LOCUS       BBa_K1732005            1149 bp    DNA     linear       22-May-2024
DEFINITION  J23100-B0034-Renilla-B0015
ACCESSION   BBa_K1732005
VERSION     BBa_K1732005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..993
                     /label=RLuc
     misc_feature    994..1012
                     /label=6X-His
     CDS             62..1012
                     /label=BBa_K1732028
     stem_loop       1032..1075
                     /label=stem_loop
     misc_feature    1021..1100
                     /label=BBa_B0010
     terminator      1021..1100
                     /label=BBa_B0010
     stem_loop       1116..1135
                     /label=T7 TE
     misc_feature    1142..1142
                     /label=stop
     polyA_site      1136..1149
                     /label=polya
     misc_feature    1109..1149
                     /label=BBa_B0012
     terminator      1109..1149
                     /label=BBa_B0012
     terminator      1021..1149
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaccagt aaggtgtacg acccagaaca gcggaagcgc atgatcactg ggccgcagtg
      121 gtgggcccgt tgcaaacaaa tgaacgttct ggattcgttt attaactatt atgattctga
      181 aaagcatgct gagaacgcgg tgattttcct gcatggcaat gcggcctctt cgtatctgtg
      241 gcgccatgtt gttccacata tcgaaccggt agcccgctgc atcattccgg atttaatcgg
      301 tatgggcaaa agcggcaaat ccgggaacgg ctcttatcgt ctgcttgatc attataaata
      361 tctgactgcc tggttcgaac tgctgaatct tcctaagaaa atcatctttg ttggccatga
      421 ttggggagcg tgcctggcct tccactattc ttatgaacac caggataaaa tcaaagctat
      481 tgtgcatgcc gaaagcgttg tggacgttat cgaaagctgg gatgaatggc ccgatatcga
      541 agaagacatc gcgttgatta agtccgaaga aggggagaaa atggtcctgg aaaataattt
      601 ctttgtcgaa acgatgctgc catccaagat catgcggaag ctcgagccgg aggaatttgc
      661 ggcgtatctg gaaccgttca aagaaaaggg tgaagtgcgc cgcccgaccc tgtcgtggcc
      721 gcgtgaaatt ccattggtga aaggcggaaa accagacgta gtgcaaattg ttcgtaacta
      781 caatgcctat ctgcgtgcaa gcgatgacct gcccaaaatg ttcattgagt cggatccagg
      841 cttcttcagc aacgccatcg ttgagggcgc caagaaattt ccaaacacag aatttgtcaa
      901 ggtcaaggga ctgcattttt ctcaggaaga tgccccggac gagatgggga agtatatcaa
      961 aagttttgtg gaacgtgtgc tgaaaaacga gcagcatcat caccaccatc actactagag
     1021 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1081 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1141 gcgtttata
//