop       677..720
                     /label=stem_loop
     misc_feature    666..745
                     /label=BBa_B0010
     terminator      666..745
                     /label=BBa_B0010
     stem_loop       761..780
                     /label=T7 TE
     misc_feature    787..787
                     /label=stop
     polyA_site      781..794
                     /label=polya
     misc_feature    754..794
                     /label=BBa_B0012
     terminator      754..794
                     /label=BBa_B0012
     terminator      666..794
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaatac ctgctgccga ccgctgctgc tggtctgctg ctcctcgctg cccagccggc
      121 gatggcctac tagagaaacc gacggagaac aatgaagact ttaatatcgt ggcggtggca
      181 tcgaatttcg cgactaccga cctggacgca gaccggggta aacttccggg caaaaaactt
      241 cctttggaag tactgaaaga aatggaagcg aacgcacgta aagccgggtg tacccgtggt
      301 tgcttaatct gcctcagtca cattaagtgc acaccgaaga tgaaaaagtt cattcctggc
      361 cggtgccata cctatgaagg tgataaagaa agtgcccagg gcggcattgg tgaagccatt
      421 gtagacattc cggaaatccc aggtttcaaa gacttagaac caatggaaca gttcatcgcg
      481 caagtggacc tgtgcgtgga ttgcacgacg ggttgtctta aagggctggc caatgtccag
      541 tgctcagatc tgctgaagaa atggctgcct caacgctgcg ctacgtttgc gagcaagatt
      601 cagggccagg tggacaagat taagggtgca ggcggtgacc atcatcacca ccatcactac
      661 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      721 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      781 tttctgcgtt tata
//