BBa_K1732001
1
BBa_K1732001
J23100-CDcel-GlucCO-B0015
2015-09-02T11:00:00Z
2015-09-18T09:27:02Z
Provided by the Bruchez lab at Carnegie Mellon University.
J23100 Promoter with a RBS (BBa_S04423), CDcel domain, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia luciferase to be constitutively transcribed and translated. Luminescence can be expressed by reacting the luciferase product with coelenterazine.
true
false
_2154_
27195
27195
9
false
The sequence is codon optimized for E.coli.
The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location.
false
Donna Lee
annotation2443061
1
6XHIS
range2443061
1
1708
1725
annotation2443059
1
CDcel
range2443059
1
154
1188
annotation2443060
1
Gaussia princeps luciferase
range2443060
1
1204
1707
annotation2443063
1
B0015 terminator
range2443063
1
1738
1866
annotation2443057
1
J23100 RBS
range2443057
1
43
54
annotation2443056
1
J23100 RBS
range2443056
1
2
36
BBa_K1732001_sequence
1
attgacggctagctcagtcctaggtacagtgctagctactagaaagaggagaaatactagatggagggtaatacccgtgaggataactttaaacatctgctgggcaatgataacgttaaacgtccgtcagaagcgggcgcccttcagctgcaagaagtggatggccagatgaccctggtcgatcaacatggcgaaaagatccaactccgcggtatgagcacccacgggttacagtggttcccggaaattctgaacgataatgcgtacaaagctctggctaacgactgggaatctaatatgattcgccttgcgatgtatgtcggagaaaacgggtatgcgtcaaatcctgaattgattaagtctcgcgttattaaaggtattgatttagcgattgaaaatgatatgtatgttatcgtggattggcatgtccatgctccgggcgacccgcgcgatccagtatatgctggtgcggaagattttttccgcgatatcgcagcgttatatccgaataacccgcatatcatttacgaactggctaatgagccgtcttccaataacaatggcggcgcaggcattccgaacaacgaggaggggtggaacgcagtcaaagaatatgccgacccaatcgttgagatgttgcgggacagcggaaatgcagatgacaacatcatcattgtgggcagcccgaactggagtcagcgcccagatttagctgctgataatccaatcgatgatcaccacaccatgtataccgtgcacttctataccggctcgcatgcggcttcaacagaaagctacccaccagagacccccaactctgagcgcggcaacgtgatgagtaacactcgttacgcgctggagaacggcgttgccgtatttgctaccgaatggggcaccagtcaggcgaacggtgacggcggcccttattttgatgaggcagacgtgtggatcgagttccttaatgaaaataacatttcatgggcgaattggtccctgaccaacaagaacgaagtgagtggcgcgtttactccattcgagctgggcaagtcaaatgcaacctctctggatccgggtccggatcaggtgtgggtaccggaagagttgagcctgtctggagaatacgtccgcgctcgtattaagggggttaattatgaaccgattgatcgcactaaatacacgaaagttctcggaggtggcgggtctaaaccgacggagaacaatgaagactttaatatcgtggcggtggcatcgaatttcgcgactaccgacctggacgcagaccggggtaaacttccgggcaaaaaacttcctttggaagtactgaaagaaatggaagcgaacgcacgtaaagccgggtgtacccgtggttgcttaatctgcctcagtcacattaagtgcacaccgaagatgaaaaagttcattcctggccggtgccatacctatgaaggtgataaagaaagtgcccagggcggcattggtgaagccattgtagacattccggaaatcccaggtttcaaagacttagaaccaatggaacagttcatcgcgcaagtggacctgtgcgtggattgcacgacgggttgtcttaaagggctggccaatgtccagtgctcagatctgctgaagaaatggctgcctcaacgctgcgctacgtttgcgagcaagattcagggccaggtggacaagattaagggtgcaggcggtgaccatcatcaccaccatcactaataatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z