BBa_K1732001 1 BBa_K1732001 J23100-CDcel-GlucCO-B0015 2015-09-02T11:00:00Z 2015-09-18T09:27:02Z Provided by the Bruchez lab at Carnegie Mellon University. J23100 Promoter with a RBS (BBa_S04423), CDcel domain, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia luciferase to be constitutively transcribed and translated. Luminescence can be expressed by reacting the luciferase product with coelenterazine. true false _2154_ 27195 27195 9 false The sequence is codon optimized for E.coli. The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location. false Donna Lee annotation2443061 1 6XHIS range2443061 1 1708 1725 annotation2443059 1 CDcel range2443059 1 154 1188 annotation2443060 1 Gaussia princeps luciferase range2443060 1 1204 1707 annotation2443063 1 B0015 terminator range2443063 1 1738 1866 annotation2443057 1 J23100 RBS range2443057 1 43 54 annotation2443056 1 J23100 RBS range2443056 1 2 36 BBa_K1732001_sequence 1 attgacggctagctcagtcctaggtacagtgctagctactagaaagaggagaaatactagatggagggtaatacccgtgaggataactttaaacatctgctgggcaatgataacgttaaacgtccgtcagaagcgggcgcccttcagctgcaagaagtggatggccagatgaccctggtcgatcaacatggcgaaaagatccaactccgcggtatgagcacccacgggttacagtggttcccggaaattctgaacgataatgcgtacaaagctctggctaacgactgggaatctaatatgattcgccttgcgatgtatgtcggagaaaacgggtatgcgtcaaatcctgaattgattaagtctcgcgttattaaaggtattgatttagcgattgaaaatgatatgtatgttatcgtggattggcatgtccatgctccgggcgacccgcgcgatccagtatatgctggtgcggaagattttttccgcgatatcgcagcgttatatccgaataacccgcatatcatttacgaactggctaatgagccgtcttccaataacaatggcggcgcaggcattccgaacaacgaggaggggtggaacgcagtcaaagaatatgccgacccaatcgttgagatgttgcgggacagcggaaatgcagatgacaacatcatcattgtgggcagcccgaactggagtcagcgcccagatttagctgctgataatccaatcgatgatcaccacaccatgtataccgtgcacttctataccggctcgcatgcggcttcaacagaaagctacccaccagagacccccaactctgagcgcggcaacgtgatgagtaacactcgttacgcgctggagaacggcgttgccgtatttgctaccgaatggggcaccagtcaggcgaacggtgacggcggcccttattttgatgaggcagacgtgtggatcgagttccttaatgaaaataacatttcatgggcgaattggtccctgaccaacaagaacgaagtgagtggcgcgtttactccattcgagctgggcaagtcaaatgcaacctctctggatccgggtccggatcaggtgtgggtaccggaagagttgagcctgtctggagaatacgtccgcgctcgtattaagggggttaattatgaaccgattgatcgcactaaatacacgaaagttctcggaggtggcgggtctaaaccgacggagaacaatgaagactttaatatcgtggcggtggcatcgaatttcgcgactaccgacctggacgcagaccggggtaaacttccgggcaaaaaacttcctttggaagtactgaaagaaatggaagcgaacgcacgtaaagccgggtgtacccgtggttgcttaatctgcctcagtcacattaagtgcacaccgaagatgaaaaagttcattcctggccggtgccatacctatgaaggtgataaagaaagtgcccagggcggcattggtgaagccattgtagacattccggaaatcccaggtttcaaagacttagaaccaatggaacagttcatcgcgcaagtggacctgtgcgtggattgcacgacgggttgtcttaaagggctggccaatgtccagtgctcagatctgctgaagaaatggctgcctcaacgctgcgctacgtttgcgagcaagattcagggccaggtggacaagattaagggtgcaggcggtgaccatcatcaccaccatcactaataatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z