LOCUS       BBa_K1740004            1642 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac+RBS+145N+MCS+145N+stop codon
ACCESSION   BBa_K1740004
VERSION     BBa_K1740004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=pLac
     RBS             209..222
                     /label=RBS
     CDS             227..766
                     /label=Dronpa 145N
     misc_feature    465..467
                     /label=145Lys to 145Asn
     misc_feature    911..916
                     /label=Xho I
     misc_feature    917..922
                     /label=Hind III
     misc_feature    930..935
                     /label=Nde I
     misc_feature    939..944
                     /label=Kpn I
     misc_feature    941..946
                     /label=Sal I
     misc_feature    951..956
                     /label=Sac I
     misc_feature    959..964
                     /label=BamH I
     CDS             965..1636
                     /label=Dronpa 145N
     misc_feature    1203..1205
                     /label=145Lys to 145Asn
     misc_feature    1637..1642
                     /label=Double stop codon
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cagtcatcaa
      241 gcctgacatg aaaattaaat tgcgaatgga aggtgctgta aacgggcacc cgttcgctat
      301 agaaggggta gggttgggca agcccttcga aggaaagcag agtatggatc ttaaagttaa
      361 agagggtgga ccgttaccat ttgcctatga cattctcact acagtattct gctatggcaa
      421 ccgtgttttc gcaaagtatc cagaaaatat cgttgactac ttcaaacagt cgtttcccga
      481 gggctattcg tgggagcgct ctatgaatta cgaggacggt ggtatctgta atgccaccaa
      541 cgatattacc ctggacggtg actgttatat ttatgaaatc cgctttgatg gtgtgaattt
      601 tcctgcaaat ggtccggtga tgcaaaaacg gaccgttaaa tgggagccga gcacggaaaa
      661 cctatatgtc cgcgatggcg tgctgaaagg cgatgtgaac atggcgctga gcttagaagg
      721 cggcggccat taccgttgcg attttaaaac gacctacaaa gcgaaaaaag tcgtccaact
      781 gccggattac cactttgtgg atcatcatat cgaaattaaa tcccatgata aagattacag
      841 caacgtgaac ctgcacgaac atgccgaagc gcacagtgaa ctgccgcgtc aggcgaaact
      901 cgagtaataa ctcgagaagc tttggccaca tatgggtacc gtcgacgagc tccccggggg
      961 atccatgtca gtcatcaagc ctgacatgaa aattaaattg cgaatggaag gtgctgtaaa
     1021 cgggcacccg ttcgctatag aaggggtagg gttgggcaag cccttcgaag gaaagcagag
     1081 tatggatctt aaagttaaag agggtggacc gttaccattt gcctatgaca ttctcactac
     1141 agtattctgc tatggcaacc gtgttttcgc aaagtatcca gaaaatatcg ttgactactt
     1201 caaacagtcg tttcccgagg gctattcgtg ggagcgctct atgaattacg aggacggtgg
     1261 tatctgtaat gccaccaacg atattaccct ggacggtgac tgttatattt atgaaatccg
     1321 ctttgatggt gtgaattttc ctgcaaatgg tccggtgatg caaaaacgga ccgttaaatg
     1381 ggagccgagc acggaaaacc tatatgtccg cgatggcgtg ctgaaaggcg atgtgaacat
     1441 ggcgctgagc ttagaaggcg gcggccatta ccgttgcgat tttaaaacga cctacaaagc
     1501 gaaaaaagtc gtccaactgc cggattacca ctttgtggat catcatatcg aaattaaatc
     1561 ccatgataaa gattacagca acgtgaacct gcacgaacat gccgaagcgc acagtgaact
     1621 gccgcgtcag gcgaaataat aa
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