LOCUS       BBa_K1732004            1765 bp    DNA     linear       25-Nov-2024
DEFINITION  pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015
ACCESSION   BBa_K1732004
VERSION     BBa_K1732004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..1098
                     /label=CDcel Domain
     misc_feature    64..1098
                     /label=BBa_K1732002
     misc_feature    1107..1609
                     /label=GLucCO
     misc_feature    1610..1628
                     /label=6X-His
     CDS             1107..1628
                     /label=BBa_K1732027
     stem_loop       1648..1691
                     /label=stem_loop
     misc_feature    1637..1716
                     /label=BBa_B0010
     terminator      1637..1716
                     /label=BBa_B0010
     stem_loop       1732..1751
                     /label=T7 TE
     misc_feature    1758..1758
                     /label=stop
     polyA_site      1752..1765
                     /label=polya
     misc_feature    1725..1765
                     /label=BBa_B0012
     terminator      1725..1765
                     /label=BBa_B0012
     terminator      1637..1765
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gaggaagtgg atggccagat gaccctggtc gatcaacatg gcgaaaagat ccaactccgc
      121 ggtatgagca cccacgggtt acagtggttc ccggaaattc tgaacgataa tgcgtacaaa
      181 gctctggcta acgactggga atctaatatg attcgccttg cgatgtatgt cggagaaaac
      241 gggtatgcgt caaatcctga attgattaag tctcgcgtta ttaaaggtat tgatttagcg
      301 attgaaaatg atatgtatgt tatcgtggat tggcatgtcc atgctccggg cgacccgcgc
      361 gatccagtat atgctggtgc ggaagatttt ttccgcgata tcgcagcgtt atatccgaat
      421 aacccgcata tcatttacga actggctaat gagccgtctt ccaataacaa tggcggcgca
      481 ggcattccga acaacgagga ggggtggaac gcagtcaaag aatatgccga cccaatcgtt
      541 gagatgttgc gggacagcgg aaatgcagat gacaacatca tcattgtggg cagcccgaac
      601 tggagtcagc gcccagattt agctgctgat aatccaatcg atgatcacca caccatgtat
      661 accgtgcact tctataccgg ctcgcatgcg gcttcaacag aaagctaccc accagagacc
      721 cccaactctg agcgcggcaa cgtgatgagt aacactcgtt acgcgctgga gaacggcgtt
      781 gccgtatttg ctaccgaatg gggcaccagt caggcgaacg gtgacggcgg cccttatttt
      841 gatgaggcag acgtgtggat cgagttcctt aatgaaaata acatttcatg ggcgaattgg
      901 tccctgacca acaagaacga agtgagtggc gcgtttactc cattcgagct gggcaagtca
      961 aatgcaacct ctctggatcc gggtccggat caggtgtggg taccggaaga gttgagcctg
     1021 tctggagaat acgtccgcgc tcgtattaag ggggttaatt atgaaccgat tgatcgcact
     1081 aaatacacga aagttctcta ctagagaaac cgacggagaa caatgaagac tttaatatcg
     1141 tggcggtggc atcgaatttc gcgactaccg acctggacgc agaccggggt aaacttccgg
     1201 gcaaaaaact tcctttggaa gtactgaaag aaatggaagc gaacgcacgt aaagccgggt
     1261 gtacccgtgg ttgcttaatc tgcctcagtc acattaagtg cacaccgaag atgaaaaagt
     1321 tcattcctgg ccggtgccat acctatgaag gtgataaaga aagtgcccag ggcggcattg
     1381 gtgaagccat tgtagacatt ccggaaatcc caggtttcaa agacttagaa ccaatggaac
     1441 agttcatcgc gcaagtggac ctgtgcgtgg attgcacgac gggttgtctt aaagggctgg
     1501 ccaatgtcca gtgctcagat ctgctgaaga aatggctgcc tcaacgctgc gctacgtttg
     1561 cgagcaagat tcagggccag gtggacaaga ttaagggtgc aggcggtgac catcatcacc
     1621 accatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1681 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1741 tcgggtgggc ctttctgcgt ttata
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