BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1732002 1 BBa_K1732002 CDcel 2015-09-06T11:00:00Z 2015-09-25T12:40:34Z This sequence was engineered by Cheryl Telmer. Provided by the Bruchez Lab at Carnegie Mellon University. CDcel domain allows for the gene of interest to synthesize proteins that will be secreted into the media. false false _2154_ 27195 27195 9 false The backbone is compatible with the backbone pSB1C3 and is codon optimized for E.coli bacterial cells. false Donna Lee annotation2477346 1 CDcel Domain range2477346 1 1 1035 BBa_K1732004 1 BBa_K1732004 pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015 2015-09-06T11:00:00Z 2015-09-18T12:54:16Z Sequence was obtained from Cheryl Telmer from the Bruchez lab in Carnegie Mellon University. J23100 constitutive promoter (BBa_J23100), a RBS (BBa_B0034), CDcel domain (BBa_K1732002), Gaussia luciferase (BBa_K1732003), and B0015 terminator (BBa_B0015). This DNA sequence, expressed from a constitutive, strong promoter allows for the Gaussia luciferase protein to be synthesized and was targeted extrecellularly into the media. The Gaussia luciferase proteins were reacted with the optimal concentration of coelenterazine to express luminescence, which was used to test the Carnegie Mellon DIY luminometer. false false _2154_ 27195 27195 9 false E.coli was codon optimized for E.coli. false Donna Lee component2469667 1 BBa_K1732002 component2469670 1 BBa_K1732027 component2469664 1 BBa_J23100 component2469677 1 BBa_B0015 component2469666 1 BBa_B0034 annotation2469670 1 BBa_K1732027 range2469670 1 1107 1628 annotation2469677 1 BBa_B0015 range2469677 1 1637 1765 annotation2469667 1 BBa_K1732002 range2469667 1 64 1098 annotation2469664 1 BBa_J23100 range2469664 1 1 35 annotation2469666 1 BBa_B0034 range2469666 1 44 55 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1732027 1 BBa_K1732027 GLuc-His 2015-09-17T11:00:00Z 2016-01-19T01:23:44Z BBa_K1732003 Gaussia Luciferase (BBa_K1732003) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools. false false _2154_ 4206 27195 9 Not in stock false 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools. false Donna Lee annotation2469630 1 GLucCO range2469630 1 1 503 annotation2469631 1 6X-His range2469631 1 504 522 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1732027_sequence 1 aaaccgacggagaacaatgaagactttaatatcgtggcggtggcatcgaatttcgcgactaccgacctggacgcagaccggggtaaacttccgggcaaaaaacttcctttggaagtactgaaagaaatggaagcgaacgcacgtaaagccgggtgtacccgtggttgcttaatctgcctcagtcacattaagtgcacaccgaagatgaaaaagttcattcctggccggtgccatacctatgaaggtgataaagaaagtgcccagggcggcattggtgaagccattgtagacattccggaaatcccaggtttcaaagacttagaaccaatggaacagttcatcgcgcaagtggacctgtgcgtggattgcacgacgggttgtcttaaagggctggccaatgtccagtgctcagatctgctgaagaaatggctgcctcaacgctgcgctacgtttgcgagcaagattcagggccaggtggacaagattaagggtgcaggcggtgaccatcatcaccaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_K1732002_sequence 1 gaagtggatggccagatgaccctggtcgatcaacatggcgaaaagatccaactccgcggtatgagcacccacgggttacagtggttcccggaaattctgaacgataatgcgtacaaagctctggctaacgactgggaatctaatatgattcgccttgcgatgtatgtcggagaaaacgggtatgcgtcaaatcctgaattgattaagtctcgcgttattaaaggtattgatttagcgattgaaaatgatatgtatgttatcgtggattggcatgtccatgctccgggcgacccgcgcgatccagtatatgctggtgcggaagattttttccgcgatatcgcagcgttatatccgaataacccgcatatcatttacgaactggctaatgagccgtcttccaataacaatggcggcgcaggcattccgaacaacgaggaggggtggaacgcagtcaaagaatatgccgacccaatcgttgagatgttgcgggacagcggaaatgcagatgacaacatcatcattgtgggcagcccgaactggagtcagcgcccagatttagctgctgataatccaatcgatgatcaccacaccatgtataccgtgcacttctataccggctcgcatgcggcttcaacagaaagctacccaccagagacccccaactctgagcgcggcaacgtgatgagtaacactcgttacgcgctggagaacggcgttgccgtatttgctaccgaatggggcaccagtcaggcgaacggtgacggcggcccttattttgatgaggcagacgtgtggatcgagttccttaatgaaaataacatttcatgggcgaattggtccctgaccaacaagaacgaagtgagtggcgcgtttactccattcgagctgggcaagtcaaatgcaacctctctggatccgggtccggatcaggtgtgggtaccggaagagttgagcctgtctggagaatacgtccgcgctcgtattaagggggttaattatgaaccgattgatcgcactaaatacacgaaagttctc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1732004_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagaggaagtggatggccagatgaccctggtcgatcaacatggcgaaaagatccaactccgcggtatgagcacccacgggttacagtggttcccggaaattctgaacgataatgcgtacaaagctctggctaacgactgggaatctaatatgattcgccttgcgatgtatgtcggagaaaacgggtatgcgtcaaatcctgaattgattaagtctcgcgttattaaaggtattgatttagcgattgaaaatgatatgtatgttatcgtggattggcatgtccatgctccgggcgacccgcgcgatccagtatatgctggtgcggaagattttttccgcgatatcgcagcgttatatccgaataacccgcatatcatttacgaactggctaatgagccgtcttccaataacaatggcggcgcaggcattccgaacaacgaggaggggtggaacgcagtcaaagaatatgccgacccaatcgttgagatgttgcgggacagcggaaatgcagatgacaacatcatcattgtgggcagcccgaactggagtcagcgcccagatttagctgctgataatccaatcgatgatcaccacaccatgtataccgtgcacttctataccggctcgcatgcggcttcaacagaaagctacccaccagagacccccaactctgagcgcggcaacgtgatgagtaacactcgttacgcgctggagaacggcgttgccgtatttgctaccgaatggggcaccagtcaggcgaacggtgacggcggcccttattttgatgaggcagacgtgtggatcgagttccttaatgaaaataacatttcatgggcgaattggtccctgaccaacaagaacgaagtgagtggcgcgtttactccattcgagctgggcaagtcaaatgcaacctctctggatccgggtccggatcaggtgtgggtaccggaagagttgagcctgtctggagaatacgtccgcgctcgtattaagggggttaattatgaaccgattgatcgcactaaatacacgaaagttctctactagagaaaccgacggagaacaatgaagactttaatatcgtggcggtggcatcgaatttcgcgactaccgacctggacgcagaccggggtaaacttccgggcaaaaaacttcctttggaagtactgaaagaaatggaagcgaacgcacgtaaagccgggtgtacccgtggttgcttaatctgcctcagtcacattaagtgcacaccgaagatgaaaaagttcattcctggccggtgccatacctatgaaggtgataaagaaagtgcccagggcggcattggtgaagccattgtagacattccggaaatcccaggtttcaaagacttagaaccaatggaacagttcatcgcgcaagtggacctgtgcgtggattgcacgacgggttgtcttaaagggctggccaatgtccagtgctcagatctgctgaagaaatggctgcctcaacgctgcgctacgtttgcgagcaagattcagggccaggtggacaagattaagggtgcaggcggtgaccatcatcaccaccatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z