LOCUS       BBa_K1732016            4242 bp    DNA     linear       26-Nov-2024
DEFINITION  pSB1C3-J23115-B0034-T7RNAP-sites-B0034-YFP
ACCESSION   BBa_K1732016
VERSION     BBa_K1732016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23115
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    2711..2728
                     /label=6x His Tag
     CDS             62..2728
                     /label=BBa_K1491030
     misc_feature    2735..3456
                     /label=OFPCO
     misc_feature    3457..3475
                     /label=6X-His
     CDS             2735..3475
                     /label=BBa_K1732023
     misc_feature    3488..3491
                     /label=conserved
     RBS             3484..3495
                     /label=BBa_B0034
     misc_feature    3502..4218
                     /label=BBa_K864100
     misc_feature    4219..4236
                     /label=6x His
     CDS             3502..4242
                     /label=BBa_K1491004
ORIGIN
        1 tttatagcta gctcagccct tggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc
      121 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga
      181 gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa
      241 agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa
      301 gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc
      361 gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa
      421 gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc
      481 aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa
      541 gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa
      601 agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc
      661 gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct
      721 cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga
      781 ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc
      841 gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg
      901 cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca
      961 cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat
     1021 taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt
     1081 aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact
     1141 cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc
     1201 tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt
     1261 catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat
     1321 ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac
     1381 caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct
     1441 gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa
     1501 gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac tggagaacac
     1561 ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg agtacgctgg
     1621 ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg acgggtcttg
     1681 ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa
     1741 cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga aagtcaacga
     1801 gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg tgaccgatga
     1861 gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg ctggtcaatg
     1921 gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc tggcttacgg
     1981 gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc cagctattga
     2041 ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca tggctaagct
     2101 gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga actggcttaa
     2161 gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag agattcttcg
     2221 caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc aggaatacaa
     2281 gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct tacagcctac
     2341 cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg gtatcgctcc
     2401 taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt gggcacacga
     2461 gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca ttccggctga
     2521 cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg agtcttgtga
     2581 tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc aattggacaa
     2641 aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag agtcggactt
     2701 cgcgttcgcg catcatcacc accatcacta ctagatggtg agcaaagggg aagaaaacaa
     2761 tatggctatt atcaaagaat ttatgcgttt taaagtgcgt atggaaggga gtgttaatgg
     2821 tcatgaattt gagatcgaag gtgaaggaga aggccgtccg tatgaaggat ttcagactgc
     2881 gaaattaaaa gtcaccaaag gaggaccgtt gccgtttgct tgggatattt tgtccccgca
     2941 gtttacatat ggctcgaaag catatgttaa acatccggcg gatattcctg attattttaa
     3001 attaagtttc ccggaaggct ttaagtggga acgcgtgatg aattttgaag acggcggtgt
     3061 tgtgaccgtt acccaagata gcagcttaca ggacggtgaa tttatctata aggttaagct
     3121 gcgcggcacg aatttcccgt ctgatggccc ggtcatgcaa aaaaaaacca tgggatggga
     3181 agcaagcagc gagcgtatgt accctgaaga tggcgcgctg aagggtgaaa tcaaaatgcg
     3241 cttgaaattg aaagatggcg gccattatac cagtgaagtc aaaactacct acaaagctaa
     3301 aaaaccggtc caactgccgg gcgcatatat tgtaggtatc aaactggaca ttacgtccca
     3361 caatgaagat tatacaattg ttgaacagta tgagcgtgca gaaggccgtc attctacggg
     3421 tggaatggat gagttatata aaggttcggg taccgcgcat catcaccacc atcactacta
     3481 gagaaagagg agaaatacta gatggttagc aagggcgaag aactttttac aggcgtagta
     3541 ccgatcttag ttgaattaga cggcgacgtt aacggtcata agtttagcgt gagcggtgag
     3601 ggtgaaggtg acgcaactta cggcaagctg accctgaagc tgatttgcac gacgggtaag
     3661 ctgccggtcc cgtggcctac cctggtcacg accttgggtt atggcgttca gtgtttcgcg
     3721 cgttatccgg accacatgaa acaacacgat ttctttaaga gcgcgatgcc agaaggctat
     3781 gtgcaggagc gtacgatctt tttcaaagac gacggtaact acaagacgcg tgccgaagtc
     3841 aaattcgaag gcgacaccct ggtgaatcgc attgagctga agggtattga tttcaaagag
     3901 gatggcaata tcctgggtca caagctggag tacaattaca attcccacaa cgtttacatc
     3961 accgcagata aacagaaaaa tggcatcaaa gcgaatttca aaatccgtca caacattgag
     4021 gacggtggtg ttcaactggc ggatcattac cagcaaaaca ccccgattgg tgacggtccg
     4081 gtcctgttgc cggataacca ttatctgtct taccaaagca aactgagcaa agatccgaac
     4141 gagaagcgcg accacatggt gctgctggag tttgtgaccg ctgccggtat taccctgggt
     4201 atggatgagc tgtataaaca tcatcaccac catcactaat aa
//