LOCUS       BBa_K1734009            1901 bp    DNA     linear       23-Dec-2024
DEFINITION  C3 (OpIE2+ HBM signal sequence + Nanoluc + T2A sequence + mRFP1 + 
            6xHis)
ACCESSION   BBa_K1734009
VERSION     BBa_K1734009.1  
FEATURES             Location/Qualifiers
     promoter        7..554
                     /label=OPIE2 promoter
     misc_feature    558..620
                     /label=Honeybee Melittin Signal Peptide
     CDS             621..1145
                     /label=Nanoluc
     misc_feature    1146..1199
                     /label=T2A sequence
     CDS             1200..1874
                     /label=mRFP1
     misc_feature    1875..1892
                     /label=6XHis
ORIGIN
        1 tccggacatg atgataaaca atgtatggtg ctaatgttgc ttcaacaaca attctgttga
       61 actgtgtttt catgtttgcc aacaagcacc tttatactcg gtggcctccc caccaccaac
      121 ttttttgcac tgcaaaaaaa cacgcttttg cacgcgggcc catacatagt acaaactcta
      181 cgtttcgtag actattttac ataaatagtc tacaccgttg tatacgctcc aaatacacta
      241 ccacacattg aacctttttg cagtgcaaaa aagtacgtgt cggcagtcac gtaggccggc
      301 cttatcgggt cgcgtcctgt cacgtacgaa tcacattatc ggaccggacg agtgttgtct
      361 tatcgtgaca ggacgccagc ttcctgtgtt gctaaccgca gccggacgca actccttatc
      421 ggaacaggac gcgcctccat atcagccgcg cgttatctca tgcgcgtgac cggacacgag
      481 gcgcccgtcc cgcttatcgc gcctataaat acagcccgca acgatctggt aaacacagtt
      541 gaacagcatc tgttaacatg aagttcctgg ttaacgtggc tctcgtcttc atggtggtct
      601 acatctccta catttacgcc atggctggtg tcttcacgct ggaggacttc gttggagatt
      661 ggcgccagac agccggttac aacttggacc aggttctgga acaaggtggc gtgtccagcc
      721 tcttccagaa cttgggcgtt tctgtgactc caatccaaag gattgtgctc tcaggagaaa
      781 acggtttgaa gatcgacatt cacgtcatca ttccgtacga gggcctgagt ggagatcaga
      841 tgggacaaat cgaaaagatt ttcaaagtgg tctaccccgt ggacgatcac catttcaaag
      901 tcatcctgca ttacggtact ctcgtcattg acggcgttac acctaacatg atcgattact
      961 tcggacgccc ctacgagggt attgcagttt tcgacggcaa gaaaatcacc gtcactggca
     1021 cactgtggaa cggaaacaag atcattgacg aaaggctcat caaccctgat ggctccctgc
     1081 tcttcagagt gacgatcaac ggtgtcaccg gatggagatt gtgcgagcgt atcctggcta
     1141 ccggagaggg tagaggatcc ctgctcacct gcggagacgt ggaggaaaac cctggtccca
     1201 tggcatccag cgaggacgtg atcaaggagt tcatgcgctt caaggttcgt atggagggta
     1261 gtgtgaacgg ccacgagttc gaaattgagg gtgaaggcga gggaaggccc tacgaaggca
     1321 cacagacggc taagctcaaa gtcacgaagg gtggacctct cccattcgca tgggacatct
     1381 tgagtccaca gttccaatac ggatcgaagg cttacgttaa acacccagcc gacatccctg
     1441 actacctgaa gctcagcttc cctgagggtt tcaagtggga acgcgtgatg aacttcgagg
     1501 atggtggtgt ggtcaccgtc actcaggact cttcattgca agatggcgag ttcatctaca
     1561 aggttaaact gagaggtacc aacttccctt ccgacggccc cgtgatgcaa aagaaaacaa
     1621 tgggatggga ggcaagcacg gaacgcatgt acccagagga tggagcgctg aagggtgaaa
     1681 ttaaaatgcg tttgaagctg aaagacggcg gacactacga tgcagaggtc aagaccactt
     1741 acatggcgaa gaaaccagtt cagctgccgg gtgcttacaa gaccgacatc aaactcgata
     1801 ttacttctca caacgaagac tacacaatcg tcgaacaata cgagagggct gaaggcagac
     1861 attcaactgg agcccaccat caccatcacc attaacaatt g
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