LOCUS       BBa_K1732009             930 bp    DNA     linear       25-Nov-2024
DEFINITION  pSB1C3-J23100-B0034-GFP-His-B0015
ACCESSION   BBa_K1732009
VERSION     BBa_K1732009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..774
                     /label=GFPCO
     misc_feature    775..792
                     /label=6x-His
     CDS             62..793
                     /label=BBa_K1732020
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     terminator      802..930
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgcgcaaa ggcgaagaac tgtttaccgg ggttgtgccg atcctggtcg aacttgatgg
      121 cgacgtaaac ggccacaagt ttagcgtatc cggagaaggc gaaggtgatg caacctacgg
      181 aaaactgacc ttgaagttca tttgtacgac cggtaaactt ccggttccgt ggccgaccct
      241 ggtgacgacc tttggttacg gcgtacagtg ttttgctcgt tacccagatc atatgaagca
      301 gcatgatttc tttaaatcgg caatgccgga aggctacgtg caagaacgta ccattttctt
      361 taaagacgat ggcaattata aaacccgcgc ggaggtgaaa ttcgaaggag atactctggt
      421 gaatcgcatc gagctgaagg ggatcgactt caaagaggac ggcaatattc tggggcataa
      481 attagaatat aattacaaca gtcacaatgt gtacattatg gcggacaaac agaagaatgg
      541 catcaaggtt aactttaaga tccgccacaa cattgaagac ggttccgttc aactggccga
      601 tcattatcag cagaatacgc ccattggtga tggacctgtg ctcctcccgg ataatcatta
      661 tctgagcact caatccgctc tgagtaagga cccaaacgag aagcgtgatc atatggtatt
      721 gctggaattt gttaccgcag ccggcattac ccacggcatg gacgagttat acaagcatca
      781 tcaccaccat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata
//