BBa_K1732008 1 BBa_K1732008 pSB1C3-J23100-B0034-EGFP-His-B0015 2015-09-08T11:00:00Z 2015-09-17T11:28:35Z Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University. pSB1C3-J23100-B0034-EGFP-His-B0015 false false _2154_ 27195 27195 9 false Sequence is codon optimized for E.coli false Donna Lee component2469170 1 BBa_B0015 component2469158 1 BBa_J23100 component2469163 1 BBa_K1732022 component2469160 1 BBa_B0034 annotation2469158 1 BBa_J23100 range2469158 1 1 35 annotation2469160 1 BBa_B0034 range2469160 1 44 55 annotation2469170 1 BBa_B0015 range2469170 1 805 933 annotation2469163 1 BBa_K1732022 range2469163 1 62 796 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1732022 1 BBa_K1732022 EGFPCO-His 2015-09-17T11:00:00Z 2016-01-21T02:51:07Z BBa_1491002 EGFP (BBa_1491002) with 6X-His tag for easier protein purification. Original sequence is codon optimized by IDT Codon Optimized Tool for E.coli. false false _2154_ 4206 27195 9 false Codon optimized for E.coli and His tag allows for easier protein purification. false Donna Lee annotation2469121 1 6X-His range2469121 1 717 733 annotation2469080 1 EGFPCO range2469080 1 1 716 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1732022_sequence 1 atggttagcaagggtgaagagctgttcacgggtgtggtgcccattctggtcgagctcgacggcgatgtaaacggccataagttcagcgtctcaggggaaggtgagggagacgccacctatgggaaactgacgctgaaatttatctgtacgaccggtaaattgcctgttccgtggccaaccctggttaccaccctgacctacggtgttcagtgttttagccgttatcctgatcatatgaaacagcacgattttttcaagagcgctatgccggaaggctacgttcaagaacgtaccattttctttaaagatgacggaaattacaaaactcgtgcagaagttaaatttgaaggtgataccctggtaaatcgtatcgagttaaaaggtattgatttcaaagaagacggcaacatcttaggacacaaattggaatataactacaactcacacaacgtctacattatggcggacaagcagaagaacggtatcaaagtcaactttaaaattcgtcataatatcgaagacgggagcgtgcagctggcggaccactatcagcagaacaccccgatcggtgatgggccggtgctgctcccggataaccattatctgtcgacccagtccgccctgagtaaagatccaaatgaaaaacgcgaccatatggtgttgttagaatttgtaaccgcggcgggtatcacgttaggcatggatgaactgtacaaacatcatcaccaccatcac BBa_K1732008_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggttagcaagggtgaagagctgttcacgggtgtggtgcccattctggtcgagctcgacggcgatgtaaacggccataagttcagcgtctcaggggaaggtgagggagacgccacctatgggaaactgacgctgaaatttatctgtacgaccggtaaattgcctgttccgtggccaaccctggttaccaccctgacctacggtgttcagtgttttagccgttatcctgatcatatgaaacagcacgattttttcaagagcgctatgccggaaggctacgttcaagaacgtaccattttctttaaagatgacggaaattacaaaactcgtgcagaagttaaatttgaaggtgataccctggtaaatcgtatcgagttaaaaggtattgatttcaaagaagacggcaacatcttaggacacaaattggaatataactacaactcacacaacgtctacattatggcggacaagcagaagaacggtatcaaagtcaactttaaaattcgtcataatatcgaagacgggagcgtgcagctggcggaccactatcagcagaacaccccgatcggtgatgggccggtgctgctcccggataaccattatctgtcgacccagtccgccctgagtaaagatccaaatgaaaaacgcgaccatatggtgttgttagaatttgtaaccgcggcgggtatcacgttaggcatggatgaactgtacaaacatcatcaccaccatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z