LOCUS       BBa_K1732015            2301 bp    DNA     linear       26-Dec-2024
DEFINITION  J23115-B0034-T7RNAP-LBD-B0034-YFP
ACCESSION   BBa_K1732015
VERSION     BBa_K1732015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23115
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1534
                     /label=BBa_K1491022
     misc_feature    1547..1550
                     /label=conserved
     RBS             1543..1554
                     /label=BBa_B0034
     misc_feature    1561..2277
                     /label=BBa_K864100
     misc_feature    2278..2295
                     /label=6x His
     CDS             1561..2301
                     /label=BBa_K1491004
ORIGIN
        1 tttatagcta gctcagccct tggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc
      121 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga
      181 gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa
      241 agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa
      301 gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc
      361 gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa
      421 gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc
      481 aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa
      541 gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa
      601 agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc
      661 gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct
      721 cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga
      781 ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc
      841 gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg
      901 cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca
      961 cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat
     1021 taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt
     1081 aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact
     1141 cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc
     1201 tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt
     1261 catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat
     1321 ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac
     1381 caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct
     1441 gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa
     1501 gttcattgag gaaaaccacg agaacatcat ggcttactag agaaagagga gaaatactag
     1561 atggttagca agggcgaaga actttttaca ggcgtagtac cgatcttagt tgaattagac
     1621 ggcgacgtta acggtcataa gtttagcgtg agcggtgagg gtgaaggtga cgcaacttac
     1681 ggcaagctga ccctgaagct gatttgcacg acgggtaagc tgccggtccc gtggcctacc
     1741 ctggtcacga ccttgggtta tggcgttcag tgtttcgcgc gttatccgga ccacatgaaa
     1801 caacacgatt tctttaagag cgcgatgcca gaaggctatg tgcaggagcg tacgatcttt
     1861 ttcaaagacg acggtaacta caagacgcgt gccgaagtca aattcgaagg cgacaccctg
     1921 gtgaatcgca ttgagctgaa gggtattgat ttcaaagagg atggcaatat cctgggtcac
     1981 aagctggagt acaattacaa ttcccacaac gtttacatca ccgcagataa acagaaaaat
     2041 ggcatcaaag cgaatttcaa aatccgtcac aacattgagg acggtggtgt tcaactggcg
     2101 gatcattacc agcaaaacac cccgattggt gacggtccgg tcctgttgcc ggataaccat
     2161 tatctgtctt accaaagcaa actgagcaaa gatccgaacg agaagcgcga ccacatggtg
     2221 ctgctggagt ttgtgaccgc tgccggtatt accctgggta tggatgagct gtataaacat
     2281 catcaccacc atcactaata a
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