LOCUS BBa_K1734007 1463 bp DNA linear 26-Nov-2024 DEFINITION C1 (p10 + HBM signal sequence + Nanoluc + T2A sequence + mRFP1 + 6xHis) ACCESSION BBa_K1734007 VERSION BBa_K1734007.1 FEATURES Location/Qualifiers promoter 7..116 /label=p10 promoter misc_feature 120..182 /label=Honeybee Melittin Signal Peptide CDS 183..707 /label=Nanoluc misc_feature 708..761 /label=T2A sequence CDS 762..1436 /label=mRFP1 misc_feature 1437..1454 /label=6XHis ORIGIN 1 tccggagacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca 61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaca 121 tgaagttcct ggttaacgtg gctctcgtct tcatggtggt ctacatctcc tacatttacg 181 ccatggctgg tgtcttcacg ctggaggact tcgttggaga ttggcgccag acagccggtt 241 acaacttgga ccaggttctg gaacaaggtg gcgtgtccag cctcttccag aacttgggcg 301 tttctgtgac tccaatccaa aggattgtgc tctcaggaga aaacggtttg aagatcgaca 361 ttcacgtcat cattccgtac gagggcctga gtggagatca gatgggacaa atcgaaaaga 421 ttttcaaagt ggtctacccc gtggacgatc accatttcaa agtcatcctg cattacggta 481 ctctcgtcat tgacggcgtt acacctaaca tgatcgatta cttcggacgc ccctacgagg 541 gtattgcagt tttcgacggc aagaaaatca ccgtcactgg cacactgtgg aacggaaaca 601 agatcattga cgaaaggctc atcaaccctg atggctccct gctcttcaga gtgacgatca 661 acggtgtcac cggatggaga ttgtgcgagc gtatcctggc taccggagag ggtagaggat 721 ccctgctcac ctgcggagac gtggaggaaa accctggtcc catggcatcc agcgaggacg 781 tgatcaagga gttcatgcgc ttcaaggttc gtatggaggg tagtgtgaac ggccacgagt 841 tcgaaattga gggtgaaggc gagggaaggc cctacgaagg cacacagacg gctaagctca 901 aagtcacgaa gggtggacct ctcccattcg catgggacat cttgagtcca cagttccaat 961 acggatcgaa ggcttacgtt aaacacccag ccgacatccc tgactacctg aagctcagct 1021 tccctgaggg tttcaagtgg gaacgcgtga tgaacttcga ggatggtggt gtggtcaccg 1081 tcactcagga ctcttcattg caagatggcg agttcatcta caaggttaaa ctgagaggta 1141 ccaacttccc ttccgacggc cccgtgatgc aaaagaaaac aatgggatgg gaggcaagca 1201 cggaacgcat gtacccagag gatggagcgc tgaagggtga aattaaaatg cgtttgaagc 1261 tgaaagacgg cggacactac gatgcagagg tcaagaccac ttacatggcg aagaaaccag 1321 ttcagctgcc gggtgcttac aagaccgaca tcaaactcga tattacttct cacaacgaag 1381 actacacaat cgtcgaacaa tacgagaggg ctgaaggcag acattcaact ggagcccacc 1441 atcaccatca ccattaacaa ttg //