LOCUS       BBa_K1734011            2633 bp    DNA     linear       22-Nov-2024
DEFINITION  C5 (pPH + HBM signal sequence + Nanoluc + T2A sequence + EGFR + 
            6xHis)
ACCESSION   BBa_K1734011
VERSION     BBa_K1734011.1  
FEATURES             Location/Qualifiers
     promoter        7..98
                     /label=polyhendrin promoter
     misc_feature    102..164
                     /label=Honeybee Melittin Signal Peptide
     CDS             165..689
                     /label=Nanoluc
     misc_feature    690..743
                     /label=T2A
     CDS             744..2606
                     /label=Epidermal Growth Factor Receptor
     misc_feature    2607..2624
                     /label=6XHis
ORIGIN
        1 tccggaatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
       61 gttttcgtaa cagttttgta ataaaaaaac ctataaataa catgaagttc ctggttaacg
      121 tggctctcgt cttcatggtg gtctacatct cctacattta cgccatggct ggtgtcttca
      181 cgctggagga cttcgttgga gattggcgcc agacagccgg ttacaacttg gaccaggttc
      241 tggaacaagg tggcgtgtcc agcctcttcc agaacttggg cgtttctgtg actccaatcc
      301 aaaggattgt gctctcagga gaaaacggtt tgaagatcga cattcacgtc atcattccgt
      361 acgagggcct gagtggagat cagatgggac aaatcgaaaa gattttcaaa gtggtctacc
      421 ccgtggacga tcaccatttc aaagtcatcc tgcattacgg tactctcgtc attgacggcg
      481 ttacacctaa catgatcgat tacttcggac gcccctacga gggtattgca gttttcgacg
      541 gcaagaaaat caccgtcact ggcacactgt ggaacggaaa caagatcatt gacgaaaggc
      601 tcatcaaccc tgatggctcc ctgctcttca gagtgacgat caacggtgtc accggatgga
      661 gattgtgcga gcgtatcctg gctaccggag agggtagagg atccctgctc acctgcggag
      721 acgtggagga aaaccctggt cccctggagg aaaagaaagt gtgccagggc acttcaaaca
      781 agctcacaca gctgggaacg ttcgaagacc acttcctcag tttgcagagg atgttcaaca
      841 actgcgaagt ggtcctgggc aacctcgaga tcacatacgt ccagagaaac tacgatttgt
      901 ctttcctgaa gacgatccaa gaagtggctg gttacgtcct catcgccttg aacactgtgg
      961 aaaggattcc tctggagaac ctccaaatca ttagaggcaa catgtactac gagaactcat
     1021 acgcattggc ggtcctgagt aactacgacg ctaacaagac aggcctcaaa gaattgccaa
     1081 tgaggaacct ccaggagatc ttgcatggag ctgtgagatt cagcaacaac ccggccctgt
     1141 gcaacgtgga gtctatccaa tggcgtgaca ttgtctccag cgatttcctc tctaacatgt
     1201 caatggactt ccagaaccac ttgggctcat gccaaaagtg tgatccaagt tgtccgaacg
     1261 gatcgtgctg gggtgctggc gaggaaaact gccagaagct gacaaaaatc atttgcgccc
     1321 agcaatgtag cggacgctgc cgtggcaagt ctccatcaga ctgctgtcac aaccaatgtg
     1381 ctgccggttg caccggcccg agggaatctg attgcctcgt gtgtaggaag ttcagagacg
     1441 aggctacctg taaagatact tgccctcccc tgatgctcta caaccctacc acttaccaga
     1501 tggacgtcaa ccccgaaggc aagtactcct tcggagccac ctgtgttaag aaatgcccac
     1561 gcaactacgt tgtgactgat cacggttcgt gtgtccgtgc atgcggcgcg gactcctacg
     1621 aaatggagga agatggtgtg cgcaagtgca agaaatgtga gggcccttgt cgtaaagtct
     1681 gcaacggaat cggtattggc gagttcaagg acagtctctc gatcaacgca accaacatta
     1741 agcatttcaa aaactgcact tccatcagcg gagacctgca cattctccct gtcgccttca
     1801 ggggtgacag cttcacacac acgccaccgt tggaccccca ggaattggat atcctgaaga
     1861 ccgttaaaga gatcactgga ttcctgctca ttcaagcatg gcccgagaac agaaccgact
     1921 tgcatgcgtt cgagaacctg gaaatcatta ggggaagaac taagcagcac ggtcaattct
     1981 ccctggctgt cgttagcctc aacatcacat ctttgggtct gcgctcactg aaggaaatta
     2041 gtgacggcga tgtgatcatt tcgggaaaca aaaacctgtg ctacgccaac acgatcaact
     2101 ggaagaaact cttcggaaca tccggtcaga agacgaaaat cattagcaac cgcggagaga
     2161 actcttgcaa ggcaactggt caagtctgtc atgcgctgtg ctcaccagaa ggatgctggg
     2221 gacctgagcc aagagactgc gtttcgtgtc gtaacgtgtc ccgcggtcgt gaatgtgttg
     2281 ataagtgcaa cttgctggag ggcgaacctc gcgagttcgt ggaaaacagt gagtgcatcc
     2341 agtgtcaccc agagtgtctg ccgcaagcca tgaacatcac ctgcactgga cgtggtcccg
     2401 acaactgcat ccagtgtgca cattacattg atggtccaca ctgtgtcaag acatgcccgg
     2461 cgggtgttat gggcgaaaac aacacgttgg tttggaaata cgctgacgcc ggacacgtgt
     2521 gccatctgtg tcaccccaac tgtacatacg gttgcacggg acctggactg gagggttgcc
     2581 caaccaacgg ccctaagatc ccctcccacc atcaccatca ccattaacaa ttg
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