LOCUS       BBa_K1734010            1436 bp    DNA     linear       20-May-2024
DEFINITION  C4 (pPH +CL signal peptide + Nanoluc + T2A sequence + mRFP1 + 
            6xHis)
ACCESSION   BBa_K1734010
VERSION     BBa_K1734010.1  
FEATURES             Location/Qualifiers
     promoter        7..98
                     /label=polyhedrin promoter
     CDS             99..582
                     /label=Nanoluc
     misc_feature    681..734
                     /label=T2A
     CDS             735..1409
                     /label=mRFP1
     misc_feature    1410..1427
                     /label=6XHis
ORIGIN
        1 tccggaatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
       61 gttttcgtaa cagttttgta ataaaaaaac ctataaataa catgcgctcc ctgctcatct
      121 tggtgctgtg cttcctccct ttggctgccc tgggtatggc tggtgtcttc acgctggagg
      181 acttcgttgg agattggcgc cagacagccg gttacaactt ggaccaggtt ctggaacaag
      241 gtggcgtgtc cagcctcttc cagaacttgg gcgtttctgt gactccaatc caaaggattg
      301 tgctctcagg agaaaacggt ttgaagatcg acattcacgt catcattccg tacgagggcc
      361 tgagtggaga tcagatggga caaatcgaaa agattttcaa agtggtctac cccgtggacg
      421 atcaccattt caaagtcatc ctgcattacg gtactctcgt cattgacggc gttacaccta
      481 acatgatcga ttacttcgga cgcccctacg agggtattgc agttttcgac ggcaagaaaa
      541 tcaccgtcac tggcacactg tggaacggaa acaagatcat tgacgaaagg ctcatcaacc
      601 ctgatggctc cctgctcttc agagtgacga tcaacggtgt caccggatgg agattgtgcg
      661 agcgtatcct ggctaccgga gagggtagag gatccctgct cacctgcgga gacgtggagg
      721 aaaaccctgg tcccatggca tccagcgagg acgtgatcaa ggagttcatg cgcttcaagg
      781 ttcgtatgga gggtagtgtg aacggccacg agttcgaaat tgagggtgaa ggcgagggaa
      841 ggccctacga aggcacacag acggctaagc tcaaagtcac gaagggtgga cctctcccat
      901 tcgcatggga catcttgagt ccacagttcc aatacggatc gaaggcttac gttaaacacc
      961 cagccgacat ccctgactac ctgaagctca gcttccctga gggtttcaag tgggaacgcg
     1021 tgatgaactt cgaggatggt ggtgtggtca ccgtcactca ggactcttca ttgcaagatg
     1081 gcgagttcat ctacaaggtt aaactgagag gtaccaactt cccttccgac ggccccgtga
     1141 tgcaaaagaa aacaatggga tgggaggcaa gcacggaacg catgtaccca gaggatggag
     1201 cgctgaaggg tgaaattaaa atgcgtttga agctgaaaga cggcggacac tacgatgcag
     1261 aggtcaagac cacttacatg gcgaagaaac cagttcagct gccgggtgct tacaagaccg
     1321 acatcaaact cgatattact tctcacaacg aagactacac aatcgtcgaa caatacgaga
     1381 gggctgaagg cagacattca actggagccc accatcacca tcaccattaa caattg
//