LOCUS       BBa_K1732008             933 bp    DNA     linear       22-Nov-2024
DEFINITION  pSB1C3-J23100-B0034-EGFP-His-B0015
ACCESSION   BBa_K1732008
VERSION     BBa_K1732008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..777
                     /label=EGFPCO
     misc_feature    778..794
                     /label=6X-His
     CDS             62..796
                     /label=BBa_K1732022
     stem_loop       816..859
                     /label=stem_loop
     misc_feature    805..884
                     /label=BBa_B0010
     terminator      805..884
                     /label=BBa_B0010
     stem_loop       900..919
                     /label=T7 TE
     misc_feature    926..926
                     /label=stop
     polyA_site      920..933
                     /label=polya
     misc_feature    893..933
                     /label=BBa_B0012
     terminator      893..933
                     /label=BBa_B0012
     terminator      805..933
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggttagc aagggtgaag agctgttcac gggtgtggtg cccattctgg tcgagctcga
      121 cggcgatgta aacggccata agttcagcgt ctcaggggaa ggtgagggag acgccaccta
      181 tgggaaactg acgctgaaat ttatctgtac gaccggtaaa ttgcctgttc cgtggccaac
      241 cctggttacc accctgacct acggtgttca gtgttttagc cgttatcctg atcatatgaa
      301 acagcacgat tttttcaaga gcgctatgcc ggaaggctac gttcaagaac gtaccatttt
      361 ctttaaagat gacggaaatt acaaaactcg tgcagaagtt aaatttgaag gtgataccct
      421 ggtaaatcgt atcgagttaa aaggtattga tttcaaagaa gacggcaaca tcttaggaca
      481 caaattggaa tataactaca actcacacaa cgtctacatt atggcggaca agcagaagaa
      541 cggtatcaaa gtcaacttta aaattcgtca taatatcgaa gacgggagcg tgcagctggc
      601 ggaccactat cagcagaaca ccccgatcgg tgatgggccg gtgctgctcc cggataacca
      661 ttatctgtcg acccagtccg ccctgagtaa agatccaaat gaaaaacgcg accatatggt
      721 gttgttagaa tttgtaaccg cggcgggtat cacgttaggc atggatgaac tgtacaaaca
      781 tcatcaccac catcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      901 gctcaccttc gggtgggcct ttctgcgttt ata
//