BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1732029 1 BBa_K1732029 FireflyCO-His 2015-09-17T11:00:00Z 2016-01-19T01:24:22Z BBa_I712019 Firefly luciferase (BBa_I712019) with 6X-His tagged for easier protein purification. Sequence was codon optimized by IDT codon optimization tools. false false _2154_ 4206 27195 9 false 6X-His tagged for easier protein purification. Sequence was codon optimized by IDT codon optimization tools. false Donna Lee annotation2469654 1 FireflyCO range2469654 1 1 1649 annotation2469655 1 6XHis range2469655 1 1650 1668 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1732006 1 BBa_K1732006 pSB1C3-J23100-B0034-Firefly-B0015 2015-09-08T11:00:00Z 2015-09-18T01:05:40Z Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University. J23100-B0034-Firefly-B0015 false false _2154_ 27195 27195 9 false Sequence is codon optimized for E.coli false Donna Lee component2469833 1 BBa_B0034 component2469836 1 BBa_K1732029 component2469843 1 BBa_B0015 component2469831 1 BBa_J23100 annotation2469843 1 BBa_B0015 range2469843 1 1738 1866 annotation2469836 1 BBa_K1732029 range2469836 1 62 1729 annotation2469831 1 BBa_J23100 range2469831 1 1 35 annotation2469833 1 BBa_B0034 range2469833 1 44 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1732029_sequence 1 atggaggatgcgaaaaacattaaaaaaggcccagcccctttctatccgctggaggacggtaccgccggtgagcagttgcataaagcaatgaagcgctatgcattagtccctggtacgattgcttttacggatgcgcacattgaagtcaatatcacgtacgccgaatatttcgagatgagcgtccgcctggcggaagcgatgaagcgttatggtttgaacaccaaccaccgcatcgtggtatgtagcgaaaactcattacagttctttatgcctgtgttgggggccttgtttattggtgttgcggttgcgccggccaatgatatttacaatgaacgtgaactgttaaattccatgaatatttcccagccaaccgttgtgttcgtgtctaaaaagggcttacagaagatcttaaatgttcagaaaaagctgcctatcatccaaaaaattatcattatggattccaaaacggactatcaaggttttcagagcatgtacacctttgttacgtcccatcttccgccgggtttcaacgaatatgacttcgtgcccgaatcatttgatcgtgacaagactattgcgcttatcatgaatagttctggcagtactggcttgccaaaaggtgtggcacttccgcatcgcacagcgtgtgtgcgtttttctcatgcccgtgaccccattttcgggaaccagattattccggacaccgcgatcctgagtgtggttccctttcaccacggctttgggatgtttacgaccctggggtatcttatttgtggctttcgggtcgttctgatgtaccgttttgaagaggaactgttcttacgttcgctgcaagactataaaatccagtcagcgctgctggtgccaacattattctctttttttgctaaatctactctgattgataaatacgacctgtccaacttacacgagattgcctcggggggggcgccgttaagcaaagaagtgggggaagctgtagccaaacgtttccacctccccggtatccgtcagggttacgggctgacagagactacctcggccattttaatcaccccggaaggcgacgataaaccaggcgccgttgggaaggttgtaccgttttttgaagcgaaggtggttgacctggatacaggaaaaaccttaggcgtcaaccaacgcggggaactttgcgtgcggggcccgatgattatgagcggttatgtaaataatccggaagctacgaacgccctcattgacaaagatgggtggctgcattcaggtgatattgcctactgggatgaggacgagcatttctttattgtggatcgcctgaaatcgcttattaaatacaagggataccaagttgcaccagccgaacttgaatccattctgttgcagcatccaaatatcttcgacgcgggcgtcgcgggactgcctgatgacgatgccggtgaactgccagcagcggttgtcgtgcttgaacatggtaaaacgatgaccgaaaaagaaattgtggattacgtggcgagtcaggtcaccacggcaaaaaaactgcgcgggggcgtggtgtttgtagatgaggtgcccaaaggtctgaccggtaaactcgacgctcgcaaaattcgtgaaatcctgatcaaagctaaaaaggggggcaaaagtaaactgcatcatcaccaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_K1732006_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggaggatgcgaaaaacattaaaaaaggcccagcccctttctatccgctggaggacggtaccgccggtgagcagttgcataaagcaatgaagcgctatgcattagtccctggtacgattgcttttacggatgcgcacattgaagtcaatatcacgtacgccgaatatttcgagatgagcgtccgcctggcggaagcgatgaagcgttatggtttgaacaccaaccaccgcatcgtggtatgtagcgaaaactcattacagttctttatgcctgtgttgggggccttgtttattggtgttgcggttgcgccggccaatgatatttacaatgaacgtgaactgttaaattccatgaatatttcccagccaaccgttgtgttcgtgtctaaaaagggcttacagaagatcttaaatgttcagaaaaagctgcctatcatccaaaaaattatcattatggattccaaaacggactatcaaggttttcagagcatgtacacctttgttacgtcccatcttccgccgggtttcaacgaatatgacttcgtgcccgaatcatttgatcgtgacaagactattgcgcttatcatgaatagttctggcagtactggcttgccaaaaggtgtggcacttccgcatcgcacagcgtgtgtgcgtttttctcatgcccgtgaccccattttcgggaaccagattattccggacaccgcgatcctgagtgtggttccctttcaccacggctttgggatgtttacgaccctggggtatcttatttgtggctttcgggtcgttctgatgtaccgttttgaagaggaactgttcttacgttcgctgcaagactataaaatccagtcagcgctgctggtgccaacattattctctttttttgctaaatctactctgattgataaatacgacctgtccaacttacacgagattgcctcggggggggcgccgttaagcaaagaagtgggggaagctgtagccaaacgtttccacctccccggtatccgtcagggttacgggctgacagagactacctcggccattttaatcaccccggaaggcgacgataaaccaggcgccgttgggaaggttgtaccgttttttgaagcgaaggtggttgacctggatacaggaaaaaccttaggcgtcaaccaacgcggggaactttgcgtgcggggcccgatgattatgagcggttatgtaaataatccggaagctacgaacgccctcattgacaaagatgggtggctgcattcaggtgatattgcctactgggatgaggacgagcatttctttattgtggatcgcctgaaatcgcttattaaatacaagggataccaagttgcaccagccgaacttgaatccattctgttgcagcatccaaatatcttcgacgcgggcgtcgcgggactgcctgatgacgatgccggtgaactgccagcagcggttgtcgtgcttgaacatggtaaaacgatgaccgaaaaagaaattgtggattacgtggcgagtcaggtcaccacggcaaaaaaactgcgcgggggcgtggtgtttgtagatgaggtgcccaaaggtctgaccggtaaactcgacgctcgcaaaattcgtgaaatcctgatcaaagctaaaaaggggggcaaaagtaaactgcatcatcaccaccatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z