BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K1732018 1 BBa_K1732018 pSB3K3-T7prom-B0034-Gaussia-T7term 2015-09-08T11:00:00Z 2015-09-18T04:08:20Z Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University. pSB3K3-T7prom-B0034-Gaussia-T7term false false _2154_ 27195 27195 9 false Sequence is codon optimized for E.coli false Donna Lee component2470870 1 BBa_I719005 component2470872 1 BBa_B0034 component2470875 1 BBa_K1732027 component2470876 1 BBa_B0016 annotation2470872 1 BBa_B0034 range2470872 1 32 43 annotation2470870 1 BBa_I719005 range2470870 1 1 23 annotation2470876 1 BBa_B0016 range2470876 1 582 629 annotation2470875 1 BBa_K1732027 range2470875 1 52 573 BBa_K1732027 1 BBa_K1732027 GLuc-His 2015-09-17T11:00:00Z 2016-01-19T01:23:44Z BBa_K1732003 Gaussia Luciferase (BBa_K1732003) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools. false false _2154_ 4206 27195 9 Not in stock false 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools. false Donna Lee annotation2469631 1 6X-His range2469631 1 504 522 annotation2469630 1 GLucCO range2469630 1 1 503 BBa_B0016 1 BBa_B0016 Terminator (T7 RNAP specific, T_Phi) 2003-12-04T12:00:00Z 2015-08-31T04:07:20Z Sri Kosuri Released HQ 2013 T7 gene 1 specific transcriptional terminator false true _1_ 0 24 7 In stock false false Sri Kosuri BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K1732027_sequence 1 aaaccgacggagaacaatgaagactttaatatcgtggcggtggcatcgaatttcgcgactaccgacctggacgcagaccggggtaaacttccgggcaaaaaacttcctttggaagtactgaaagaaatggaagcgaacgcacgtaaagccgggtgtacccgtggttgcttaatctgcctcagtcacattaagtgcacaccgaagatgaaaaagttcattcctggccggtgccatacctatgaaggtgataaagaaagtgcccagggcggcattggtgaagccattgtagacattccggaaatcccaggtttcaaagacttagaaccaatggaacagttcatcgcgcaagtggacctgtgcgtggattgcacgacgggttgtcttaaagggctggccaatgtccagtgctcagatctgctgaagaaatggctgcctcaacgctgcgctacgtttgcgagcaagattcagggccaggtggacaagattaagggtgcaggcggtgaccatcatcaccaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_K1732018_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagagaaaccgacggagaacaatgaagactttaatatcgtggcggtggcatcgaatttcgcgactaccgacctggacgcagaccggggtaaacttccgggcaaaaaacttcctttggaagtactgaaagaaatggaagcgaacgcacgtaaagccgggtgtacccgtggttgcttaatctgcctcagtcacattaagtgcacaccgaagatgaaaaagttcattcctggccggtgccatacctatgaaggtgataaagaaagtgcccagggcggcattggtgaagccattgtagacattccggaaatcccaggtttcaaagacttagaaccaatggaacagttcatcgcgcaagtggacctgtgcgtggattgcacgacgggttgtcttaaagggctggccaatgtccagtgctcagatctgctgaagaaatggctgcctcaacgctgcgctacgtttgcgagcaagattcagggccaggtggacaagattaagggtgcaggcggtgaccatcatcaccaccatcactactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_B0016_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z