BBa_K1732016 1 BBa_K1732016 pSB1C3-J23115-B0034-T7RNAP-sites-B0034-YFP 2015-09-08T11:00:00Z 2015-09-18T09:22:59Z Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University. pSB1C3-J23115-B0034-T7RNAP-sites-B0034-YFP: Estrogen Control with sites false false _2154_ 27195 27195 9 false Sequence is codon optimized for E.coli false Donna Lee component2472671 1 BBa_B0034 component2472673 1 BBa_K1491030 component2472669 1 BBa_J23115 component2472681 1 BBa_K1491004 component2472678 1 BBa_B0034 component2472676 1 BBa_K1732023 annotation2472676 1 BBa_K1732023 range2472676 1 2735 3475 annotation2472673 1 BBa_K1491030 range2472673 1 62 2728 annotation2472669 1 BBa_J23115 range2472669 1 1 35 annotation2472681 1 BBa_K1491004 range2472681 1 3502 4242 annotation2472678 1 BBa_B0034 range2472678 1 3484 3495 annotation2472671 1 BBa_B0034 range2472671 1 44 55 BBa_J23115 1 BBa_J23115 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1732023 1 BBa_K1732023 OFPCO-His 2015-09-17T11:00:00Z 2015-09-17T11:33:58Z BBa_K1732011 OFP (BBa_K1732011) with 6X-His for easier protein purification. Original equation was codon optimized for E.coli via IDT codon optimization tool. false false _2154_ 27195 27195 9 false His tag for easier protein purification and sequence was codon optimized for E. coli. false Donna Lee annotation2469302 1 6X-His range2469302 1 723 741 annotation2469301 1 OFPCO range2469301 1 1 722 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1491004 1 BBa_K1491004 SuperYFP2-His 2014-07-14T11:00:00Z 2016-01-21T02:28:40Z SuperYFP2 is BBa_K864100 SuperYFP2 (BBa_K864100) with 6x His tag for protein purification. false false _1871_ 4206 22521 9 Not in stock false YFP+His false Danielle Peters annotation2380094 1 BBa_K864100 range2380094 1 1 717 annotation2380095 1 6x His range2380095 1 718 735 BBa_K1491030 1 BBa_K1491030 His Tagged T7 RNA Polymerase 2014-09-18T11:00:00Z 2015-05-08T01:10:44Z The T7 RNA polymerase is BBa_K145001 T7 RNA polymerase (BBa_K145001) with 6x His Tag false false _1871_ 0 22521 9 Not in stock false His Tagged T7 RNAP false Danielle Peters annotation2384127 1 6x His Tag range2384127 1 2650 2667 BBa_J23115_sequence 1 tttatagctagctcagcccttggtacaatgctagc BBa_K1491030_sequence 1 atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgcatcatcaccaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_K1732016_sequence 1 tttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgcatcatcaccaccatcactactagatggtgagcaaaggggaagaaaacaatatggctattatcaaagaatttatgcgttttaaagtgcgtatggaagggagtgttaatggtcatgaatttgagatcgaaggtgaaggagaaggccgtccgtatgaaggatttcagactgcgaaattaaaagtcaccaaaggaggaccgttgccgtttgcttgggatattttgtccccgcagtttacatatggctcgaaagcatatgttaaacatccggcggatattcctgattattttaaattaagtttcccggaaggctttaagtgggaacgcgtgatgaattttgaagacggcggtgttgtgaccgttacccaagatagcagcttacaggacggtgaatttatctataaggttaagctgcgcggcacgaatttcccgtctgatggcccggtcatgcaaaaaaaaaccatgggatgggaagcaagcagcgagcgtatgtaccctgaagatggcgcgctgaagggtgaaatcaaaatgcgcttgaaattgaaagatggcggccattataccagtgaagtcaaaactacctacaaagctaaaaaaccggtccaactgccgggcgcatatattgtaggtatcaaactggacattacgtcccacaatgaagattatacaattgttgaacagtatgagcgtgcagaaggccgtcattctacgggtggaatggatgagttatataaaggttcgggtaccgcgcatcatcaccaccatcactactagagaaagaggagaaatactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaacatcatcaccaccatcactaataa BBa_K1491004_sequence 1 atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaacatcatcaccaccatcactaataa BBa_K1732023_sequence 1 atggtgagcaaaggggaagaaaacaatatggctattatcaaagaatttatgcgttttaaagtgcgtatggaagggagtgttaatggtcatgaatttgagatcgaaggtgaaggagaaggccgtccgtatgaaggatttcagactgcgaaattaaaagtcaccaaaggaggaccgttgccgtttgcttgggatattttgtccccgcagtttacatatggctcgaaagcatatgttaaacatccggcggatattcctgattattttaaattaagtttcccggaaggctttaagtgggaacgcgtgatgaattttgaagacggcggtgttgtgaccgttacccaagatagcagcttacaggacggtgaatttatctataaggttaagctgcgcggcacgaatttcccgtctgatggcccggtcatgcaaaaaaaaaccatgggatgggaagcaagcagcgagcgtatgtaccctgaagatggcgcgctgaagggtgaaatcaaaatgcgcttgaaattgaaagatggcggccattataccagtgaagtcaaaactacctacaaagctaaaaaaccggtccaactgccgggcgcatatattgtaggtatcaaactggacattacgtcccacaatgaagattatacaattgttgaacagtatgagcgtgcagaaggccgtcattctacgggtggaatggatgagttatataaaggttcgggtaccgcgcatcatcaccaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z